Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1614 and Pf1N1B4_1615 are separated by 55 nucleotides Pf1N1B4_1615 and Pf1N1B4_1616 are separated by 1 nucleotides Pf1N1B4_1616 and Pf1N1B4_1617 are separated by 152 nucleotides Pf1N1B4_1617 and Pf1N1B4_1618 are separated by 230 nucleotides
Pf1N1B4_1614 - Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state), at 1,858,990 to 1,859,475
_1614
Pf1N1B4_1615 - Glutaredoxin 3 (Grx3), at 1,859,531 to 1,859,785
_1615
Pf1N1B4_1616 - FIG136845: Rhodanese-related sulfurtransferase, at 1,859,787 to 1,860,200
_1616
Pf1N1B4_1617 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1), at 1,860,353 to 1,861,882
_1617
Pf1N1B4_1618 - Lipoprotein NlpD, at 1,862,113 to 1,863,405
_1618
Position (kb)
1860
1861
1862 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1859.405 kb on + strand at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.503 kb on - strand at 1859.504 kb on + strand at 1859.504 kb on + strand at 1859.504 kb on + strand at 1859.504 kb on + strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.514 kb on + strand at 1859.515 kb on - strand at 1859.515 kb on - strand at 1859.523 kb on + strand at 1859.523 kb on + strand at 1859.523 kb on + strand at 1859.524 kb on - strand at 1859.635 kb on + strand, within Pf1N1B4_1615 at 1859.635 kb on + strand, within Pf1N1B4_1615 at 1859.636 kb on - strand, within Pf1N1B4_1615 at 1859.636 kb on - strand, within Pf1N1B4_1615 at 1859.636 kb on - strand, within Pf1N1B4_1615 at 1859.728 kb on - strand, within Pf1N1B4_1615 at 1859.728 kb on - strand, within Pf1N1B4_1615 at 1859.728 kb on - strand, within Pf1N1B4_1615 at 1859.767 kb on - strand at 1859.767 kb on - strand at 1859.785 kb on + strand at 1860.222 kb on - strand at 1860.237 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.249 kb on + strand at 1860.250 kb on - strand at 1860.292 kb on + strand at 1860.292 kb on + strand at 1860.292 kb on + strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.299 kb on - strand at 1860.299 kb on - strand at 1860.299 kb on - strand at 1860.307 kb on - strand at 1860.307 kb on - strand at 1860.307 kb on - strand at 1860.330 kb on + strand at 1860.330 kb on + strand at 1860.331 kb on - strand at 1860.331 kb on - strand at 1860.358 kb on + strand at 1860.359 kb on - strand at 1860.434 kb on + strand at 1860.434 kb on + strand at 1860.434 kb on + strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.584 kb on + strand, within Pf1N1B4_1617 at 1860.768 kb on - strand, within Pf1N1B4_1617 at 1860.768 kb on - strand, within Pf1N1B4_1617 at 1860.768 kb on - strand, within Pf1N1B4_1617 at 1860.768 kb on - strand, within Pf1N1B4_1617 at 1860.791 kb on + strand, within Pf1N1B4_1617 at 1860.792 kb on - strand, within Pf1N1B4_1617 at 1860.820 kb on + strand, within Pf1N1B4_1617 at 1860.820 kb on + strand, within Pf1N1B4_1617 at 1860.821 kb on - strand, within Pf1N1B4_1617 at 1860.914 kb on + strand, within Pf1N1B4_1617 at 1860.917 kb on + strand, within Pf1N1B4_1617 at 1860.918 kb on - strand, within Pf1N1B4_1617 at 1860.918 kb on - strand, within Pf1N1B4_1617 at 1860.962 kb on + strand, within Pf1N1B4_1617 at 1860.963 kb on - strand, within Pf1N1B4_1617 at 1860.963 kb on - strand, within Pf1N1B4_1617 at 1861.352 kb on + strand, within Pf1N1B4_1617 at 1861.353 kb on - strand, within Pf1N1B4_1617 at 1861.441 kb on + strand, within Pf1N1B4_1617 at 1861.441 kb on + strand, within Pf1N1B4_1617 at 1861.441 kb on + strand, within Pf1N1B4_1617 at 1861.441 kb on + strand, within Pf1N1B4_1617 at 1861.442 kb on - strand, within Pf1N1B4_1617 at 1861.442 kb on - strand, within Pf1N1B4_1617 at 1861.442 kb on - strand, within Pf1N1B4_1617 at 1861.442 kb on - strand, within Pf1N1B4_1617 at 1861.445 kb on + strand, within Pf1N1B4_1617 at 1861.445 kb on + strand, within Pf1N1B4_1617 at 1861.445 kb on + strand, within Pf1N1B4_1617 at 1861.445 kb on + strand, within Pf1N1B4_1617 at 1861.445 kb on + strand, within Pf1N1B4_1617 at 1861.445 kb on + strand, within Pf1N1B4_1617 at 1861.446 kb on - strand, within Pf1N1B4_1617 at 1861.446 kb on - strand, within Pf1N1B4_1617 at 1861.446 kb on - strand, within Pf1N1B4_1617 at 1861.446 kb on - strand, within Pf1N1B4_1617 at 1861.446 kb on - strand, within Pf1N1B4_1617 at 1861.517 kb on + strand, within Pf1N1B4_1617 at 1861.518 kb on - strand, within Pf1N1B4_1617 at 1861.538 kb on + strand, within Pf1N1B4_1617 at 1861.538 kb on + strand, within Pf1N1B4_1617 at 1861.539 kb on - strand, within Pf1N1B4_1617 at 1861.539 kb on - strand, within Pf1N1B4_1617 at 1861.539 kb on - strand, within Pf1N1B4_1617 at 1861.539 kb on - strand, within Pf1N1B4_1617 at 1861.572 kb on + strand, within Pf1N1B4_1617 at 1861.573 kb on - strand, within Pf1N1B4_1617 at 1861.573 kb on - strand, within Pf1N1B4_1617 at 1861.732 kb on + strand at 1861.732 kb on + strand at 1861.732 kb on + strand at 1861.732 kb on + strand at 1861.733 kb on - strand at 1861.733 kb on - strand at 1861.733 kb on - strand at 1861.733 kb on - strand at 1861.733 kb on - strand at 1861.757 kb on + strand at 1861.757 kb on + strand at 1861.757 kb on + strand at 1861.757 kb on + strand at 1861.757 kb on + strand at 1861.757 kb on + strand at 1861.757 kb on + strand at 1861.757 kb on + strand at 1861.757 kb on + strand at 1861.758 kb on - strand at 1861.758 kb on - strand at 1861.758 kb on - strand at 1861.758 kb on - strand at 1861.758 kb on - strand at 1861.758 kb on - strand at 1862.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 1,859,405 + +0.5 1,859,406 - Pf1N1B4_1614 0.86 -1.3 1,859,406 - Pf1N1B4_1614 0.86 -2.0 1,859,406 - Pf1N1B4_1614 0.86 -0.8 1,859,406 - Pf1N1B4_1614 0.86 +0.6 1,859,406 - Pf1N1B4_1614 0.86 -1.5 1,859,406 - Pf1N1B4_1614 0.86 -1.1 1,859,503 - -2.7 1,859,504 + -3.3 1,859,504 + +0.5 1,859,504 + -2.7 1,859,504 + -0.8 1,859,505 - -0.5 1,859,505 - -1.5 1,859,505 - -1.8 1,859,505 - -2.3 1,859,505 - -0.5 1,859,505 - +0.3 1,859,505 - -2.0 1,859,505 - -1.5 1,859,514 + +0.9 1,859,515 - +0.1 1,859,515 - +1.7 1,859,523 + -1.3 1,859,523 + -1.7 1,859,523 + -1.7 1,859,524 - -2.0 1,859,635 + Pf1N1B4_1615 0.41 -1.5 1,859,635 + Pf1N1B4_1615 0.41 -1.8 1,859,636 - Pf1N1B4_1615 0.41 -0.8 1,859,636 - Pf1N1B4_1615 0.41 -0.0 1,859,636 - Pf1N1B4_1615 0.41 +0.0 1,859,728 - Pf1N1B4_1615 0.77 +2.8 1,859,728 - Pf1N1B4_1615 0.77 -1.8 1,859,728 - Pf1N1B4_1615 0.77 +0.6 1,859,767 - -1.5 1,859,767 - -0.5 1,859,785 + -2.2 1,860,222 - -1.5 1,860,237 - -0.2 1,860,239 - +0.5 1,860,239 - +2.1 1,860,239 - -2.1 1,860,239 - +0.2 1,860,239 - +0.0 1,860,239 - -3.3 1,860,239 - -1.3 1,860,239 - +1.5 1,860,239 - -1.7 1,860,239 - -1.8 1,860,249 + +0.7 1,860,250 - -1.8 1,860,292 + -1.5 1,860,292 + -0.1 1,860,292 + -1.8 1,860,293 - -2.1 1,860,293 - +0.1 1,860,293 - -1.8 1,860,293 - -0.8 1,860,293 - -0.3 1,860,293 - -0.5 1,860,293 - -0.6 1,860,293 - -1.2 1,860,293 - -0.3 1,860,293 - -1.1 1,860,293 - -1.1 1,860,293 - -1.5 1,860,299 - -1.2 1,860,299 - +1.5 1,860,299 - -2.0 1,860,307 - -2.1 1,860,307 - -1.1 1,860,307 - -0.4 1,860,330 + +1.1 1,860,330 + +1.1 1,860,331 - -1.1 1,860,331 - -0.8 1,860,358 + -0.5 1,860,359 - -0.8 1,860,434 + -0.5 1,860,434 + -1.7 1,860,434 + +0.9 1,860,435 - +0.6 1,860,435 - -2.1 1,860,435 - -0.8 1,860,435 - -1.7 1,860,435 - -2.7 1,860,435 - -1.3 1,860,435 - -1.5 1,860,435 - -0.8 1,860,584 + Pf1N1B4_1617 0.15 -0.5 1,860,768 - Pf1N1B4_1617 0.27 -2.1 1,860,768 - Pf1N1B4_1617 0.27 +0.7 1,860,768 - Pf1N1B4_1617 0.27 -1.8 1,860,768 - Pf1N1B4_1617 0.27 -1.3 1,860,791 + Pf1N1B4_1617 0.29 +0.3 1,860,792 - Pf1N1B4_1617 0.29 -1.1 1,860,820 + Pf1N1B4_1617 0.31 -2.0 1,860,820 + Pf1N1B4_1617 0.31 -0.5 1,860,821 - Pf1N1B4_1617 0.31 -0.8 1,860,914 + Pf1N1B4_1617 0.37 -0.5 1,860,917 + Pf1N1B4_1617 0.37 -1.7 1,860,918 - Pf1N1B4_1617 0.37 -2.2 1,860,918 - Pf1N1B4_1617 0.37 -1.1 1,860,962 + Pf1N1B4_1617 0.40 -3.0 1,860,963 - Pf1N1B4_1617 0.40 -1.3 1,860,963 - Pf1N1B4_1617 0.40 -1.3 1,861,352 + Pf1N1B4_1617 0.65 -2.5 1,861,353 - Pf1N1B4_1617 0.65 -1.7 1,861,441 + Pf1N1B4_1617 0.71 -2.1 1,861,441 + Pf1N1B4_1617 0.71 -0.8 1,861,441 + Pf1N1B4_1617 0.71 -0.1 1,861,441 + Pf1N1B4_1617 0.71 -1.1 1,861,442 - Pf1N1B4_1617 0.71 -0.8 1,861,442 - Pf1N1B4_1617 0.71 -0.8 1,861,442 - Pf1N1B4_1617 0.71 +0.3 1,861,442 - Pf1N1B4_1617 0.71 -1.1 1,861,445 + Pf1N1B4_1617 0.71 -1.5 1,861,445 + Pf1N1B4_1617 0.71 -1.5 1,861,445 + Pf1N1B4_1617 0.71 -2.4 1,861,445 + Pf1N1B4_1617 0.71 -1.8 1,861,445 + Pf1N1B4_1617 0.71 -0.8 1,861,445 + Pf1N1B4_1617 0.71 -1.3 1,861,446 - Pf1N1B4_1617 0.71 -2.2 1,861,446 - Pf1N1B4_1617 0.71 -1.7 1,861,446 - Pf1N1B4_1617 0.71 -1.1 1,861,446 - Pf1N1B4_1617 0.71 -2.0 1,861,446 - Pf1N1B4_1617 0.71 -1.5 1,861,517 + Pf1N1B4_1617 0.76 -2.2 1,861,518 - Pf1N1B4_1617 0.76 -1.5 1,861,538 + Pf1N1B4_1617 0.77 -3.0 1,861,538 + Pf1N1B4_1617 0.77 -2.2 1,861,539 - Pf1N1B4_1617 0.78 -2.2 1,861,539 - Pf1N1B4_1617 0.78 -0.5 1,861,539 - Pf1N1B4_1617 0.78 -0.8 1,861,539 - Pf1N1B4_1617 0.78 -1.7 1,861,572 + Pf1N1B4_1617 0.80 -2.5 1,861,573 - Pf1N1B4_1617 0.80 -1.7 1,861,573 - Pf1N1B4_1617 0.80 +1.5 1,861,732 + -0.7 1,861,732 + -1.5 1,861,732 + -3.0 1,861,732 + -1.1 1,861,733 - -1.7 1,861,733 - -0.3 1,861,733 - -0.5 1,861,733 - -2.9 1,861,733 - -1.5 1,861,757 + -1.5 1,861,757 + -1.8 1,861,757 + +1.0 1,861,757 + -1.1 1,861,757 + -2.1 1,861,757 + -1.1 1,861,757 + -1.5 1,861,757 + -1.7 1,861,757 + -2.0 1,861,758 - +0.1 1,861,758 - -0.5 1,861,758 - -2.6 1,861,758 - +0.2 1,861,758 - -0.5 1,861,758 - -0.8 1,862,079 + +0.2
Or see this region's nucleotide sequence