Experiment: PYE with Chloramphenicol 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02190 and CCNA_02191 are separated by 80 nucleotides CCNA_02191 and CCNA_02192 overlap by 33 nucleotides CCNA_02192 and CCNA_02193 overlap by 43 nucleotides CCNA_02193 and CCNA_02194 are separated by 124 nucleotides
CCNA_02190: CCNA_02190 - penicillin-binding protein, at 2,340,908 to 2,343,046
_02190
CCNA_02191: CCNA_02191 - hypothetical protein, at 2,343,127 to 2,343,513
_02191
CCNA_02192: CCNA_02192 - endodeoxyribonuclease RusA, at 2,343,481 to 2,343,921
_02192
CCNA_02193: CCNA_02193 - hypothetical protein, at 2,343,879 to 2,344,412
_02193
CCNA_02194: CCNA_02194 - fumarate hydratase, at 2,344,537 to 2,345,928
_02194
Position (kb)
2343
2344 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2342.481 kb on + strand, within CCNA_02190 at 2342.487 kb on - strand, within CCNA_02190 at 2342.489 kb on - strand, within CCNA_02190 at 2342.489 kb on - strand, within CCNA_02190 at 2342.491 kb on + strand, within CCNA_02190 at 2342.491 kb on + strand, within CCNA_02190 at 2342.499 kb on - strand, within CCNA_02190 at 2342.499 kb on - strand, within CCNA_02190 at 2342.554 kb on + strand, within CCNA_02190 at 2342.562 kb on - strand, within CCNA_02190 at 2342.572 kb on - strand, within CCNA_02190 at 2342.620 kb on + strand, within CCNA_02190 at 2342.628 kb on - strand, within CCNA_02190 at 2342.647 kb on + strand, within CCNA_02190 at 2342.650 kb on - strand, within CCNA_02190 at 2342.660 kb on - strand, within CCNA_02190 at 2342.673 kb on - strand, within CCNA_02190 at 2342.709 kb on - strand, within CCNA_02190 at 2342.709 kb on - strand, within CCNA_02190 at 2342.711 kb on + strand, within CCNA_02190 at 2342.724 kb on - strand, within CCNA_02190 at 2342.726 kb on + strand, within CCNA_02190 at 2342.734 kb on - strand, within CCNA_02190 at 2342.734 kb on - strand, within CCNA_02190 at 2342.755 kb on - strand, within CCNA_02190 at 2342.775 kb on + strand, within CCNA_02190 at 2342.804 kb on + strand, within CCNA_02190 at 2342.812 kb on - strand, within CCNA_02190 at 2342.812 kb on - strand, within CCNA_02190 at 2342.845 kb on + strand at 2342.851 kb on - strand at 2342.860 kb on - strand at 2342.974 kb on - strand at 2342.975 kb on - strand at 2342.981 kb on + strand at 2343.109 kb on + strand at 2343.187 kb on + strand, within CCNA_02191 at 2343.192 kb on + strand, within CCNA_02191 at 2343.200 kb on - strand, within CCNA_02191 at 2343.210 kb on - strand, within CCNA_02191 at 2343.210 kb on - strand, within CCNA_02191 at 2343.316 kb on - strand, within CCNA_02191 at 2343.316 kb on - strand, within CCNA_02191 at 2343.434 kb on - strand, within CCNA_02191 at 2343.436 kb on - strand, within CCNA_02191 at 2343.457 kb on + strand, within CCNA_02191 at 2343.462 kb on + strand, within CCNA_02191 at 2343.462 kb on + strand, within CCNA_02191 at 2343.470 kb on - strand, within CCNA_02191 at 2343.470 kb on - strand, within CCNA_02191 at 2343.486 kb on - strand at 2343.507 kb on + strand at 2343.515 kb on - strand at 2343.515 kb on - strand at 2343.613 kb on - strand, within CCNA_02192 at 2343.632 kb on + strand, within CCNA_02192 at 2343.632 kb on + strand, within CCNA_02192 at 2343.632 kb on + strand, within CCNA_02192 at 2343.640 kb on - strand, within CCNA_02192 at 2343.640 kb on - strand, within CCNA_02192 at 2343.654 kb on - strand, within CCNA_02192 at 2343.706 kb on + strand, within CCNA_02192 at 2343.708 kb on - strand, within CCNA_02192 at 2343.714 kb on - strand, within CCNA_02192 at 2343.715 kb on - strand, within CCNA_02192 at 2343.775 kb on + strand, within CCNA_02192 at 2343.778 kb on - strand, within CCNA_02192 at 2343.784 kb on + strand, within CCNA_02192 at 2343.805 kb on + strand, within CCNA_02192 at 2343.813 kb on - strand, within CCNA_02192 at 2343.815 kb on + strand, within CCNA_02192 at 2343.850 kb on + strand, within CCNA_02192 at 2343.854 kb on - strand, within CCNA_02192 at 2343.903 kb on + strand at 2343.911 kb on - strand at 2343.911 kb on - strand at 2343.911 kb on - strand at 2343.911 kb on - strand at 2343.946 kb on + strand, within CCNA_02193 at 2344.045 kb on + strand, within CCNA_02193 at 2344.066 kb on + strand, within CCNA_02193 at 2344.067 kb on + strand, within CCNA_02193 at 2344.110 kb on - strand, within CCNA_02193 at 2344.110 kb on - strand, within CCNA_02193 at 2344.120 kb on - strand, within CCNA_02193 at 2344.151 kb on + strand, within CCNA_02193 at 2344.214 kb on - strand, within CCNA_02193 at 2344.247 kb on + strand, within CCNA_02193 at 2344.255 kb on - strand, within CCNA_02193 at 2344.311 kb on - strand, within CCNA_02193 at 2344.351 kb on + strand, within CCNA_02193 at 2344.351 kb on + strand, within CCNA_02193 at 2344.353 kb on + strand, within CCNA_02193 at 2344.353 kb on + strand, within CCNA_02193 at 2344.359 kb on - strand at 2344.361 kb on - strand at 2344.369 kb on - strand at 2344.389 kb on + strand at 2344.408 kb on + strand at 2344.428 kb on + strand at 2344.449 kb on - strand at 2344.497 kb on + strand at 2344.497 kb on + strand at 2344.498 kb on + strand at 2344.517 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Chloramphenicol 0.0005 mg/ml remove 2,342,481 + CCNA_02190 0.74 +0.7 2,342,487 - CCNA_02190 0.74 -0.5 2,342,489 - CCNA_02190 0.74 -1.8 2,342,489 - CCNA_02190 0.74 -0.7 2,342,491 + CCNA_02190 0.74 +0.7 2,342,491 + CCNA_02190 0.74 -1.2 2,342,499 - CCNA_02190 0.74 -1.0 2,342,499 - CCNA_02190 0.74 -1.5 2,342,554 + CCNA_02190 0.77 -0.7 2,342,562 - CCNA_02190 0.77 -0.5 2,342,572 - CCNA_02190 0.78 -0.0 2,342,620 + CCNA_02190 0.80 +0.0 2,342,628 - CCNA_02190 0.80 -0.3 2,342,647 + CCNA_02190 0.81 -0.6 2,342,650 - CCNA_02190 0.81 +1.0 2,342,660 - CCNA_02190 0.82 -1.6 2,342,673 - CCNA_02190 0.83 -0.2 2,342,709 - CCNA_02190 0.84 +0.2 2,342,709 - CCNA_02190 0.84 -0.8 2,342,711 + CCNA_02190 0.84 +1.6 2,342,724 - CCNA_02190 0.85 +0.8 2,342,726 + CCNA_02190 0.85 -0.4 2,342,734 - CCNA_02190 0.85 +2.5 2,342,734 - CCNA_02190 0.85 -1.2 2,342,755 - CCNA_02190 0.86 -1.5 2,342,775 + CCNA_02190 0.87 -2.2 2,342,804 + CCNA_02190 0.89 +0.2 2,342,812 - CCNA_02190 0.89 -1.6 2,342,812 - CCNA_02190 0.89 +0.3 2,342,845 + +1.2 2,342,851 - +0.3 2,342,860 - +0.7 2,342,974 - +0.5 2,342,975 - -1.6 2,342,981 + +0.6 2,343,109 + +0.4 2,343,187 + CCNA_02191 0.16 +1.7 2,343,192 + CCNA_02191 0.17 -0.2 2,343,200 - CCNA_02191 0.19 -0.8 2,343,210 - CCNA_02191 0.21 -0.8 2,343,210 - CCNA_02191 0.21 -1.4 2,343,316 - CCNA_02191 0.49 -0.3 2,343,316 - CCNA_02191 0.49 -1.8 2,343,434 - CCNA_02191 0.79 -0.6 2,343,436 - CCNA_02191 0.80 -0.5 2,343,457 + CCNA_02191 0.85 -0.5 2,343,462 + CCNA_02191 0.87 -1.7 2,343,462 + CCNA_02191 0.87 -0.4 2,343,470 - CCNA_02191 0.89 -0.8 2,343,470 - CCNA_02191 0.89 -1.3 2,343,486 - -0.5 2,343,507 + +0.4 2,343,515 - -0.6 2,343,515 - +0.2 2,343,613 - CCNA_02192 0.30 -2.7 2,343,632 + CCNA_02192 0.34 -0.8 2,343,632 + CCNA_02192 0.34 +1.3 2,343,632 + CCNA_02192 0.34 +0.3 2,343,640 - CCNA_02192 0.36 -1.5 2,343,640 - CCNA_02192 0.36 +0.3 2,343,654 - CCNA_02192 0.39 -3.7 2,343,706 + CCNA_02192 0.51 -1.3 2,343,708 - CCNA_02192 0.51 -2.9 2,343,714 - CCNA_02192 0.53 +1.2 2,343,715 - CCNA_02192 0.53 +0.5 2,343,775 + CCNA_02192 0.67 -1.0 2,343,778 - CCNA_02192 0.67 -2.0 2,343,784 + CCNA_02192 0.69 -0.9 2,343,805 + CCNA_02192 0.73 -1.2 2,343,813 - CCNA_02192 0.75 -0.5 2,343,815 + CCNA_02192 0.76 -1.4 2,343,850 + CCNA_02192 0.84 -0.7 2,343,854 - CCNA_02192 0.85 -0.8 2,343,903 + -0.5 2,343,911 - -0.7 2,343,911 - -0.8 2,343,911 - -0.2 2,343,911 - +0.6 2,343,946 + CCNA_02193 0.13 -1.2 2,344,045 + CCNA_02193 0.31 +0.2 2,344,066 + CCNA_02193 0.35 -0.7 2,344,067 + CCNA_02193 0.35 +0.3 2,344,110 - CCNA_02193 0.43 -0.5 2,344,110 - CCNA_02193 0.43 -0.3 2,344,120 - CCNA_02193 0.45 -1.2 2,344,151 + CCNA_02193 0.51 -0.6 2,344,214 - CCNA_02193 0.63 -1.6 2,344,247 + CCNA_02193 0.69 -0.8 2,344,255 - CCNA_02193 0.70 -0.6 2,344,311 - CCNA_02193 0.81 -0.8 2,344,351 + CCNA_02193 0.88 -1.6 2,344,351 + CCNA_02193 0.88 +0.5 2,344,353 + CCNA_02193 0.89 -0.5 2,344,353 + CCNA_02193 0.89 -0.5 2,344,359 - +0.2 2,344,361 - -2.2 2,344,369 - -0.9 2,344,389 + -1.4 2,344,408 + +1.3 2,344,428 + -0.3 2,344,449 - -2.2 2,344,497 + +2.0 2,344,497 + -1.9 2,344,498 + -0.4 2,344,517 + +0.1
Or see this region's nucleotide sequence