Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1267 and Pf1N1B4_1268 are separated by 128 nucleotides Pf1N1B4_1268 and Pf1N1B4_1269 are separated by 83 nucleotides
Pf1N1B4_1267 - Methyl-accepting chemotaxis protein, at 1,447,467 to 1,449,413
_1267
Pf1N1B4_1268 - Transcriptional regulator, AraC family, at 1,449,542 to 1,450,333
_1268
Pf1N1B4_1269 - Sodium/bile acid symporter family, at 1,450,417 to 1,451,445
_1269
Position (kb)
1449
1450
1451 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1449.469 kb on + strand at 1449.598 kb on + strand at 1449.599 kb on - strand at 1449.619 kb on - strand at 1449.689 kb on + strand, within Pf1N1B4_1268 at 1449.689 kb on + strand, within Pf1N1B4_1268 at 1449.788 kb on + strand, within Pf1N1B4_1268 at 1449.788 kb on + strand, within Pf1N1B4_1268 at 1449.788 kb on + strand, within Pf1N1B4_1268 at 1449.788 kb on + strand, within Pf1N1B4_1268 at 1449.789 kb on - strand, within Pf1N1B4_1268 at 1449.789 kb on - strand, within Pf1N1B4_1268 at 1449.949 kb on - strand, within Pf1N1B4_1268 at 1450.056 kb on + strand, within Pf1N1B4_1268 at 1450.057 kb on - strand at 1450.057 kb on - strand, within Pf1N1B4_1268 at 1450.191 kb on + strand, within Pf1N1B4_1268 at 1450.191 kb on + strand, within Pf1N1B4_1268 at 1450.263 kb on + strand at 1450.263 kb on + strand at 1450.263 kb on + strand at 1450.263 kb on + strand at 1450.263 kb on + strand at 1450.263 kb on + strand at 1450.263 kb on + strand at 1450.263 kb on + strand at 1450.264 kb on - strand at 1450.264 kb on - strand at 1450.264 kb on - strand at 1450.264 kb on - strand at 1450.264 kb on - strand at 1450.264 kb on - strand at 1450.344 kb on + strand at 1450.344 kb on + strand at 1450.344 kb on + strand at 1450.344 kb on + strand at 1450.344 kb on + strand at 1450.345 kb on - strand at 1450.345 kb on - strand at 1450.345 kb on - strand at 1450.381 kb on - strand at 1450.396 kb on - strand at 1450.396 kb on - strand at 1450.404 kb on - strand at 1450.404 kb on - strand at 1450.495 kb on + strand at 1450.495 kb on + strand at 1450.495 kb on + strand at 1450.495 kb on + strand at 1450.496 kb on - strand at 1450.496 kb on - strand at 1450.496 kb on - strand at 1450.496 kb on - strand at 1450.496 kb on - strand at 1450.543 kb on + strand, within Pf1N1B4_1269 at 1450.544 kb on - strand, within Pf1N1B4_1269 at 1450.747 kb on + strand at 1450.747 kb on + strand, within Pf1N1B4_1269 at 1450.747 kb on + strand, within Pf1N1B4_1269 at 1450.748 kb on - strand, within Pf1N1B4_1269 at 1450.748 kb on - strand, within Pf1N1B4_1269 at 1450.748 kb on - strand, within Pf1N1B4_1269 at 1450.814 kb on + strand, within Pf1N1B4_1269 at 1450.814 kb on + strand, within Pf1N1B4_1269 at 1450.814 kb on + strand, within Pf1N1B4_1269 at 1450.815 kb on - strand, within Pf1N1B4_1269 at 1450.815 kb on - strand, within Pf1N1B4_1269 at 1450.850 kb on + strand, within Pf1N1B4_1269 at 1450.850 kb on + strand, within Pf1N1B4_1269 at 1450.850 kb on + strand, within Pf1N1B4_1269 at 1450.940 kb on + strand, within Pf1N1B4_1269 at 1450.940 kb on + strand, within Pf1N1B4_1269 at 1450.955 kb on + strand, within Pf1N1B4_1269 at 1451.065 kb on + strand, within Pf1N1B4_1269
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 1,449,469 + +1.6 1,449,598 + -0.3 1,449,599 - +3.4 1,449,619 - +1.1 1,449,689 + Pf1N1B4_1268 0.19 -1.9 1,449,689 + Pf1N1B4_1268 0.19 -0.5 1,449,788 + Pf1N1B4_1268 0.31 -0.1 1,449,788 + Pf1N1B4_1268 0.31 -2.5 1,449,788 + Pf1N1B4_1268 0.31 -1.8 1,449,788 + Pf1N1B4_1268 0.31 +0.4 1,449,789 - Pf1N1B4_1268 0.31 -1.0 1,449,789 - Pf1N1B4_1268 0.31 -1.8 1,449,949 - Pf1N1B4_1268 0.51 +0.4 1,450,056 + Pf1N1B4_1268 0.65 -0.5 1,450,057 - -0.6 1,450,057 - Pf1N1B4_1268 0.65 -0.4 1,450,191 + Pf1N1B4_1268 0.82 -1.8 1,450,191 + Pf1N1B4_1268 0.82 -1.7 1,450,263 + -0.2 1,450,263 + -1.2 1,450,263 + +0.8 1,450,263 + -0.2 1,450,263 + -0.2 1,450,263 + +1.4 1,450,263 + +0.0 1,450,263 + +2.0 1,450,264 - +2.6 1,450,264 - -1.8 1,450,264 - +0.9 1,450,264 - -0.6 1,450,264 - +1.5 1,450,264 - +1.0 1,450,344 + -0.0 1,450,344 + -0.6 1,450,344 + +2.1 1,450,344 + -0.1 1,450,344 + -2.6 1,450,345 - -0.6 1,450,345 - -0.7 1,450,345 - +0.7 1,450,381 - -1.0 1,450,396 - +0.9 1,450,396 - -1.2 1,450,404 - +0.2 1,450,404 - -0.1 1,450,495 + -0.2 1,450,495 + +0.4 1,450,495 + +0.8 1,450,495 + +0.0 1,450,496 - -3.3 1,450,496 - -1.4 1,450,496 - +0.4 1,450,496 - +1.0 1,450,496 - -0.0 1,450,543 + Pf1N1B4_1269 0.12 -0.2 1,450,544 - Pf1N1B4_1269 0.12 -1.2 1,450,747 + -0.2 1,450,747 + Pf1N1B4_1269 0.32 -2.2 1,450,747 + Pf1N1B4_1269 0.32 -1.8 1,450,748 - Pf1N1B4_1269 0.32 +1.4 1,450,748 - Pf1N1B4_1269 0.32 +0.4 1,450,748 - Pf1N1B4_1269 0.32 +2.0 1,450,814 + Pf1N1B4_1269 0.39 +0.8 1,450,814 + Pf1N1B4_1269 0.39 +0.4 1,450,814 + Pf1N1B4_1269 0.39 -0.6 1,450,815 - Pf1N1B4_1269 0.39 -0.4 1,450,815 - Pf1N1B4_1269 0.39 -1.4 1,450,850 + Pf1N1B4_1269 0.42 -1.0 1,450,850 + Pf1N1B4_1269 0.42 -1.6 1,450,850 + Pf1N1B4_1269 0.42 +2.0 1,450,940 + Pf1N1B4_1269 0.51 +0.7 1,450,940 + Pf1N1B4_1269 0.51 -1.4 1,450,955 + Pf1N1B4_1269 0.52 -0.2 1,451,065 + Pf1N1B4_1269 0.63 -0.6
Or see this region's nucleotide sequence