Experiment: root sample 4; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS01995 and ABZR87_RS02000 are separated by 121 nucleotides ABZR87_RS02000 and ABZR87_RS02005 are separated by 87 nucleotides
ABZR87_RS01995: ABZR87_RS01995 - porin, at 425,159 to 426,337
_RS01995
ABZR87_RS02000: ABZR87_RS02000 - tripartite tricarboxylate transporter substrate binding protein, at 426,459 to 427,484
_RS02000
ABZR87_RS02005: ABZR87_RS02005 - MFS transporter, at 427,572 to 428,888
_RS02005
Position (kb) 426
427
428 Strain fitness (log2 ratio) -2
-1
0
1
2 at 425.537 kb on + strand, within ABZR87_RS01995 at 425.537 kb on + strand, within ABZR87_RS01995 at 425.537 kb on + strand, within ABZR87_RS01995 at 425.537 kb on + strand, within ABZR87_RS01995 at 425.537 kb on + strand, within ABZR87_RS01995 at 425.538 kb on - strand, within ABZR87_RS01995 at 425.538 kb on - strand, within ABZR87_RS01995 at 425.538 kb on - strand, within ABZR87_RS01995 at 425.538 kb on - strand, within ABZR87_RS01995 at 425.538 kb on - strand, within ABZR87_RS01995 at 425.538 kb on - strand, within ABZR87_RS01995 at 425.582 kb on + strand, within ABZR87_RS01995 at 425.594 kb on + strand, within ABZR87_RS01995 at 425.594 kb on + strand, within ABZR87_RS01995 at 425.595 kb on - strand, within ABZR87_RS01995 at 425.595 kb on - strand, within ABZR87_RS01995 at 425.595 kb on - strand, within ABZR87_RS01995 at 425.595 kb on - strand, within ABZR87_RS01995 at 425.651 kb on + strand, within ABZR87_RS01995 at 425.651 kb on + strand, within ABZR87_RS01995 at 425.651 kb on + strand, within ABZR87_RS01995 at 425.651 kb on + strand, within ABZR87_RS01995 at 425.651 kb on + strand, within ABZR87_RS01995 at 425.652 kb on - strand, within ABZR87_RS01995 at 425.652 kb on - strand, within ABZR87_RS01995 at 425.753 kb on + strand, within ABZR87_RS01995 at 425.753 kb on + strand, within ABZR87_RS01995 at 425.754 kb on - strand, within ABZR87_RS01995 at 425.754 kb on - strand, within ABZR87_RS01995 at 425.780 kb on - strand, within ABZR87_RS01995 at 425.787 kb on - strand, within ABZR87_RS01995 at 425.843 kb on + strand, within ABZR87_RS01995 at 425.843 kb on + strand, within ABZR87_RS01995 at 425.844 kb on - strand, within ABZR87_RS01995 at 425.879 kb on + strand, within ABZR87_RS01995 at 425.879 kb on + strand, within ABZR87_RS01995 at 425.879 kb on + strand, within ABZR87_RS01995 at 425.880 kb on - strand, within ABZR87_RS01995 at 425.880 kb on - strand, within ABZR87_RS01995 at 425.880 kb on - strand, within ABZR87_RS01995 at 425.919 kb on - strand, within ABZR87_RS01995 at 425.981 kb on + strand, within ABZR87_RS01995 at 425.981 kb on + strand, within ABZR87_RS01995 at 425.982 kb on - strand, within ABZR87_RS01995 at 425.982 kb on - strand, within ABZR87_RS01995 at 425.982 kb on - strand, within ABZR87_RS01995 at 425.982 kb on - strand, within ABZR87_RS01995 at 425.982 kb on - strand, within ABZR87_RS01995 at 425.982 kb on - strand, within ABZR87_RS01995 at 425.984 kb on + strand, within ABZR87_RS01995 at 425.984 kb on + strand, within ABZR87_RS01995 at 425.984 kb on + strand, within ABZR87_RS01995 at 425.984 kb on + strand, within ABZR87_RS01995 at 425.985 kb on - strand, within ABZR87_RS01995 at 425.985 kb on - strand, within ABZR87_RS01995 at 425.985 kb on - strand, within ABZR87_RS01995 at 425.985 kb on - strand, within ABZR87_RS01995 at 426.002 kb on + strand, within ABZR87_RS01995 at 426.002 kb on + strand, within ABZR87_RS01995 at 426.002 kb on + strand, within ABZR87_RS01995 at 426.002 kb on + strand, within ABZR87_RS01995 at 426.003 kb on - strand, within ABZR87_RS01995 at 426.003 kb on - strand, within ABZR87_RS01995 at 426.003 kb on - strand, within ABZR87_RS01995 at 426.038 kb on + strand, within ABZR87_RS01995 at 426.038 kb on + strand, within ABZR87_RS01995 at 426.039 kb on - strand, within ABZR87_RS01995 at 426.041 kb on + strand, within ABZR87_RS01995 at 426.041 kb on + strand, within ABZR87_RS01995 at 426.041 kb on + strand, within ABZR87_RS01995 at 426.041 kb on + strand, within ABZR87_RS01995 at 426.041 kb on + strand, within ABZR87_RS01995 at 426.041 kb on + strand, within ABZR87_RS01995 at 426.042 kb on - strand, within ABZR87_RS01995 at 426.042 kb on - strand, within ABZR87_RS01995 at 426.042 kb on - strand, within ABZR87_RS01995 at 426.042 kb on - strand, within ABZR87_RS01995 at 426.042 kb on - strand, within ABZR87_RS01995 at 426.044 kb on + strand, within ABZR87_RS01995 at 426.045 kb on - strand, within ABZR87_RS01995 at 426.045 kb on - strand, within ABZR87_RS01995 at 426.125 kb on + strand, within ABZR87_RS01995 at 426.125 kb on + strand, within ABZR87_RS01995 at 426.126 kb on - strand, within ABZR87_RS01995 at 426.126 kb on - strand, within ABZR87_RS01995 at 426.152 kb on + strand, within ABZR87_RS01995 at 426.152 kb on + strand, within ABZR87_RS01995 at 426.152 kb on + strand, within ABZR87_RS01995 at 426.153 kb on - strand, within ABZR87_RS01995 at 426.153 kb on - strand, within ABZR87_RS01995 at 426.153 kb on - strand, within ABZR87_RS01995 at 426.153 kb on - strand, within ABZR87_RS01995 at 426.167 kb on + strand, within ABZR87_RS01995 at 426.206 kb on + strand, within ABZR87_RS01995 at 426.207 kb on - strand, within ABZR87_RS01995 at 426.207 kb on - strand, within ABZR87_RS01995 at 426.248 kb on + strand at 426.248 kb on + strand at 426.272 kb on + strand at 426.272 kb on + strand at 426.272 kb on + strand at 426.272 kb on + strand at 426.272 kb on + strand at 426.272 kb on + strand at 426.273 kb on - strand at 426.273 kb on - strand at 426.273 kb on - strand at 426.273 kb on - strand at 426.273 kb on - strand at 426.273 kb on - strand at 426.273 kb on - strand at 426.422 kb on + strand at 426.422 kb on + strand at 426.745 kb on - strand, within ABZR87_RS02000 at 426.943 kb on - strand, within ABZR87_RS02000 at 427.059 kb on + strand, within ABZR87_RS02000 at 427.059 kb on + strand, within ABZR87_RS02000 at 427.059 kb on + strand, within ABZR87_RS02000 at 427.059 kb on + strand, within ABZR87_RS02000 at 427.060 kb on - strand, within ABZR87_RS02000 at 427.248 kb on + strand, within ABZR87_RS02000 at 427.249 kb on - strand, within ABZR87_RS02000 at 427.249 kb on - strand, within ABZR87_RS02000 at 427.249 kb on - strand, within ABZR87_RS02000 at 427.249 kb on - strand, within ABZR87_RS02000 at 427.249 kb on - strand, within ABZR87_RS02000 at 427.537 kb on + strand at 427.538 kb on - strand at 427.538 kb on - strand at 427.622 kb on + strand at 427.622 kb on + strand at 427.622 kb on + strand at 427.622 kb on + strand at 427.683 kb on + strand at 427.683 kb on + strand at 427.684 kb on - strand at 427.684 kb on - strand at 427.691 kb on - strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.692 kb on + strand at 427.693 kb on - strand at 427.693 kb on - strand at 427.693 kb on - strand at 427.693 kb on - strand at 427.693 kb on - strand at 427.693 kb on - strand at 427.698 kb on + strand at 427.698 kb on + strand at 427.698 kb on + strand at 427.698 kb on + strand at 427.698 kb on + strand at 427.699 kb on - strand at 427.699 kb on - strand at 427.699 kb on - strand at 427.699 kb on - strand at 427.699 kb on - strand at 427.699 kb on - strand at 427.699 kb on - strand at 427.800 kb on - strand, within ABZR87_RS02005 at 427.879 kb on + strand, within ABZR87_RS02005 at 427.880 kb on - strand, within ABZR87_RS02005 at 427.880 kb on - strand, within ABZR87_RS02005 at 427.880 kb on - strand, within ABZR87_RS02005 at 427.880 kb on - strand, within ABZR87_RS02005 at 427.880 kb on - strand, within ABZR87_RS02005 at 427.926 kb on + strand, within ABZR87_RS02005 at 427.926 kb on + strand, within ABZR87_RS02005 at 427.927 kb on - strand, within ABZR87_RS02005 at 428.140 kb on + strand, within ABZR87_RS02005 at 428.141 kb on - strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.217 kb on + strand, within ABZR87_RS02005 at 428.218 kb on - strand, within ABZR87_RS02005 at 428.218 kb on - strand, within ABZR87_RS02005 at 428.218 kb on - strand, within ABZR87_RS02005 at 428.218 kb on - strand, within ABZR87_RS02005 at 428.218 kb on - strand, within ABZR87_RS02005 at 428.218 kb on - strand, within ABZR87_RS02005 at 428.307 kb on - strand, within ABZR87_RS02005 at 428.358 kb on + strand, within ABZR87_RS02005 at 428.358 kb on + strand, within ABZR87_RS02005 at 428.358 kb on + strand, within ABZR87_RS02005 at 428.358 kb on + strand, within ABZR87_RS02005 at 428.370 kb on + strand, within ABZR87_RS02005 at 428.370 kb on + strand, within ABZR87_RS02005 at 428.370 kb on + strand, within ABZR87_RS02005 at 428.370 kb on + strand, within ABZR87_RS02005 at 428.370 kb on + strand, within ABZR87_RS02005 at 428.370 kb on + strand, within ABZR87_RS02005 at 428.370 kb on + strand, within ABZR87_RS02005 at 428.370 kb on + strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.371 kb on - strand, within ABZR87_RS02005 at 428.372 kb on + strand, within ABZR87_RS02005 at 428.372 kb on + strand, within ABZR87_RS02005 at 428.373 kb on - strand, within ABZR87_RS02005 at 428.373 kb on - strand, within ABZR87_RS02005 at 428.373 kb on - strand, within ABZR87_RS02005 at 428.373 kb on - strand, within ABZR87_RS02005 at 428.397 kb on + strand, within ABZR87_RS02005 at 428.398 kb on - strand, within ABZR87_RS02005
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 4; outgrowth in LB remove 425,537 + ABZR87_RS01995 0.32 -0.9 425,537 + ABZR87_RS01995 0.32 +0.5 425,537 + ABZR87_RS01995 0.32 +0.3 425,537 + ABZR87_RS01995 0.32 +1.0 425,537 + ABZR87_RS01995 0.32 -0.4 425,538 - ABZR87_RS01995 0.32 -0.4 425,538 - ABZR87_RS01995 0.32 -0.3 425,538 - ABZR87_RS01995 0.32 -1.3 425,538 - ABZR87_RS01995 0.32 -1.1 425,538 - ABZR87_RS01995 0.32 -1.7 425,538 - ABZR87_RS01995 0.32 +0.1 425,582 + ABZR87_RS01995 0.36 -0.3 425,594 + ABZR87_RS01995 0.37 +1.3 425,594 + ABZR87_RS01995 0.37 +0.1 425,595 - ABZR87_RS01995 0.37 +0.6 425,595 - ABZR87_RS01995 0.37 -1.1 425,595 - ABZR87_RS01995 0.37 +0.5 425,595 - ABZR87_RS01995 0.37 +0.6 425,651 + ABZR87_RS01995 0.42 -0.2 425,651 + ABZR87_RS01995 0.42 -0.5 425,651 + ABZR87_RS01995 0.42 +0.3 425,651 + ABZR87_RS01995 0.42 -0.7 425,651 + ABZR87_RS01995 0.42 +1.0 425,652 - ABZR87_RS01995 0.42 -0.8 425,652 - ABZR87_RS01995 0.42 -1.5 425,753 + ABZR87_RS01995 0.50 +2.0 425,753 + ABZR87_RS01995 0.50 +1.7 425,754 - ABZR87_RS01995 0.50 +0.1 425,754 - ABZR87_RS01995 0.50 -0.0 425,780 - ABZR87_RS01995 0.53 +0.4 425,787 - ABZR87_RS01995 0.53 -1.0 425,843 + ABZR87_RS01995 0.58 +0.3 425,843 + ABZR87_RS01995 0.58 -0.7 425,844 - ABZR87_RS01995 0.58 +0.2 425,879 + ABZR87_RS01995 0.61 +0.0 425,879 + ABZR87_RS01995 0.61 -0.0 425,879 + ABZR87_RS01995 0.61 -0.6 425,880 - ABZR87_RS01995 0.61 +1.8 425,880 - ABZR87_RS01995 0.61 -0.5 425,880 - ABZR87_RS01995 0.61 -2.2 425,919 - ABZR87_RS01995 0.64 +1.0 425,981 + ABZR87_RS01995 0.70 +0.7 425,981 + ABZR87_RS01995 0.70 +0.4 425,982 - ABZR87_RS01995 0.70 -0.2 425,982 - ABZR87_RS01995 0.70 +0.3 425,982 - ABZR87_RS01995 0.70 +0.1 425,982 - ABZR87_RS01995 0.70 +0.8 425,982 - ABZR87_RS01995 0.70 +1.5 425,982 - ABZR87_RS01995 0.70 -0.6 425,984 + ABZR87_RS01995 0.70 -0.4 425,984 + ABZR87_RS01995 0.70 +1.3 425,984 + ABZR87_RS01995 0.70 +0.1 425,984 + ABZR87_RS01995 0.70 +0.9 425,985 - ABZR87_RS01995 0.70 -0.1 425,985 - ABZR87_RS01995 0.70 +1.2 425,985 - ABZR87_RS01995 0.70 +1.5 425,985 - ABZR87_RS01995 0.70 +0.2 426,002 + ABZR87_RS01995 0.72 +0.5 426,002 + ABZR87_RS01995 0.72 +0.6 426,002 + ABZR87_RS01995 0.72 -1.1 426,002 + ABZR87_RS01995 0.72 -0.6 426,003 - ABZR87_RS01995 0.72 +0.4 426,003 - ABZR87_RS01995 0.72 -0.9 426,003 - ABZR87_RS01995 0.72 +0.4 426,038 + ABZR87_RS01995 0.75 -0.7 426,038 + ABZR87_RS01995 0.75 -1.2 426,039 - ABZR87_RS01995 0.75 +0.5 426,041 + ABZR87_RS01995 0.75 +0.7 426,041 + ABZR87_RS01995 0.75 +1.1 426,041 + ABZR87_RS01995 0.75 +0.0 426,041 + ABZR87_RS01995 0.75 -0.7 426,041 + ABZR87_RS01995 0.75 -0.2 426,041 + ABZR87_RS01995 0.75 +0.3 426,042 - ABZR87_RS01995 0.75 -0.8 426,042 - ABZR87_RS01995 0.75 -0.5 426,042 - ABZR87_RS01995 0.75 -0.5 426,042 - ABZR87_RS01995 0.75 +0.4 426,042 - ABZR87_RS01995 0.75 +0.9 426,044 + ABZR87_RS01995 0.75 +1.7 426,045 - ABZR87_RS01995 0.75 -0.3 426,045 - ABZR87_RS01995 0.75 -0.2 426,125 + ABZR87_RS01995 0.82 -1.8 426,125 + ABZR87_RS01995 0.82 -0.5 426,126 - ABZR87_RS01995 0.82 -0.8 426,126 - ABZR87_RS01995 0.82 -0.5 426,152 + ABZR87_RS01995 0.84 +1.6 426,152 + ABZR87_RS01995 0.84 +0.8 426,152 + ABZR87_RS01995 0.84 -0.8 426,153 - ABZR87_RS01995 0.84 +0.2 426,153 - ABZR87_RS01995 0.84 -0.8 426,153 - ABZR87_RS01995 0.84 -0.0 426,153 - ABZR87_RS01995 0.84 +0.1 426,167 + ABZR87_RS01995 0.85 +1.2 426,206 + ABZR87_RS01995 0.89 +0.2 426,207 - ABZR87_RS01995 0.89 +0.2 426,207 - ABZR87_RS01995 0.89 +0.1 426,248 + -0.3 426,248 + -2.0 426,272 + -0.3 426,272 + +0.4 426,272 + +0.8 426,272 + -0.2 426,272 + -0.4 426,272 + -0.7 426,273 - -0.9 426,273 - -1.7 426,273 - +0.3 426,273 - -0.3 426,273 - -0.1 426,273 - +0.1 426,273 - +0.6 426,422 + +0.1 426,422 + -0.1 426,745 - ABZR87_RS02000 0.28 +1.0 426,943 - ABZR87_RS02000 0.47 -1.2 427,059 + ABZR87_RS02000 0.58 +0.3 427,059 + ABZR87_RS02000 0.58 -2.1 427,059 + ABZR87_RS02000 0.58 +0.3 427,059 + ABZR87_RS02000 0.58 +0.5 427,060 - ABZR87_RS02000 0.59 +0.6 427,248 + ABZR87_RS02000 0.77 -0.3 427,249 - ABZR87_RS02000 0.77 +0.6 427,249 - ABZR87_RS02000 0.77 +1.7 427,249 - ABZR87_RS02000 0.77 +0.7 427,249 - ABZR87_RS02000 0.77 -0.9 427,249 - ABZR87_RS02000 0.77 -0.2 427,537 + -0.1 427,538 - -1.5 427,538 - -0.0 427,622 + -0.2 427,622 + -0.5 427,622 + -0.7 427,622 + +0.5 427,683 + -1.8 427,683 + +0.5 427,684 - +0.2 427,684 - +0.2 427,691 - -1.0 427,692 + +2.5 427,692 + -0.5 427,692 + +0.9 427,692 + +0.6 427,692 + +0.2 427,692 + +0.3 427,692 + -0.0 427,692 + -0.5 427,692 + +1.2 427,692 + +0.8 427,693 - +1.1 427,693 - -1.0 427,693 - +1.0 427,693 - +1.2 427,693 - -0.2 427,693 - -2.5 427,698 + -0.5 427,698 + -1.1 427,698 + +0.3 427,698 + +0.9 427,698 + +0.8 427,699 - +1.2 427,699 - -0.3 427,699 - +1.1 427,699 - -0.3 427,699 - +0.3 427,699 - -0.8 427,699 - -1.3 427,800 - ABZR87_RS02005 0.17 -0.3 427,879 + ABZR87_RS02005 0.23 +0.5 427,880 - ABZR87_RS02005 0.23 +0.4 427,880 - ABZR87_RS02005 0.23 +0.0 427,880 - ABZR87_RS02005 0.23 -1.7 427,880 - ABZR87_RS02005 0.23 -0.4 427,880 - ABZR87_RS02005 0.23 +0.4 427,926 + ABZR87_RS02005 0.27 +1.6 427,926 + ABZR87_RS02005 0.27 +1.8 427,927 - ABZR87_RS02005 0.27 +1.7 428,140 + ABZR87_RS02005 0.43 +0.9 428,141 - ABZR87_RS02005 0.43 -1.2 428,217 + ABZR87_RS02005 0.49 +1.1 428,217 + ABZR87_RS02005 0.49 -0.9 428,217 + ABZR87_RS02005 0.49 +0.9 428,217 + ABZR87_RS02005 0.49 +1.0 428,217 + ABZR87_RS02005 0.49 +0.0 428,217 + ABZR87_RS02005 0.49 -0.1 428,217 + ABZR87_RS02005 0.49 -0.4 428,217 + ABZR87_RS02005 0.49 -0.2 428,217 + ABZR87_RS02005 0.49 -0.3 428,217 + ABZR87_RS02005 0.49 +0.3 428,218 - ABZR87_RS02005 0.49 +0.1 428,218 - ABZR87_RS02005 0.49 -0.0 428,218 - ABZR87_RS02005 0.49 -1.1 428,218 - ABZR87_RS02005 0.49 -0.3 428,218 - ABZR87_RS02005 0.49 -0.9 428,218 - ABZR87_RS02005 0.49 -0.6 428,307 - ABZR87_RS02005 0.56 +0.5 428,358 + ABZR87_RS02005 0.60 +0.0 428,358 + ABZR87_RS02005 0.60 +1.5 428,358 + ABZR87_RS02005 0.60 -0.5 428,358 + ABZR87_RS02005 0.60 +0.2 428,370 + ABZR87_RS02005 0.61 +0.4 428,370 + ABZR87_RS02005 0.61 +0.3 428,370 + ABZR87_RS02005 0.61 -0.8 428,370 + ABZR87_RS02005 0.61 +0.2 428,370 + ABZR87_RS02005 0.61 -0.0 428,370 + ABZR87_RS02005 0.61 -1.5 428,370 + ABZR87_RS02005 0.61 +0.7 428,370 + ABZR87_RS02005 0.61 +2.0 428,371 - ABZR87_RS02005 0.61 -0.4 428,371 - ABZR87_RS02005 0.61 -0.9 428,371 - ABZR87_RS02005 0.61 +0.6 428,371 - ABZR87_RS02005 0.61 +0.1 428,371 - ABZR87_RS02005 0.61 +1.1 428,371 - ABZR87_RS02005 0.61 +0.8 428,371 - ABZR87_RS02005 0.61 -0.3 428,371 - ABZR87_RS02005 0.61 -0.3 428,371 - ABZR87_RS02005 0.61 +0.8 428,371 - ABZR87_RS02005 0.61 -0.1 428,371 - ABZR87_RS02005 0.61 -0.1 428,371 - ABZR87_RS02005 0.61 -0.5 428,372 + ABZR87_RS02005 0.61 +2.5 428,372 + ABZR87_RS02005 0.61 +1.0 428,373 - ABZR87_RS02005 0.61 -0.4 428,373 - ABZR87_RS02005 0.61 -1.3 428,373 - ABZR87_RS02005 0.61 -0.9 428,373 - ABZR87_RS02005 0.61 +1.0 428,397 + ABZR87_RS02005 0.63 -1.3 428,398 - ABZR87_RS02005 0.63 -0.8
Or see this region's nucleotide sequence