Experiment: root sample 4; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS02295 and ABZR87_RS02300 are separated by 158 nucleotides ABZR87_RS02300 and ABZR87_RS02305 are separated by 9 nucleotides ABZR87_RS02305 and ABZR87_RS02310 overlap by 4 nucleotides ABZR87_RS02310 and ABZR87_RS02315 are separated by 124 nucleotides
ABZR87_RS02295: ABZR87_RS02295 - hypothetical protein, at 492,628 to 492,939
_RS02295
ABZR87_RS02300: ABZR87_RS02300 - aminoacyl-tRNA deacylase, at 493,098 to 493,586
_RS02300
ABZR87_RS02305: ABZR87_RS02305 - CDGSH iron-sulfur domain-containing protein, at 493,596 to 493,820
_RS02305
ABZR87_RS02310: ABZR87_RS02310 - energy-dependent translational throttle protein EttA, at 493,817 to 495,484
_RS02310
ABZR87_RS02315: ABZR87_RS02315 - winged helix DNA-binding domain-containing protein, at 495,609 to 496,739
_RS02315
Position (kb) 493
494
495
496 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 492.823 kb on + strand, within ABZR87_RS02295 at 492.832 kb on + strand, within ABZR87_RS02295 at 492.832 kb on + strand, within ABZR87_RS02295 at 492.832 kb on + strand, within ABZR87_RS02295 at 492.835 kb on + strand, within ABZR87_RS02295 at 492.851 kb on - strand, within ABZR87_RS02295 at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.038 kb on + strand at 493.039 kb on - strand at 493.039 kb on - strand at 493.039 kb on - strand at 493.039 kb on - strand at 493.039 kb on - strand at 493.039 kb on - strand at 493.048 kb on + strand at 493.049 kb on - strand at 493.049 kb on - strand at 493.149 kb on + strand, within ABZR87_RS02300 at 493.150 kb on - strand, within ABZR87_RS02300 at 493.150 kb on - strand, within ABZR87_RS02300 at 493.150 kb on - strand, within ABZR87_RS02300 at 493.150 kb on - strand, within ABZR87_RS02300 at 493.172 kb on + strand, within ABZR87_RS02300 at 493.172 kb on + strand, within ABZR87_RS02300 at 493.178 kb on + strand, within ABZR87_RS02300 at 493.178 kb on + strand, within ABZR87_RS02300 at 493.179 kb on - strand, within ABZR87_RS02300 at 493.179 kb on - strand, within ABZR87_RS02300 at 493.179 kb on - strand, within ABZR87_RS02300 at 493.237 kb on + strand, within ABZR87_RS02300 at 493.237 kb on + strand, within ABZR87_RS02300 at 493.261 kb on - strand, within ABZR87_RS02300 at 493.287 kb on + strand, within ABZR87_RS02300 at 493.287 kb on + strand, within ABZR87_RS02300 at 493.287 kb on + strand, within ABZR87_RS02300 at 493.287 kb on + strand, within ABZR87_RS02300 at 493.287 kb on + strand, within ABZR87_RS02300 at 493.287 kb on + strand, within ABZR87_RS02300 at 493.287 kb on + strand, within ABZR87_RS02300 at 493.288 kb on - strand, within ABZR87_RS02300 at 493.288 kb on - strand, within ABZR87_RS02300 at 493.288 kb on - strand, within ABZR87_RS02300 at 493.288 kb on - strand, within ABZR87_RS02300 at 493.288 kb on - strand, within ABZR87_RS02300 at 493.417 kb on - strand, within ABZR87_RS02300 at 493.417 kb on - strand, within ABZR87_RS02300 at 493.417 kb on - strand, within ABZR87_RS02300 at 493.431 kb on + strand, within ABZR87_RS02300 at 493.431 kb on + strand, within ABZR87_RS02300 at 493.431 kb on + strand, within ABZR87_RS02300 at 493.431 kb on + strand, within ABZR87_RS02300 at 493.432 kb on - strand, within ABZR87_RS02300 at 493.432 kb on - strand, within ABZR87_RS02300 at 493.432 kb on - strand, within ABZR87_RS02300 at 493.432 kb on - strand, within ABZR87_RS02300 at 493.432 kb on - strand, within ABZR87_RS02300 at 493.467 kb on + strand, within ABZR87_RS02300 at 493.468 kb on - strand, within ABZR87_RS02300 at 493.468 kb on - strand, within ABZR87_RS02300 at 493.468 kb on - strand, within ABZR87_RS02300 at 493.468 kb on - strand, within ABZR87_RS02300 at 493.502 kb on + strand, within ABZR87_RS02300 at 493.502 kb on + strand, within ABZR87_RS02300 at 493.502 kb on + strand, within ABZR87_RS02300 at 493.503 kb on - strand, within ABZR87_RS02300 at 493.503 kb on - strand, within ABZR87_RS02300 at 493.503 kb on - strand, within ABZR87_RS02300 at 493.503 kb on - strand, within ABZR87_RS02300 at 493.503 kb on - strand, within ABZR87_RS02300 at 493.503 kb on - strand, within ABZR87_RS02300 at 493.503 kb on - strand, within ABZR87_RS02300 at 493.503 kb on - strand, within ABZR87_RS02300 at 493.693 kb on + strand, within ABZR87_RS02305 at 493.694 kb on - strand, within ABZR87_RS02305 at 493.819 kb on - strand at 493.826 kb on - strand at 493.896 kb on + strand at 493.896 kb on + strand at 493.896 kb on + strand at 493.896 kb on + strand at 493.896 kb on + strand at 493.905 kb on + strand at 493.906 kb on - strand at 493.906 kb on - strand at 494.205 kb on + strand, within ABZR87_RS02310 at 494.205 kb on + strand, within ABZR87_RS02310 at 494.205 kb on + strand, within ABZR87_RS02310 at 494.205 kb on + strand, within ABZR87_RS02310 at 494.205 kb on + strand, within ABZR87_RS02310 at 494.206 kb on - strand, within ABZR87_RS02310 at 494.206 kb on - strand, within ABZR87_RS02310 at 494.316 kb on + strand, within ABZR87_RS02310 at 494.317 kb on - strand, within ABZR87_RS02310 at 494.317 kb on - strand, within ABZR87_RS02310 at 494.401 kb on + strand, within ABZR87_RS02310 at 494.401 kb on + strand, within ABZR87_RS02310 at 494.487 kb on + strand, within ABZR87_RS02310 at 494.487 kb on + strand, within ABZR87_RS02310 at 494.487 kb on + strand, within ABZR87_RS02310 at 494.487 kb on + strand, within ABZR87_RS02310 at 494.487 kb on + strand, within ABZR87_RS02310 at 494.488 kb on - strand, within ABZR87_RS02310 at 494.488 kb on - strand, within ABZR87_RS02310 at 494.488 kb on - strand, within ABZR87_RS02310 at 494.488 kb on - strand, within ABZR87_RS02310 at 494.488 kb on - strand, within ABZR87_RS02310 at 494.754 kb on + strand, within ABZR87_RS02310 at 494.754 kb on + strand, within ABZR87_RS02310 at 494.754 kb on + strand, within ABZR87_RS02310 at 494.754 kb on + strand, within ABZR87_RS02310 at 494.754 kb on + strand, within ABZR87_RS02310 at 494.754 kb on + strand, within ABZR87_RS02310 at 494.754 kb on + strand, within ABZR87_RS02310 at 494.755 kb on - strand, within ABZR87_RS02310 at 494.755 kb on - strand, within ABZR87_RS02310 at 494.755 kb on - strand, within ABZR87_RS02310 at 494.755 kb on - strand, within ABZR87_RS02310 at 494.755 kb on - strand, within ABZR87_RS02310 at 494.755 kb on - strand, within ABZR87_RS02310 at 494.755 kb on - strand, within ABZR87_RS02310 at 494.755 kb on - strand, within ABZR87_RS02310 at 494.826 kb on + strand, within ABZR87_RS02310 at 494.826 kb on + strand, within ABZR87_RS02310 at 494.827 kb on - strand, within ABZR87_RS02310 at 495.153 kb on + strand, within ABZR87_RS02310 at 495.154 kb on - strand, within ABZR87_RS02310 at 495.162 kb on + strand, within ABZR87_RS02310 at 495.162 kb on + strand, within ABZR87_RS02310 at 495.162 kb on + strand, within ABZR87_RS02310 at 495.163 kb on - strand, within ABZR87_RS02310 at 495.268 kb on - strand, within ABZR87_RS02310 at 495.268 kb on - strand, within ABZR87_RS02310 at 495.474 kb on + strand at 495.474 kb on + strand at 495.489 kb on + strand at 495.489 kb on + strand at 495.489 kb on + strand at 495.490 kb on - strand at 495.490 kb on - strand at 495.532 kb on + strand at 495.532 kb on + strand at 495.532 kb on + strand at 495.532 kb on + strand at 495.532 kb on + strand at 495.533 kb on - strand at 495.617 kb on + strand at 495.618 kb on - strand at 495.682 kb on + strand at 495.783 kb on + strand, within ABZR87_RS02315 at 495.784 kb on - strand, within ABZR87_RS02315 at 496.054 kb on + strand, within ABZR87_RS02315 at 496.054 kb on + strand, within ABZR87_RS02315 at 496.055 kb on - strand, within ABZR87_RS02315 at 496.254 kb on + strand, within ABZR87_RS02315 at 496.255 kb on - strand, within ABZR87_RS02315 at 496.290 kb on - strand, within ABZR87_RS02315 at 496.339 kb on + strand, within ABZR87_RS02315 at 496.340 kb on - strand, within ABZR87_RS02315 at 496.340 kb on - strand, within ABZR87_RS02315 at 496.340 kb on - strand, within ABZR87_RS02315 at 496.340 kb on - strand, within ABZR87_RS02315 at 496.340 kb on - strand, within ABZR87_RS02315
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 4; outgrowth in LB remove 492,823 + ABZR87_RS02295 0.62 -1.2 492,832 + ABZR87_RS02295 0.65 +0.5 492,832 + ABZR87_RS02295 0.65 -0.2 492,832 + ABZR87_RS02295 0.65 -0.4 492,835 + ABZR87_RS02295 0.66 +0.5 492,851 - ABZR87_RS02295 0.71 +1.7 493,038 + -0.3 493,038 + -0.1 493,038 + +3.0 493,038 + +1.3 493,038 + +1.0 493,038 + +1.7 493,038 + +0.0 493,038 + -0.4 493,038 + +0.6 493,038 + +2.1 493,038 + +0.2 493,038 + -0.1 493,038 + +1.1 493,039 - +1.2 493,039 - -0.5 493,039 - +0.5 493,039 - +0.5 493,039 - -0.5 493,039 - +1.3 493,048 + -0.5 493,049 - -0.0 493,049 - -0.2 493,149 + ABZR87_RS02300 0.10 -0.4 493,150 - ABZR87_RS02300 0.11 -0.6 493,150 - ABZR87_RS02300 0.11 -0.4 493,150 - ABZR87_RS02300 0.11 +0.5 493,150 - ABZR87_RS02300 0.11 -0.1 493,172 + ABZR87_RS02300 0.15 +0.4 493,172 + ABZR87_RS02300 0.15 -0.1 493,178 + ABZR87_RS02300 0.16 -0.1 493,178 + ABZR87_RS02300 0.16 +0.4 493,179 - ABZR87_RS02300 0.17 -0.0 493,179 - ABZR87_RS02300 0.17 +1.8 493,179 - ABZR87_RS02300 0.17 -0.8 493,237 + ABZR87_RS02300 0.28 -0.0 493,237 + ABZR87_RS02300 0.28 -1.8 493,261 - ABZR87_RS02300 0.33 +0.0 493,287 + ABZR87_RS02300 0.39 +0.3 493,287 + ABZR87_RS02300 0.39 -1.2 493,287 + ABZR87_RS02300 0.39 -0.9 493,287 + ABZR87_RS02300 0.39 +0.1 493,287 + ABZR87_RS02300 0.39 +0.4 493,287 + ABZR87_RS02300 0.39 +0.5 493,287 + ABZR87_RS02300 0.39 +1.1 493,288 - ABZR87_RS02300 0.39 +1.0 493,288 - ABZR87_RS02300 0.39 +0.0 493,288 - ABZR87_RS02300 0.39 -1.2 493,288 - ABZR87_RS02300 0.39 -1.3 493,288 - ABZR87_RS02300 0.39 +0.0 493,417 - ABZR87_RS02300 0.65 +1.3 493,417 - ABZR87_RS02300 0.65 -0.0 493,417 - ABZR87_RS02300 0.65 -0.5 493,431 + ABZR87_RS02300 0.68 -0.3 493,431 + ABZR87_RS02300 0.68 +0.4 493,431 + ABZR87_RS02300 0.68 -0.9 493,431 + ABZR87_RS02300 0.68 +0.3 493,432 - ABZR87_RS02300 0.68 +2.1 493,432 - ABZR87_RS02300 0.68 -1.2 493,432 - ABZR87_RS02300 0.68 +0.6 493,432 - ABZR87_RS02300 0.68 +0.2 493,432 - ABZR87_RS02300 0.68 -0.7 493,467 + ABZR87_RS02300 0.75 -0.4 493,468 - ABZR87_RS02300 0.76 +1.0 493,468 - ABZR87_RS02300 0.76 +0.3 493,468 - ABZR87_RS02300 0.76 -0.0 493,468 - ABZR87_RS02300 0.76 +0.5 493,502 + ABZR87_RS02300 0.83 -0.2 493,502 + ABZR87_RS02300 0.83 +1.0 493,502 + ABZR87_RS02300 0.83 -0.2 493,503 - ABZR87_RS02300 0.83 -0.1 493,503 - ABZR87_RS02300 0.83 -0.7 493,503 - ABZR87_RS02300 0.83 +1.2 493,503 - ABZR87_RS02300 0.83 +0.1 493,503 - ABZR87_RS02300 0.83 +0.1 493,503 - ABZR87_RS02300 0.83 +0.4 493,503 - ABZR87_RS02300 0.83 -1.6 493,503 - ABZR87_RS02300 0.83 -0.6 493,693 + ABZR87_RS02305 0.43 +1.0 493,694 - ABZR87_RS02305 0.44 -2.0 493,819 - -0.0 493,826 - -0.5 493,896 + -0.2 493,896 + +0.9 493,896 + -0.0 493,896 + +1.7 493,896 + +0.6 493,905 + -0.1 493,906 - -0.6 493,906 - -2.0 494,205 + ABZR87_RS02310 0.23 -0.3 494,205 + ABZR87_RS02310 0.23 +0.9 494,205 + ABZR87_RS02310 0.23 +0.3 494,205 + ABZR87_RS02310 0.23 +0.3 494,205 + ABZR87_RS02310 0.23 -0.5 494,206 - ABZR87_RS02310 0.23 -1.0 494,206 - ABZR87_RS02310 0.23 -0.1 494,316 + ABZR87_RS02310 0.30 -1.5 494,317 - ABZR87_RS02310 0.30 +0.2 494,317 - ABZR87_RS02310 0.30 -0.6 494,401 + ABZR87_RS02310 0.35 +1.2 494,401 + ABZR87_RS02310 0.35 +0.4 494,487 + ABZR87_RS02310 0.40 +0.8 494,487 + ABZR87_RS02310 0.40 -0.1 494,487 + ABZR87_RS02310 0.40 +0.2 494,487 + ABZR87_RS02310 0.40 +0.5 494,487 + ABZR87_RS02310 0.40 +0.3 494,488 - ABZR87_RS02310 0.40 -0.1 494,488 - ABZR87_RS02310 0.40 -0.6 494,488 - ABZR87_RS02310 0.40 -1.5 494,488 - ABZR87_RS02310 0.40 +0.0 494,488 - ABZR87_RS02310 0.40 -0.1 494,754 + ABZR87_RS02310 0.56 -0.1 494,754 + ABZR87_RS02310 0.56 -0.0 494,754 + ABZR87_RS02310 0.56 +0.3 494,754 + ABZR87_RS02310 0.56 -0.0 494,754 + ABZR87_RS02310 0.56 +0.1 494,754 + ABZR87_RS02310 0.56 -1.2 494,754 + ABZR87_RS02310 0.56 +0.1 494,755 - ABZR87_RS02310 0.56 +0.6 494,755 - ABZR87_RS02310 0.56 +0.5 494,755 - ABZR87_RS02310 0.56 +0.6 494,755 - ABZR87_RS02310 0.56 +0.9 494,755 - ABZR87_RS02310 0.56 +0.0 494,755 - ABZR87_RS02310 0.56 -0.9 494,755 - ABZR87_RS02310 0.56 -0.1 494,755 - ABZR87_RS02310 0.56 +1.8 494,826 + ABZR87_RS02310 0.60 +0.9 494,826 + ABZR87_RS02310 0.60 -0.3 494,827 - ABZR87_RS02310 0.61 +0.5 495,153 + ABZR87_RS02310 0.80 +0.1 495,154 - ABZR87_RS02310 0.80 +0.4 495,162 + ABZR87_RS02310 0.81 +0.3 495,162 + ABZR87_RS02310 0.81 +0.5 495,162 + ABZR87_RS02310 0.81 +1.9 495,163 - ABZR87_RS02310 0.81 -0.2 495,268 - ABZR87_RS02310 0.87 -0.6 495,268 - ABZR87_RS02310 0.87 -1.8 495,474 + +0.3 495,474 + -1.6 495,489 + -0.5 495,489 + -0.9 495,489 + -0.1 495,490 - -1.9 495,490 - +1.7 495,532 + -0.1 495,532 + -0.2 495,532 + +0.6 495,532 + -0.5 495,532 + -1.0 495,533 - +1.0 495,617 + +1.1 495,618 - +0.7 495,682 + -0.3 495,783 + ABZR87_RS02315 0.15 -0.5 495,784 - ABZR87_RS02315 0.15 -0.7 496,054 + ABZR87_RS02315 0.39 +0.6 496,054 + ABZR87_RS02315 0.39 +2.1 496,055 - ABZR87_RS02315 0.39 +0.5 496,254 + ABZR87_RS02315 0.57 +0.3 496,255 - ABZR87_RS02315 0.57 +0.4 496,290 - ABZR87_RS02315 0.60 +0.2 496,339 + ABZR87_RS02315 0.65 +1.1 496,340 - ABZR87_RS02315 0.65 +0.5 496,340 - ABZR87_RS02315 0.65 +0.6 496,340 - ABZR87_RS02315 0.65 -1.4 496,340 - ABZR87_RS02315 0.65 +0.6 496,340 - ABZR87_RS02315 0.65 +1.5
Or see this region's nucleotide sequence