Experiment: Chlorite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1486 and Pf1N1B4_1487 are separated by 375 nucleotides Pf1N1B4_1487 and Pf1N1B4_1488 are separated by 122 nucleotides Pf1N1B4_1488 and Pf1N1B4_1489 are separated by 12 nucleotides
Pf1N1B4_1486 - VgrG protein, at 1,711,402 to 1,713,420
_1486
Pf1N1B4_1487 - Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter, at 1,713,796 to 1,715,280
_1487
Pf1N1B4_1488 - hypothetical protein, at 1,715,403 to 1,715,729
_1488
Pf1N1B4_1489 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12), at 1,715,742 to 1,719,695
_1489
Position (kb)
1713
1714
1715
1716 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1712.975 kb on + strand, within Pf1N1B4_1486 at 1713.087 kb on + strand, within Pf1N1B4_1486 at 1713.087 kb on + strand, within Pf1N1B4_1486 at 1713.111 kb on + strand, within Pf1N1B4_1486 at 1713.125 kb on + strand, within Pf1N1B4_1486 at 1713.125 kb on + strand, within Pf1N1B4_1486 at 1713.125 kb on + strand, within Pf1N1B4_1486 at 1713.125 kb on + strand, within Pf1N1B4_1486 at 1713.352 kb on + strand at 1713.353 kb on - strand at 1713.353 kb on - strand at 1713.353 kb on - strand at 1713.353 kb on - strand at 1713.353 kb on - strand at 1713.502 kb on + strand at 1713.509 kb on - strand at 1713.552 kb on - strand at 1713.627 kb on + strand at 1713.627 kb on + strand at 1713.627 kb on + strand at 1713.627 kb on + strand at 1713.627 kb on + strand at 1713.628 kb on - strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.629 kb on + strand at 1713.630 kb on - strand at 1713.630 kb on - strand at 1713.711 kb on - strand at 1713.763 kb on + strand at 1713.764 kb on - strand at 1713.882 kb on - strand at 1713.882 kb on - strand at 1713.882 kb on - strand at 1713.882 kb on - strand at 1714.074 kb on - strand, within Pf1N1B4_1487 at 1714.074 kb on - strand, within Pf1N1B4_1487 at 1714.091 kb on - strand, within Pf1N1B4_1487 at 1714.208 kb on + strand at 1714.209 kb on - strand, within Pf1N1B4_1487 at 1714.209 kb on - strand, within Pf1N1B4_1487 at 1714.294 kb on + strand, within Pf1N1B4_1487 at 1714.397 kb on + strand, within Pf1N1B4_1487 at 1714.398 kb on - strand, within Pf1N1B4_1487 at 1714.398 kb on - strand, within Pf1N1B4_1487 at 1714.398 kb on - strand, within Pf1N1B4_1487 at 1714.398 kb on - strand, within Pf1N1B4_1487 at 1714.398 kb on - strand, within Pf1N1B4_1487 at 1714.714 kb on + strand, within Pf1N1B4_1487 at 1714.715 kb on - strand, within Pf1N1B4_1487 at 1714.760 kb on + strand, within Pf1N1B4_1487 at 1714.803 kb on - strand, within Pf1N1B4_1487 at 1714.862 kb on + strand, within Pf1N1B4_1487 at 1714.862 kb on + strand, within Pf1N1B4_1487 at 1714.862 kb on + strand, within Pf1N1B4_1487 at 1714.943 kb on + strand, within Pf1N1B4_1487 at 1715.021 kb on + strand, within Pf1N1B4_1487 at 1715.022 kb on - strand, within Pf1N1B4_1487 at 1715.022 kb on - strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.075 kb on + strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand, within Pf1N1B4_1487 at 1715.076 kb on - strand at 1715.178 kb on - strand at 1715.241 kb on - strand at 1715.241 kb on - strand at 1715.241 kb on - strand at 1715.305 kb on + strand at 1715.305 kb on + strand at 1715.306 kb on - strand at 1715.306 kb on - strand at 1715.310 kb on - strand at 1715.310 kb on - strand at 1715.310 kb on - strand at 1715.375 kb on + strand at 1715.376 kb on - strand at 1715.376 kb on - strand at 1715.469 kb on + strand, within Pf1N1B4_1488 at 1715.495 kb on - strand, within Pf1N1B4_1488 at 1715.506 kb on - strand, within Pf1N1B4_1488 at 1715.568 kb on + strand, within Pf1N1B4_1488 at 1715.574 kb on + strand, within Pf1N1B4_1488 at 1715.575 kb on - strand, within Pf1N1B4_1488 at 1715.575 kb on - strand, within Pf1N1B4_1488 at 1715.575 kb on - strand, within Pf1N1B4_1488 at 1715.602 kb on + strand, within Pf1N1B4_1488 at 1715.689 kb on - strand, within Pf1N1B4_1488 at 1715.689 kb on - strand, within Pf1N1B4_1488 at 1715.741 kb on + strand at 1715.742 kb on - strand at 1715.742 kb on - strand at 1715.742 kb on - strand at 1716.223 kb on + strand, within Pf1N1B4_1489 at 1716.223 kb on + strand, within Pf1N1B4_1489
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 1 mM remove 1,712,975 + Pf1N1B4_1486 0.78 +2.9 1,713,087 + Pf1N1B4_1486 0.83 -0.4 1,713,087 + Pf1N1B4_1486 0.83 +0.0 1,713,111 + Pf1N1B4_1486 0.85 +0.4 1,713,125 + Pf1N1B4_1486 0.85 -0.5 1,713,125 + Pf1N1B4_1486 0.85 +0.2 1,713,125 + Pf1N1B4_1486 0.85 +1.8 1,713,125 + Pf1N1B4_1486 0.85 +0.0 1,713,352 + +0.6 1,713,353 - +2.4 1,713,353 - +0.4 1,713,353 - -1.0 1,713,353 - -0.4 1,713,353 - +3.4 1,713,502 + -0.5 1,713,509 - +1.4 1,713,552 - -1.4 1,713,627 + -1.0 1,713,627 + -1.4 1,713,627 + -0.8 1,713,627 + +0.9 1,713,627 + +0.1 1,713,628 - -0.3 1,713,629 + +0.9 1,713,629 + -1.0 1,713,629 + +0.4 1,713,629 + -0.6 1,713,629 + -0.2 1,713,629 + -1.4 1,713,629 + -0.2 1,713,629 + +0.0 1,713,629 + -0.4 1,713,629 + -0.7 1,713,629 + -0.2 1,713,629 + -0.2 1,713,630 - -1.0 1,713,630 - +1.2 1,713,711 - +0.9 1,713,763 + +1.4 1,713,764 - -2.1 1,713,882 - +0.3 1,713,882 - -1.1 1,713,882 - -0.4 1,713,882 - -0.6 1,714,074 - Pf1N1B4_1487 0.19 +0.1 1,714,074 - Pf1N1B4_1487 0.19 -0.3 1,714,091 - Pf1N1B4_1487 0.20 +1.1 1,714,208 + +1.4 1,714,209 - Pf1N1B4_1487 0.28 +0.7 1,714,209 - Pf1N1B4_1487 0.28 -0.6 1,714,294 + Pf1N1B4_1487 0.34 -1.1 1,714,397 + Pf1N1B4_1487 0.40 +1.4 1,714,398 - Pf1N1B4_1487 0.41 -0.6 1,714,398 - Pf1N1B4_1487 0.41 -0.8 1,714,398 - Pf1N1B4_1487 0.41 +0.8 1,714,398 - Pf1N1B4_1487 0.41 +0.4 1,714,398 - Pf1N1B4_1487 0.41 +1.1 1,714,714 + Pf1N1B4_1487 0.62 +1.8 1,714,715 - Pf1N1B4_1487 0.62 +0.4 1,714,760 + Pf1N1B4_1487 0.65 +0.1 1,714,803 - Pf1N1B4_1487 0.68 +0.4 1,714,862 + Pf1N1B4_1487 0.72 +1.2 1,714,862 + Pf1N1B4_1487 0.72 -0.8 1,714,862 + Pf1N1B4_1487 0.72 +1.6 1,714,943 + Pf1N1B4_1487 0.77 +0.6 1,715,021 + Pf1N1B4_1487 0.82 +0.9 1,715,022 - Pf1N1B4_1487 0.83 -0.0 1,715,022 - Pf1N1B4_1487 0.83 -0.2 1,715,075 + Pf1N1B4_1487 0.86 +0.7 1,715,075 + Pf1N1B4_1487 0.86 -0.1 1,715,075 + Pf1N1B4_1487 0.86 -1.4 1,715,075 + Pf1N1B4_1487 0.86 +0.5 1,715,075 + Pf1N1B4_1487 0.86 +0.4 1,715,075 + Pf1N1B4_1487 0.86 +0.4 1,715,075 + Pf1N1B4_1487 0.86 -1.8 1,715,075 + Pf1N1B4_1487 0.86 +1.6 1,715,076 - Pf1N1B4_1487 0.86 +1.0 1,715,076 - Pf1N1B4_1487 0.86 -1.0 1,715,076 - Pf1N1B4_1487 0.86 +0.2 1,715,076 - Pf1N1B4_1487 0.86 -0.6 1,715,076 - Pf1N1B4_1487 0.86 -1.0 1,715,076 - Pf1N1B4_1487 0.86 +0.6 1,715,076 - +2.7 1,715,178 - -1.0 1,715,241 - -1.0 1,715,241 - +0.3 1,715,241 - -0.2 1,715,305 + -0.4 1,715,305 + -0.4 1,715,306 - +0.6 1,715,306 - -0.2 1,715,310 - -1.2 1,715,310 - +0.6 1,715,310 - -0.4 1,715,375 + -0.1 1,715,376 - -1.0 1,715,376 - +0.4 1,715,469 + Pf1N1B4_1488 0.20 +0.6 1,715,495 - Pf1N1B4_1488 0.28 -0.1 1,715,506 - Pf1N1B4_1488 0.31 -0.6 1,715,568 + Pf1N1B4_1488 0.50 -1.6 1,715,574 + Pf1N1B4_1488 0.52 -0.9 1,715,575 - Pf1N1B4_1488 0.53 +1.2 1,715,575 - Pf1N1B4_1488 0.53 -0.0 1,715,575 - Pf1N1B4_1488 0.53 -0.4 1,715,602 + Pf1N1B4_1488 0.61 -0.2 1,715,689 - Pf1N1B4_1488 0.87 -1.2 1,715,689 - Pf1N1B4_1488 0.87 +0.1 1,715,741 + -0.2 1,715,742 - -0.5 1,715,742 - -1.0 1,715,742 - +0.1 1,716,223 + Pf1N1B4_1489 0.12 +0.9 1,716,223 + Pf1N1B4_1489 0.12 +0.6
Or see this region's nucleotide sequence