Experiment: Chloramphenicol 0.0006640625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnd and tRNA-Val are separated by 38 nucleotides tRNA-Val and rbp1 are separated by 272 nucleotides rbp1 and Synpcc7942_0680 are separated by 225 nucleotides Synpcc7942_0680 and ispD are separated by 14 nucleotides ispD and Synpcc7942_0682 are separated by 83 nucleotides
Synpcc7942_0678: rnd - 3'-5' exonuclease, at 672,767 to 673,402
rnd
Synpcc7942_R0038: tRNA-Val - tRNA-Val, at 673,441 to 673,512
tRNA-Val
Synpcc7942_0679: rbp1 - RNA-binding region RNP-1, at 673,785 to 674,108
rbp1
Synpcc7942_0680: Synpcc7942_0680 - C-5 sterol desaturase, at 674,334 to 675,149
_0680
Synpcc7942_0681: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 675,164 to 675,862
ispD
Synpcc7942_0682: Synpcc7942_0682 - hypothetical protein, at 675,946 to 676,905
_0682
Position (kb) 674
675
676 Strain fitness (log2 ratio) -2
-1
0
1
2 at 673.361 kb on - strand at 673.378 kb on + strand at 673.394 kb on - strand at 673.396 kb on + strand at 673.399 kb on + strand at 673.410 kb on - strand at 673.443 kb on - strand at 673.581 kb on + strand at 673.633 kb on - strand at 673.651 kb on + strand at 673.811 kb on - strand at 673.861 kb on - strand, within rbp1 at 673.862 kb on + strand, within rbp1 at 673.870 kb on - strand, within rbp1 at 673.875 kb on - strand, within rbp1 at 673.952 kb on - strand, within rbp1 at 673.975 kb on + strand, within rbp1 at 673.978 kb on - strand, within rbp1 at 674.020 kb on + strand, within rbp1 at 674.041 kb on - strand, within rbp1 at 674.116 kb on + strand at 674.124 kb on - strand at 674.125 kb on - strand at 674.184 kb on + strand at 674.535 kb on + strand, within Synpcc7942_0680 at 674.537 kb on - strand, within Synpcc7942_0680 at 674.610 kb on - strand, within Synpcc7942_0680 at 674.771 kb on - strand, within Synpcc7942_0680 at 674.792 kb on - strand, within Synpcc7942_0680 at 674.801 kb on + strand, within Synpcc7942_0680 at 674.886 kb on - strand, within Synpcc7942_0680 at 674.886 kb on - strand, within Synpcc7942_0680 at 674.922 kb on - strand, within Synpcc7942_0680 at 674.923 kb on + strand, within Synpcc7942_0680 at 674.924 kb on - strand, within Synpcc7942_0680 at 674.959 kb on - strand, within Synpcc7942_0680 at 674.960 kb on - strand, within Synpcc7942_0680 at 674.975 kb on + strand, within Synpcc7942_0680 at 675.004 kb on - strand, within Synpcc7942_0680 at 675.119 kb on + strand at 675.152 kb on - strand at 675.164 kb on - strand at 675.191 kb on - strand at 675.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0006640625 mg/ml remove 673,361 - -2.7 673,378 + +0.9 673,394 - +0.5 673,396 + +1.3 673,399 + +0.5 673,410 - -1.1 673,443 - +0.7 673,581 + +1.7 673,633 - -1.1 673,651 + -2.1 673,811 - -0.1 673,861 - rbp1 Synpcc7942_0679 0.23 +0.9 673,862 + rbp1 Synpcc7942_0679 0.24 -1.1 673,870 - rbp1 Synpcc7942_0679 0.26 -2.1 673,875 - rbp1 Synpcc7942_0679 0.28 -0.1 673,952 - rbp1 Synpcc7942_0679 0.52 -1.1 673,975 + rbp1 Synpcc7942_0679 0.59 +0.9 673,978 - rbp1 Synpcc7942_0679 0.60 +0.5 674,020 + rbp1 Synpcc7942_0679 0.73 -0.8 674,041 - rbp1 Synpcc7942_0679 0.79 -1.4 674,116 + +0.5 674,124 - +1.5 674,125 - -1.7 674,184 + -1.1 674,535 + Synpcc7942_0680 0.25 -0.7 674,537 - Synpcc7942_0680 0.25 -0.5 674,610 - Synpcc7942_0680 0.34 +0.9 674,771 - Synpcc7942_0680 0.54 -1.4 674,792 - Synpcc7942_0680 0.56 +0.9 674,801 + Synpcc7942_0680 0.57 -1.4 674,886 - Synpcc7942_0680 0.68 -1.7 674,886 - Synpcc7942_0680 0.68 -0.8 674,922 - Synpcc7942_0680 0.72 -1.1 674,923 + Synpcc7942_0680 0.72 +1.9 674,924 - Synpcc7942_0680 0.72 -2.4 674,959 - Synpcc7942_0680 0.77 -0.1 674,960 - Synpcc7942_0680 0.77 -1.5 674,975 + Synpcc7942_0680 0.79 -1.1 675,004 - Synpcc7942_0680 0.82 +0.2 675,119 + -2.1 675,152 - +0.9 675,164 - +0.3 675,191 - +0.9 675,948 - -0.5
Or see this region's nucleotide sequence