Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1967 and SMa1968 are separated by 194 nucleotides SMa1968 and SMa1969 are separated by 76 nucleotides SMa1969 and SMa1971 are separated by 129 nucleotides SMa1971 and SMa1973 are separated by 13 nucleotides SMa1973 and SMa1974 overlap by 1 nucleotides
SMa1967: SMa1967 - short chain alcohol, at 1,119,543 to 1,119,881
SMa1967
SMa1968: SMa1968 - amidotransferase, at 1,120,076 to 1,120,768
SMa1968
SMa1969: SMa1969 - amidotransferase, at 1,120,845 to 1,121,684
SMa1969
SMa1971: SMa1971 - hypothetical protein, at 1,121,814 to 1,122,110
SMa1971
SMa1973: SMa1973 - hypothetical protein, at 1,122,124 to 1,122,483
SMa1973
SMa1974: SMa1974 - hypothetical protein, at 1,122,483 to 1,123,199
SMa1974
Position (kb)
1120
1121
1122 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1119.945 kb on + strand at 1119.945 kb on + strand at 1119.946 kb on - strand at 1119.948 kb on + strand at 1119.949 kb on - strand at 1119.949 kb on - strand at 1120.023 kb on + strand at 1120.154 kb on + strand, within SMa1968 at 1120.155 kb on - strand, within SMa1968 at 1120.166 kb on + strand, within SMa1968 at 1120.167 kb on - strand, within SMa1968 at 1120.403 kb on - strand, within SMa1968 at 1120.403 kb on - strand, within SMa1968 at 1120.403 kb on - strand, within SMa1968 at 1120.403 kb on - strand, within SMa1968 at 1120.454 kb on + strand, within SMa1968 at 1120.455 kb on - strand, within SMa1968 at 1121.104 kb on - strand, within SMa1969 at 1121.242 kb on - strand, within SMa1969 at 1121.302 kb on + strand, within SMa1969 at 1121.303 kb on - strand, within SMa1969 at 1121.445 kb on + strand, within SMa1969 at 1121.445 kb on + strand, within SMa1969 at 1121.445 kb on + strand, within SMa1969 at 1121.445 kb on + strand, within SMa1969 at 1121.446 kb on - strand, within SMa1969 at 1121.446 kb on - strand, within SMa1969 at 1121.446 kb on - strand, within SMa1969 at 1121.454 kb on + strand, within SMa1969 at 1121.682 kb on + strand at 1121.683 kb on - strand at 1121.756 kb on + strand at 1121.756 kb on + strand at 1121.823 kb on - strand at 1121.823 kb on - strand at 1121.827 kb on + strand at 1121.828 kb on - strand at 1121.883 kb on + strand, within SMa1971 at 1121.884 kb on - strand, within SMa1971 at 1121.884 kb on - strand, within SMa1971 at 1121.934 kb on + strand, within SMa1971 at 1121.934 kb on + strand, within SMa1971 at 1121.934 kb on + strand, within SMa1971 at 1121.934 kb on + strand, within SMa1971 at 1121.935 kb on - strand, within SMa1971 at 1121.970 kb on + strand, within SMa1971 at 1121.970 kb on + strand, within SMa1971 at 1122.005 kb on - strand, within SMa1971 at 1122.135 kb on + strand at 1122.136 kb on - strand at 1122.169 kb on - strand, within SMa1973 at 1122.219 kb on - strand, within SMa1973 at 1122.337 kb on + strand, within SMa1973 at 1122.337 kb on + strand, within SMa1973 at 1122.338 kb on - strand, within SMa1973 at 1122.442 kb on - strand, within SMa1973 at 1122.575 kb on + strand, within SMa1974 at 1122.575 kb on + strand, within SMa1974 at 1122.575 kb on + strand, within SMa1974 at 1122.575 kb on + strand, within SMa1974 at 1122.575 kb on + strand, within SMa1974 at 1122.576 kb on - strand, within SMa1974 at 1122.576 kb on - strand, within SMa1974 at 1122.576 kb on - strand, within SMa1974 at 1122.576 kb on - strand, within SMa1974 at 1122.583 kb on - strand, within SMa1974
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 1,119,945 + -1.7 1,119,945 + +0.0 1,119,946 - -2.7 1,119,948 + +0.2 1,119,949 - +0.3 1,119,949 - -0.5 1,120,023 + -0.8 1,120,154 + SMa1968 0.11 -3.1 1,120,155 - SMa1968 0.11 +0.3 1,120,166 + SMa1968 0.13 +1.2 1,120,167 - SMa1968 0.13 -1.9 1,120,403 - SMa1968 0.47 -1.0 1,120,403 - SMa1968 0.47 -0.1 1,120,403 - SMa1968 0.47 -0.4 1,120,403 - SMa1968 0.47 -0.6 1,120,454 + SMa1968 0.55 -0.6 1,120,455 - SMa1968 0.55 +0.5 1,121,104 - SMa1969 0.31 -0.2 1,121,242 - SMa1969 0.47 +0.9 1,121,302 + SMa1969 0.54 -0.3 1,121,303 - SMa1969 0.55 +0.4 1,121,445 + SMa1969 0.71 -1.3 1,121,445 + SMa1969 0.71 +0.2 1,121,445 + SMa1969 0.71 -0.7 1,121,445 + SMa1969 0.71 -0.5 1,121,446 - SMa1969 0.72 -0.4 1,121,446 - SMa1969 0.72 -3.2 1,121,446 - SMa1969 0.72 -1.1 1,121,454 + SMa1969 0.72 -0.2 1,121,682 + +1.8 1,121,683 - +0.9 1,121,756 + +0.3 1,121,756 + -0.5 1,121,823 - -1.9 1,121,823 - -0.2 1,121,827 + -0.7 1,121,828 - +0.8 1,121,883 + SMa1971 0.23 +0.2 1,121,884 - SMa1971 0.24 -2.1 1,121,884 - SMa1971 0.24 +0.7 1,121,934 + SMa1971 0.40 -0.3 1,121,934 + SMa1971 0.40 -2.0 1,121,934 + SMa1971 0.40 +0.3 1,121,934 + SMa1971 0.40 +0.6 1,121,935 - SMa1971 0.41 +0.2 1,121,970 + SMa1971 0.53 +1.6 1,121,970 + SMa1971 0.53 +0.4 1,122,005 - SMa1971 0.64 +0.2 1,122,135 + +0.4 1,122,136 - -0.5 1,122,169 - SMa1973 0.12 +0.8 1,122,219 - SMa1973 0.26 -0.3 1,122,337 + SMa1973 0.59 +1.0 1,122,337 + SMa1973 0.59 -3.9 1,122,338 - SMa1973 0.59 +1.0 1,122,442 - SMa1973 0.88 +0.4 1,122,575 + SMa1974 0.13 -1.0 1,122,575 + SMa1974 0.13 +0.4 1,122,575 + SMa1974 0.13 +0.5 1,122,575 + SMa1974 0.13 -1.2 1,122,575 + SMa1974 0.13 -0.0 1,122,576 - SMa1974 0.13 -0.3 1,122,576 - SMa1974 0.13 -0.4 1,122,576 - SMa1974 0.13 +0.2 1,122,576 - SMa1974 0.13 +0.1 1,122,583 - SMa1974 0.14 -0.4
Or see this region's nucleotide sequence