Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1212 and Pf1N1B4_1213 are separated by 84 nucleotides Pf1N1B4_1213 and Pf1N1B4_1214 are separated by 138 nucleotides Pf1N1B4_1214 and Pf1N1B4_1215 are separated by 80 nucleotides Pf1N1B4_1215 and Pf1N1B4_1216 are separated by 149 nucleotides
Pf1N1B4_1212 - Predicted flavin reductase RutF in novel pyrimidine catabolism pathway, at 1,375,077 to 1,375,379
_1212
Pf1N1B4_1213 - Transcriptional regulator, at 1,375,464 to 1,375,739
_1213
Pf1N1B4_1214 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 1,375,878 to 1,376,408
_1214
Pf1N1B4_1215 - Transcriptional regulator, ArsR family, at 1,376,489 to 1,376,851
_1215
Pf1N1B4_1216 - Outer membrane component of tripartite multidrug resistance system, at 1,377,001 to 1,378,431
_1216
Position (kb)
1375
1376
1377 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1374.897 kb on - strand at 1375.320 kb on + strand, within Pf1N1B4_1212 at 1375.321 kb on - strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.469 kb on - strand at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.495 kb on + strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.514 kb on + strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.547 kb on + strand, within Pf1N1B4_1213 at 1375.717 kb on + strand at 1375.717 kb on + strand at 1375.718 kb on - strand at 1375.718 kb on - strand at 1375.817 kb on - strand at 1375.817 kb on - strand at 1375.817 kb on - strand at 1375.826 kb on + strand at 1375.994 kb on - strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.101 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.364 kb on + strand at 1376.365 kb on - strand at 1376.409 kb on - strand at 1376.409 kb on - strand at 1376.478 kb on - strand at 1376.478 kb on - strand at 1376.478 kb on - strand at 1376.478 kb on - strand at 1376.478 kb on - strand at 1376.641 kb on - strand, within Pf1N1B4_1215 at 1376.811 kb on - strand, within Pf1N1B4_1215 at 1376.814 kb on - strand, within Pf1N1B4_1215 at 1376.851 kb on + strand at 1376.851 kb on + strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.852 kb on - strand at 1376.856 kb on - strand at 1376.856 kb on - strand at 1376.856 kb on - strand at 1376.856 kb on - strand at 1376.860 kb on - strand at 1376.860 kb on - strand at 1376.860 kb on - strand at 1376.872 kb on - strand at 1376.872 kb on - strand at 1376.872 kb on - strand at 1376.874 kb on - strand at 1376.874 kb on - strand at 1376.874 kb on - strand at 1376.890 kb on - strand at 1376.893 kb on - strand at 1376.893 kb on - strand at 1376.893 kb on - strand at 1376.904 kb on - strand at 1376.917 kb on - strand at 1376.917 kb on - strand at 1376.936 kb on - strand at 1376.936 kb on - strand at 1376.965 kb on - strand at 1376.965 kb on - strand at 1376.965 kb on - strand at 1376.965 kb on - strand at 1377.144 kb on + strand at 1377.144 kb on + strand, within Pf1N1B4_1216 at 1377.144 kb on + strand, within Pf1N1B4_1216 at 1377.144 kb on + strand, within Pf1N1B4_1216 at 1377.144 kb on + strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.145 kb on - strand at 1377.145 kb on - strand, within Pf1N1B4_1216 at 1377.162 kb on - strand, within Pf1N1B4_1216 at 1377.162 kb on - strand, within Pf1N1B4_1216 at 1377.182 kb on + strand, within Pf1N1B4_1216 at 1377.182 kb on + strand, within Pf1N1B4_1216 at 1377.183 kb on - strand, within Pf1N1B4_1216 at 1377.183 kb on - strand, within Pf1N1B4_1216 at 1377.183 kb on - strand, within Pf1N1B4_1216 at 1377.289 kb on + strand, within Pf1N1B4_1216 at 1377.290 kb on - strand, within Pf1N1B4_1216 at 1377.290 kb on - strand, within Pf1N1B4_1216 at 1377.299 kb on + strand, within Pf1N1B4_1216 at 1377.300 kb on - strand, within Pf1N1B4_1216 at 1377.315 kb on + strand, within Pf1N1B4_1216 at 1377.316 kb on - strand, within Pf1N1B4_1216
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 1,374,897 - +0.1 1,375,320 + Pf1N1B4_1212 0.80 -1.6 1,375,321 - Pf1N1B4_1212 0.81 -2.3 1,375,335 + Pf1N1B4_1212 0.85 -0.1 1,375,335 + Pf1N1B4_1212 0.85 -1.7 1,375,335 + Pf1N1B4_1212 0.85 -0.3 1,375,335 + Pf1N1B4_1212 0.85 -0.0 1,375,335 + Pf1N1B4_1212 0.85 -1.5 1,375,335 + Pf1N1B4_1212 0.85 -0.7 1,375,336 - Pf1N1B4_1212 0.85 +1.9 1,375,336 - Pf1N1B4_1212 0.85 -0.3 1,375,469 - -0.8 1,375,494 - Pf1N1B4_1213 0.11 -0.3 1,375,494 - Pf1N1B4_1213 0.11 -1.0 1,375,495 + Pf1N1B4_1213 0.11 +1.1 1,375,496 - Pf1N1B4_1213 0.12 -0.3 1,375,496 - Pf1N1B4_1213 0.12 -3.0 1,375,502 + Pf1N1B4_1213 0.14 +0.3 1,375,502 + Pf1N1B4_1213 0.14 +1.3 1,375,503 - Pf1N1B4_1213 0.14 -1.7 1,375,503 - Pf1N1B4_1213 0.14 +0.1 1,375,503 - Pf1N1B4_1213 0.14 +0.9 1,375,503 - Pf1N1B4_1213 0.14 -0.1 1,375,503 - Pf1N1B4_1213 0.14 -0.6 1,375,514 + Pf1N1B4_1213 0.18 -1.5 1,375,515 - Pf1N1B4_1213 0.18 +0.6 1,375,515 - Pf1N1B4_1213 0.18 +0.3 1,375,515 - Pf1N1B4_1213 0.18 -0.7 1,375,515 - Pf1N1B4_1213 0.18 +0.3 1,375,547 + Pf1N1B4_1213 0.30 -1.0 1,375,717 + -1.3 1,375,717 + -1.3 1,375,718 - -1.3 1,375,718 - +0.7 1,375,817 - -1.0 1,375,817 - +0.8 1,375,817 - -2.4 1,375,826 + -1.3 1,375,994 - Pf1N1B4_1214 0.22 -1.3 1,376,069 + Pf1N1B4_1214 0.36 -1.5 1,376,069 + Pf1N1B4_1214 0.36 +0.7 1,376,069 + Pf1N1B4_1214 0.36 -0.3 1,376,069 + Pf1N1B4_1214 0.36 +1.5 1,376,069 + Pf1N1B4_1214 0.36 -2.0 1,376,069 + Pf1N1B4_1214 0.36 +0.4 1,376,069 + Pf1N1B4_1214 0.36 -0.7 1,376,069 + Pf1N1B4_1214 0.36 +0.7 1,376,070 - Pf1N1B4_1214 0.36 -1.3 1,376,070 - Pf1N1B4_1214 0.36 +0.3 1,376,070 - Pf1N1B4_1214 0.36 +0.1 1,376,070 - Pf1N1B4_1214 0.36 -1.2 1,376,070 - Pf1N1B4_1214 0.36 +1.6 1,376,070 - Pf1N1B4_1214 0.36 -0.1 1,376,070 - Pf1N1B4_1214 0.36 -0.5 1,376,070 - Pf1N1B4_1214 0.36 -0.4 1,376,101 + Pf1N1B4_1214 0.42 +3.1 1,376,293 + Pf1N1B4_1214 0.78 -0.7 1,376,293 + Pf1N1B4_1214 0.78 +2.0 1,376,293 + Pf1N1B4_1214 0.78 +0.6 1,376,364 + +0.0 1,376,365 - -0.7 1,376,409 - -0.7 1,376,409 - -0.3 1,376,478 - -0.5 1,376,478 - +0.1 1,376,478 - -1.7 1,376,478 - -2.4 1,376,478 - +1.3 1,376,641 - Pf1N1B4_1215 0.42 -1.9 1,376,811 - Pf1N1B4_1215 0.89 -2.0 1,376,814 - Pf1N1B4_1215 0.90 -0.1 1,376,851 + -0.8 1,376,851 + -0.3 1,376,852 - -2.1 1,376,852 - -0.7 1,376,852 - +0.3 1,376,852 - -0.7 1,376,852 - -0.7 1,376,852 - +0.3 1,376,852 - -1.3 1,376,852 - +2.3 1,376,856 - +0.6 1,376,856 - -1.3 1,376,856 - -2.0 1,376,856 - -0.3 1,376,860 - -0.4 1,376,860 - -1.1 1,376,860 - -0.0 1,376,872 - -1.3 1,376,872 - +1.7 1,376,872 - -0.7 1,376,874 - +1.3 1,376,874 - -0.3 1,376,874 - -0.1 1,376,890 - -0.6 1,376,893 - +0.5 1,376,893 - -0.3 1,376,893 - -0.3 1,376,904 - -1.7 1,376,917 - +1.1 1,376,917 - -0.2 1,376,936 - -1.0 1,376,936 - -1.3 1,376,965 - -1.0 1,376,965 - +0.3 1,376,965 - -2.0 1,376,965 - -0.6 1,377,144 + -0.3 1,377,144 + Pf1N1B4_1216 0.10 -1.3 1,377,144 + Pf1N1B4_1216 0.10 +0.1 1,377,144 + Pf1N1B4_1216 0.10 -1.3 1,377,144 + Pf1N1B4_1216 0.10 -0.3 1,377,145 - Pf1N1B4_1216 0.10 -0.6 1,377,145 - Pf1N1B4_1216 0.10 -1.3 1,377,145 - Pf1N1B4_1216 0.10 -0.7 1,377,145 - Pf1N1B4_1216 0.10 -1.0 1,377,145 - Pf1N1B4_1216 0.10 -1.7 1,377,145 - +1.3 1,377,145 - Pf1N1B4_1216 0.10 -1.5 1,377,162 - Pf1N1B4_1216 0.11 +1.3 1,377,162 - Pf1N1B4_1216 0.11 -0.3 1,377,182 + Pf1N1B4_1216 0.13 +1.3 1,377,182 + Pf1N1B4_1216 0.13 +1.7 1,377,183 - Pf1N1B4_1216 0.13 -1.3 1,377,183 - Pf1N1B4_1216 0.13 -2.4 1,377,183 - Pf1N1B4_1216 0.13 -1.4 1,377,289 + Pf1N1B4_1216 0.20 -2.6 1,377,290 - Pf1N1B4_1216 0.20 -1.4 1,377,290 - Pf1N1B4_1216 0.20 -0.1 1,377,299 + Pf1N1B4_1216 0.21 +1.6 1,377,300 - Pf1N1B4_1216 0.21 +0.6 1,377,315 + Pf1N1B4_1216 0.22 +0.6 1,377,316 - Pf1N1B4_1216 0.22 -1.3
Or see this region's nucleotide sequence