Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0126 and SMa0128 are separated by 120 nucleotides SMa0128 and SMa0130 are separated by 85 nucleotides SMa0130 and SMa0132 overlap by 1 nucleotides SMa0132 and SMa0134 are separated by 110 nucleotides
SMa0126: SMa0126 - CspA8 Cold shock family protein, at 68,805 to 69,014
SMa0126
SMa0128: SMa0128 - hypothetical protein, at 69,135 to 69,515
SMa0128
SMa0130: SMa0130 - fatty acid desaturase, at 69,601 to 70,668
SMa0130
SMa0132: SMa0132 - hypothetical protein, at 70,668 to 70,955
SMa0132
SMa0134: SMa0134 - hypothetical protein, at 71,066 to 71,671
SMa0134
Position (kb) 69
70
71 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 68.678 kb on + strand at 68.679 kb on - strand at 68.694 kb on - strand at 68.781 kb on + strand at 68.781 kb on + strand at 68.782 kb on - strand at 68.804 kb on + strand at 68.822 kb on + strand at 68.822 kb on + strand at 68.823 kb on - strand at 68.823 kb on - strand at 68.972 kb on + strand, within SMa0126 at 68.972 kb on + strand, within SMa0126 at 69.044 kb on + strand at 69.044 kb on + strand at 69.044 kb on + strand at 69.104 kb on + strand at 69.130 kb on - strand at 69.130 kb on - strand at 69.279 kb on - strand, within SMa0128 at 69.513 kb on + strand at 69.513 kb on + strand at 69.513 kb on + strand at 69.597 kb on + strand at 69.598 kb on - strand at 69.600 kb on - strand at 69.616 kb on + strand at 69.616 kb on + strand at 69.617 kb on - strand at 69.617 kb on - strand at 69.617 kb on - strand at 69.676 kb on + strand at 70.030 kb on + strand, within SMa0130 at 70.072 kb on + strand, within SMa0130 at 70.309 kb on + strand, within SMa0130 at 70.309 kb on + strand, within SMa0130 at 70.396 kb on + strand, within SMa0130 at 70.397 kb on - strand, within SMa0130 at 70.397 kb on - strand, within SMa0130 at 70.435 kb on - strand, within SMa0130 at 70.477 kb on + strand, within SMa0130 at 70.497 kb on - strand, within SMa0130 at 70.598 kb on + strand at 70.666 kb on + strand at 70.776 kb on + strand, within SMa0132 at 70.777 kb on - strand, within SMa0132 at 70.777 kb on - strand, within SMa0132 at 70.880 kb on + strand, within SMa0132 at 70.936 kb on + strand at 70.937 kb on - strand at 70.953 kb on + strand at 70.954 kb on - strand at 70.954 kb on - strand at 71.166 kb on + strand, within SMa0134 at 71.166 kb on + strand, within SMa0134 at 71.166 kb on + strand, within SMa0134 at 71.166 kb on + strand, within SMa0134 at 71.166 kb on + strand, within SMa0134 at 71.167 kb on - strand, within SMa0134 at 71.167 kb on - strand, within SMa0134 at 71.167 kb on - strand, within SMa0134 at 71.292 kb on + strand, within SMa0134 at 71.293 kb on - strand, within SMa0134 at 71.420 kb on + strand, within SMa0134 at 71.420 kb on + strand, within SMa0134 at 71.496 kb on - strand, within SMa0134 at 71.496 kb on - strand, within SMa0134 at 71.640 kb on + strand at 71.660 kb on + strand at 71.660 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 68,678 + +0.4 68,679 - +0.4 68,694 - +0.5 68,781 + -0.2 68,781 + +0.5 68,782 - +0.4 68,804 + -0.2 68,822 + +4.5 68,822 + +0.4 68,823 - +1.9 68,823 - -2.3 68,972 + SMa0126 0.80 -0.6 68,972 + SMa0126 0.80 -1.5 69,044 + -0.8 69,044 + -0.4 69,044 + +1.0 69,104 + -0.6 69,130 - +0.5 69,130 - -0.8 69,279 - SMa0128 0.38 +1.7 69,513 + +0.8 69,513 + -0.1 69,513 + +0.0 69,597 + -1.2 69,598 - -3.4 69,600 - +0.1 69,616 + +0.9 69,616 + +0.9 69,617 - +1.5 69,617 - -0.8 69,617 - +4.6 69,676 + -0.8 70,030 + SMa0130 0.40 -4.8 70,072 + SMa0130 0.44 -3.0 70,309 + SMa0130 0.66 -1.8 70,309 + SMa0130 0.66 +1.1 70,396 + SMa0130 0.74 -0.2 70,397 - SMa0130 0.75 -0.2 70,397 - SMa0130 0.75 -2.0 70,435 - SMa0130 0.78 +0.2 70,477 + SMa0130 0.82 -0.9 70,497 - SMa0130 0.84 +0.9 70,598 + +1.9 70,666 + -2.4 70,776 + SMa0132 0.38 +0.2 70,777 - SMa0132 0.38 -0.1 70,777 - SMa0132 0.38 -1.3 70,880 + SMa0132 0.74 +2.3 70,936 + -0.8 70,937 - -0.8 70,953 + -0.1 70,954 - +2.9 70,954 - -0.0 71,166 + SMa0134 0.17 +0.1 71,166 + SMa0134 0.17 +0.0 71,166 + SMa0134 0.17 +0.6 71,166 + SMa0134 0.17 -0.8 71,166 + SMa0134 0.17 -0.9 71,167 - SMa0134 0.17 -2.4 71,167 - SMa0134 0.17 -0.2 71,167 - SMa0134 0.17 +1.1 71,292 + SMa0134 0.37 -0.3 71,293 - SMa0134 0.37 +0.4 71,420 + SMa0134 0.58 +0.7 71,420 + SMa0134 0.58 +1.1 71,496 - SMa0134 0.71 -1.3 71,496 - SMa0134 0.71 -0.7 71,640 + +1.4 71,660 + -1.3 71,660 + +0.7
Or see this region's nucleotide sequence