Experiment: Chlorite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_181 and Pf1N1B4_182 overlap by 4 nucleotides Pf1N1B4_182 and Pf1N1B4_183 overlap by 4 nucleotides Pf1N1B4_183 and Pf1N1B4_184 are separated by 157 nucleotides
Pf1N1B4_181 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 224,292 to 225,233
_181
Pf1N1B4_182 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 225,230 to 225,985
_182
Pf1N1B4_183 - Major facilitator family transporter, at 225,982 to 227,283
_183
Pf1N1B4_184 - Chromosome initiation inhibitor, at 227,441 to 228,400
_184
Position (kb)
225
226
227
228 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 225.240 kb on + strand at 225.240 kb on + strand at 225.240 kb on + strand at 225.240 kb on + strand at 225.241 kb on - strand at 225.241 kb on - strand at 225.334 kb on + strand at 225.335 kb on - strand, within Pf1N1B4_182 at 225.558 kb on + strand at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand at 225.748 kb on - strand, within Pf1N1B4_182 at 225.748 kb on - strand, within Pf1N1B4_182 at 225.875 kb on - strand, within Pf1N1B4_182 at 225.980 kb on - strand at 225.980 kb on - strand at 225.989 kb on + strand at 226.236 kb on - strand, within Pf1N1B4_183 at 226.253 kb on + strand, within Pf1N1B4_183 at 226.494 kb on - strand, within Pf1N1B4_183 at 226.505 kb on + strand, within Pf1N1B4_183 at 226.505 kb on + strand, within Pf1N1B4_183 at 226.506 kb on - strand, within Pf1N1B4_183 at 226.506 kb on - strand, within Pf1N1B4_183 at 226.839 kb on - strand, within Pf1N1B4_183 at 226.839 kb on - strand at 226.960 kb on - strand, within Pf1N1B4_183 at 227.201 kb on + strand at 227.201 kb on + strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.202 kb on - strand at 227.208 kb on - strand at 227.217 kb on - strand at 227.275 kb on - strand at 227.298 kb on + strand at 227.298 kb on + strand at 227.298 kb on + strand at 227.298 kb on + strand at 227.298 kb on + strand at 227.299 kb on - strand at 227.299 kb on - strand at 227.299 kb on - strand at 227.299 kb on - strand at 227.299 kb on - strand at 227.302 kb on + strand at 227.302 kb on + strand at 227.302 kb on + strand at 227.303 kb on - strand at 227.305 kb on - strand at 227.318 kb on + strand at 227.358 kb on + strand at 227.358 kb on + strand at 227.359 kb on - strand at 227.359 kb on - strand at 227.364 kb on + strand at 227.409 kb on + strand at 227.412 kb on + strand at 227.427 kb on + strand at 227.440 kb on + strand at 227.456 kb on - strand at 227.516 kb on + strand at 227.516 kb on + strand at 227.517 kb on - strand at 227.517 kb on - strand at 227.807 kb on + strand, within Pf1N1B4_184 at 227.967 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.985 kb on - strand, within Pf1N1B4_184 at 227.991 kb on - strand, within Pf1N1B4_184 at 228.179 kb on + strand, within Pf1N1B4_184 at 228.179 kb on + strand, within Pf1N1B4_184 at 228.231 kb on + strand, within Pf1N1B4_184
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 1 mM remove 225,240 + +0.6 225,240 + -2.4 225,240 + -2.2 225,240 + -0.8 225,241 - -1.4 225,241 - -0.6 225,334 + +0.5 225,335 - Pf1N1B4_182 0.14 -0.2 225,558 + +2.4 225,558 + Pf1N1B4_182 0.43 +1.0 225,558 + Pf1N1B4_182 0.43 +2.1 225,558 + Pf1N1B4_182 0.43 -0.8 225,558 + Pf1N1B4_182 0.43 +0.6 225,558 + Pf1N1B4_182 0.43 +1.1 225,559 - Pf1N1B4_182 0.44 -1.8 225,559 - Pf1N1B4_182 0.44 +0.4 225,559 - +1.8 225,559 - Pf1N1B4_182 0.44 -0.5 225,559 - +0.8 225,748 - Pf1N1B4_182 0.69 +0.1 225,748 - Pf1N1B4_182 0.69 +0.8 225,875 - Pf1N1B4_182 0.85 -0.8 225,980 - -0.8 225,980 - +0.6 225,989 + -0.2 226,236 - Pf1N1B4_183 0.20 +0.8 226,253 + Pf1N1B4_183 0.21 +0.4 226,494 - Pf1N1B4_183 0.39 +0.8 226,505 + Pf1N1B4_183 0.40 -0.5 226,505 + Pf1N1B4_183 0.40 +0.5 226,506 - Pf1N1B4_183 0.40 -0.5 226,506 - Pf1N1B4_183 0.40 +0.8 226,839 - Pf1N1B4_183 0.66 +0.6 226,839 - +0.8 226,960 - Pf1N1B4_183 0.75 +0.8 227,201 + -1.5 227,201 + +0.2 227,202 - -2.0 227,202 - -0.6 227,202 - +0.2 227,208 - +0.0 227,217 - -2.2 227,275 - -0.2 227,298 + +0.0 227,298 + -0.2 227,298 + +0.6 227,298 + -0.7 227,298 + -0.1 227,299 - +1.8 227,299 - +1.2 227,299 - -1.9 227,299 - -1.9 227,299 - -0.8 227,302 + -0.5 227,302 + +0.3 227,302 + -3.2 227,303 - +1.1 227,305 - -0.2 227,318 + +1.0 227,358 + +0.5 227,358 + +0.2 227,359 - +0.8 227,359 - +0.2 227,364 + -0.2 227,409 + -0.4 227,412 + -1.0 227,427 + +0.0 227,440 + -1.2 227,456 - -2.2 227,516 + -0.3 227,516 + +0.2 227,517 - +0.0 227,517 - -1.2 227,807 + Pf1N1B4_184 0.38 -0.2 227,967 + Pf1N1B4_184 0.55 -0.4 227,984 + Pf1N1B4_184 0.57 -0.8 227,984 + Pf1N1B4_184 0.57 +0.2 227,984 + Pf1N1B4_184 0.57 +0.0 227,984 + Pf1N1B4_184 0.57 -1.0 227,985 - Pf1N1B4_184 0.57 +0.8 227,991 - Pf1N1B4_184 0.57 +0.5 228,179 + Pf1N1B4_184 0.77 -0.0 228,179 + Pf1N1B4_184 0.77 -1.0 228,231 + Pf1N1B4_184 0.82 -1.2
Or see this region's nucleotide sequence