Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_176

Experiment: Chlorite 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_175 and Pf1N1B4_176 are separated by 247 nucleotidesPf1N1B4_176 and Pf1N1B4_177 are separated by 151 nucleotides Pf1N1B4_175 - sigma-54 dependent transcriptional regulator, at 217,172 to 219,076 _175 Pf1N1B4_176 - Acetylpolyamine aminohydrolase, at 219,324 to 220,349 _176 Pf1N1B4_177 - N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87), at 220,501 to 221,730 _177 Position (kb) 219 220 221Strain fitness (log2 ratio) -2 -1 0 1 2at 218.388 kb on + strand, within Pf1N1B4_175at 218.563 kb on - strandat 218.608 kb on - strand, within Pf1N1B4_175at 218.608 kb on - strand, within Pf1N1B4_175at 218.981 kb on + strandat 218.981 kb on + strandat 219.044 kb on + strandat 219.044 kb on + strandat 219.045 kb on - strandat 219.045 kb on - strandat 219.045 kb on - strandat 219.069 kb on - strandat 219.169 kb on - strandat 219.169 kb on - strandat 219.306 kb on + strandat 219.307 kb on - strandat 219.507 kb on - strand, within Pf1N1B4_176at 219.718 kb on + strand, within Pf1N1B4_176at 219.719 kb on - strand, within Pf1N1B4_176at 219.719 kb on - strand, within Pf1N1B4_176at 219.719 kb on - strand, within Pf1N1B4_176at 219.847 kb on + strand, within Pf1N1B4_176at 219.847 kb on + strand, within Pf1N1B4_176at 219.848 kb on - strand, within Pf1N1B4_176at 219.908 kb on - strand, within Pf1N1B4_176at 220.021 kb on + strand, within Pf1N1B4_176at 220.022 kb on - strand, within Pf1N1B4_176at 220.153 kb on + strand, within Pf1N1B4_176at 220.277 kb on + strandat 220.298 kb on - strandat 220.298 kb on - strandat 220.298 kb on - strandat 220.300 kb on + strandat 220.300 kb on + strandat 220.300 kb on + strandat 220.300 kb on + strandat 220.300 kb on + strandat 220.300 kb on + strandat 220.301 kb on - strandat 220.301 kb on - strandat 220.301 kb on - strandat 220.358 kb on + strandat 220.358 kb on + strandat 220.374 kb on + strandat 220.374 kb on + strandat 220.374 kb on + strandat 220.374 kb on + strandat 220.374 kb on + strandat 220.375 kb on - strandat 220.503 kb on - strandat 220.782 kb on + strand, within Pf1N1B4_177at 220.783 kb on - strand, within Pf1N1B4_177at 220.783 kb on - strand, within Pf1N1B4_177at 220.929 kb on + strand, within Pf1N1B4_177at 220.930 kb on - strand, within Pf1N1B4_177at 220.930 kb on - strand, within Pf1N1B4_177at 220.930 kb on - strand, within Pf1N1B4_177at 220.930 kb on - strand, within Pf1N1B4_177at 221.053 kb on - strandat 221.053 kb on - strand, within Pf1N1B4_177at 221.053 kb on - strand, within Pf1N1B4_177at 221.183 kb on + strand, within Pf1N1B4_177at 221.184 kb on - strandat 221.184 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 1 mM
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218,388 + Pf1N1B4_175 0.64 -1.7
218,563 - -1.2
218,608 - Pf1N1B4_175 0.75 -0.6
218,608 - Pf1N1B4_175 0.75 -0.8
218,981 + -0.3
218,981 + -0.5
219,044 + +0.2
219,044 + +0.8
219,045 - -0.5
219,045 - -1.0
219,045 - +0.5
219,069 - -0.6
219,169 - -1.8
219,169 - -0.4
219,306 + +0.2
219,307 - +0.0
219,507 - Pf1N1B4_176 0.18 +0.0
219,718 + Pf1N1B4_176 0.38 -1.4
219,719 - Pf1N1B4_176 0.38 +0.5
219,719 - Pf1N1B4_176 0.38 +0.0
219,719 - Pf1N1B4_176 0.38 +1.1
219,847 + Pf1N1B4_176 0.51 +0.0
219,847 + Pf1N1B4_176 0.51 +1.5
219,848 - Pf1N1B4_176 0.51 -0.2
219,908 - Pf1N1B4_176 0.57 -0.1
220,021 + Pf1N1B4_176 0.68 +2.4
220,022 - Pf1N1B4_176 0.68 +0.5
220,153 + Pf1N1B4_176 0.81 +0.8
220,277 + -0.8
220,298 - +1.0
220,298 - +1.4
220,298 - +0.8
220,300 + +0.3
220,300 + -0.1
220,300 + +1.8
220,300 + -0.1
220,300 + -0.8
220,300 + +2.4
220,301 - +0.8
220,301 - +0.2
220,301 - -0.4
220,358 + -0.3
220,358 + -0.5
220,374 + +0.8
220,374 + +0.1
220,374 + -1.2
220,374 + +1.1
220,374 + -1.6
220,375 - +0.3
220,503 - -0.1
220,782 + Pf1N1B4_177 0.23 -0.1
220,783 - Pf1N1B4_177 0.23 +0.2
220,783 - Pf1N1B4_177 0.23 -1.2
220,929 + Pf1N1B4_177 0.35 -0.8
220,930 - Pf1N1B4_177 0.35 +0.8
220,930 - Pf1N1B4_177 0.35 -0.7
220,930 - Pf1N1B4_177 0.35 -1.0
220,930 - Pf1N1B4_177 0.35 +0.1
221,053 - +1.4
221,053 - Pf1N1B4_177 0.45 -2.0
221,053 - Pf1N1B4_177 0.45 -0.4
221,183 + Pf1N1B4_177 0.55 +1.0
221,184 - +1.4
221,184 - +0.8

Or see this region's nucleotide sequence