Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00301

Experiment: PYE with Chloramphenicol 0.0005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00299 and CCNA_00300 are separated by 16 nucleotidesCCNA_00300 and CCNA_00301 are separated by 229 nucleotidesCCNA_00301 and CCNA_00302 are separated by 4 nucleotides CCNA_00299: CCNA_00299 - phosphoribosylamine--glycine ligase, at 312,678 to 313,961 _00299 CCNA_00300: CCNA_00300 - glyoxalase superfamily protein, at 313,978 to 314,385 _00300 CCNA_00301: CCNA_00301 - phosphotransferase family protein, at 314,615 to 315,697 _00301 CCNA_00302: CCNA_00302 - imidazolonepropionase-related amidohydrolase, at 315,702 to 316,991 _00302 Position (kb) 314 315 316Strain fitness (log2 ratio) -2 -1 0 1 2at 313.631 kb on + strand, within CCNA_00299at 313.680 kb on + strand, within CCNA_00299at 313.694 kb on + strand, within CCNA_00299at 313.800 kb on - strand, within CCNA_00299at 313.857 kb on - strandat 314.001 kb on + strandat 314.025 kb on + strand, within CCNA_00300at 314.029 kb on - strand, within CCNA_00300at 314.033 kb on - strand, within CCNA_00300at 314.033 kb on - strand, within CCNA_00300at 314.040 kb on - strand, within CCNA_00300at 314.064 kb on - strand, within CCNA_00300at 314.127 kb on + strand, within CCNA_00300at 314.135 kb on - strand, within CCNA_00300at 314.153 kb on + strand, within CCNA_00300at 314.153 kb on + strand, within CCNA_00300at 314.153 kb on + strand, within CCNA_00300at 314.153 kb on + strand, within CCNA_00300at 314.153 kb on + strand, within CCNA_00300at 314.155 kb on - strand, within CCNA_00300at 314.161 kb on - strand, within CCNA_00300at 314.161 kb on - strand, within CCNA_00300at 314.166 kb on - strand, within CCNA_00300at 314.166 kb on - strand, within CCNA_00300at 314.166 kb on - strand, within CCNA_00300at 314.166 kb on - strand, within CCNA_00300at 314.166 kb on - strand, within CCNA_00300at 314.186 kb on - strand, within CCNA_00300at 314.204 kb on - strand, within CCNA_00300at 314.205 kb on - strand, within CCNA_00300at 314.210 kb on - strand, within CCNA_00300at 314.238 kb on + strand, within CCNA_00300at 314.238 kb on + strand, within CCNA_00300at 314.246 kb on - strand, within CCNA_00300at 314.246 kb on - strand, within CCNA_00300at 314.247 kb on - strand, within CCNA_00300at 314.256 kb on - strand, within CCNA_00300at 314.332 kb on - strand, within CCNA_00300at 314.339 kb on + strand, within CCNA_00300at 314.380 kb on - strandat 314.392 kb on + strandat 314.392 kb on - strandat 314.399 kb on - strandat 314.400 kb on - strandat 314.445 kb on + strandat 314.471 kb on - strandat 314.503 kb on - strandat 314.551 kb on + strandat 314.580 kb on - strandat 314.635 kb on - strandat 314.739 kb on - strand, within CCNA_00301at 314.739 kb on - strand, within CCNA_00301at 314.745 kb on + strand, within CCNA_00301at 314.769 kb on - strand, within CCNA_00301at 314.802 kb on - strand, within CCNA_00301at 314.821 kb on - strand, within CCNA_00301at 314.864 kb on + strand, within CCNA_00301at 314.885 kb on - strand, within CCNA_00301at 314.889 kb on + strand, within CCNA_00301at 314.907 kb on - strand, within CCNA_00301at 314.907 kb on - strand, within CCNA_00301at 314.934 kb on - strand, within CCNA_00301at 314.936 kb on - strand, within CCNA_00301at 314.938 kb on + strand, within CCNA_00301at 314.938 kb on + strand, within CCNA_00301at 314.938 kb on + strand, within CCNA_00301at 314.946 kb on - strand, within CCNA_00301at 314.946 kb on - strand, within CCNA_00301at 314.946 kb on - strand, within CCNA_00301at 314.975 kb on + strand, within CCNA_00301at 314.975 kb on - strand, within CCNA_00301at 315.002 kb on + strand, within CCNA_00301at 315.032 kb on - strand, within CCNA_00301at 315.059 kb on + strand, within CCNA_00301at 315.067 kb on - strand, within CCNA_00301at 315.080 kb on - strand, within CCNA_00301at 315.088 kb on - strand, within CCNA_00301at 315.088 kb on - strand, within CCNA_00301at 315.093 kb on - strand, within CCNA_00301at 315.165 kb on + strand, within CCNA_00301at 315.175 kb on + strand, within CCNA_00301at 315.175 kb on + strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.183 kb on - strand, within CCNA_00301at 315.206 kb on - strand, within CCNA_00301at 315.259 kb on + strand, within CCNA_00301at 315.259 kb on + strand, within CCNA_00301at 315.294 kb on + strand, within CCNA_00301at 315.316 kb on + strand, within CCNA_00301at 315.332 kb on + strand, within CCNA_00301at 315.332 kb on + strand, within CCNA_00301at 315.346 kb on + strand, within CCNA_00301at 315.353 kb on + strand, within CCNA_00301at 315.353 kb on + strand, within CCNA_00301at 315.361 kb on - strand, within CCNA_00301at 315.399 kb on + strandat 315.408 kb on + strand, within CCNA_00301at 315.416 kb on - strand, within CCNA_00301at 315.416 kb on - strand, within CCNA_00301at 315.418 kb on + strand, within CCNA_00301at 315.430 kb on + strand, within CCNA_00301at 315.520 kb on + strand, within CCNA_00301at 315.543 kb on + strand, within CCNA_00301at 315.550 kb on + strand, within CCNA_00301at 315.577 kb on + strand, within CCNA_00301at 315.636 kb on - strandat 315.650 kb on + strandat 315.655 kb on + strandat 315.655 kb on + strandat 315.658 kb on - strandat 315.673 kb on - strandat 315.673 kb on - strandat 315.684 kb on + strandat 315.692 kb on - strandat 315.738 kb on + strandat 315.746 kb on - strandat 315.746 kb on - strandat 315.772 kb on - strandat 315.784 kb on - strandat 315.843 kb on + strand, within CCNA_00302at 315.843 kb on + strand, within CCNA_00302at 315.892 kb on - strand, within CCNA_00302at 315.931 kb on + strand, within CCNA_00302at 315.931 kb on + strand, within CCNA_00302at 315.931 kb on + strand, within CCNA_00302at 315.983 kb on - strand, within CCNA_00302at 316.028 kb on - strand, within CCNA_00302at 316.028 kb on - strand, within CCNA_00302at 316.028 kb on - strand, within CCNA_00302at 316.040 kb on - strand, within CCNA_00302at 316.068 kb on - strand, within CCNA_00302at 316.133 kb on - strand, within CCNA_00302at 316.167 kb on - strand, within CCNA_00302at 316.275 kb on - strand, within CCNA_00302at 316.336 kb on - strand, within CCNA_00302at 316.336 kb on - strand, within CCNA_00302at 316.353 kb on + strand, within CCNA_00302at 316.358 kb on + strand, within CCNA_00302at 316.358 kb on + strand, within CCNA_00302at 316.366 kb on - strand, within CCNA_00302at 316.366 kb on - strand, within CCNA_00302at 316.438 kb on + strand, within CCNA_00302at 316.438 kb on + strand, within CCNA_00302at 316.440 kb on - strand, within CCNA_00302at 316.447 kb on - strand, within CCNA_00302at 316.532 kb on - strand, within CCNA_00302at 316.553 kb on - strand, within CCNA_00302at 316.559 kb on + strand, within CCNA_00302at 316.567 kb on - strand, within CCNA_00302at 316.567 kb on - strand, within CCNA_00302at 316.567 kb on - strand, within CCNA_00302at 316.659 kb on - strand, within CCNA_00302at 316.680 kb on - strand, within CCNA_00302at 316.695 kb on - strand, within CCNA_00302

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Chloramphenicol 0.0005 mg/ml
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313,631 + CCNA_00299 0.74 +1.4
313,680 + CCNA_00299 0.78 -0.1
313,694 + CCNA_00299 0.79 +1.9
313,800 - CCNA_00299 0.87 +0.9
313,857 - +2.2
314,001 + +0.8
314,025 + CCNA_00300 0.12 -0.3
314,029 - CCNA_00300 0.12 -1.0
314,033 - CCNA_00300 0.13 +0.0
314,033 - CCNA_00300 0.13 -1.6
314,040 - CCNA_00300 0.15 +0.0
314,064 - CCNA_00300 0.21 -1.2
314,127 + CCNA_00300 0.37 -0.0
314,135 - CCNA_00300 0.38 -0.4
314,153 + CCNA_00300 0.43 -0.5
314,153 + CCNA_00300 0.43 +0.3
314,153 + CCNA_00300 0.43 -0.1
314,153 + CCNA_00300 0.43 -0.7
314,153 + CCNA_00300 0.43 -2.3
314,155 - CCNA_00300 0.43 -1.9
314,161 - CCNA_00300 0.45 +0.9
314,161 - CCNA_00300 0.45 -1.7
314,166 - CCNA_00300 0.46 -1.7
314,166 - CCNA_00300 0.46 -0.2
314,166 - CCNA_00300 0.46 -2.4
314,166 - CCNA_00300 0.46 +1.2
314,166 - CCNA_00300 0.46 +0.4
314,186 - CCNA_00300 0.51 +0.5
314,204 - CCNA_00300 0.55 -0.6
314,205 - CCNA_00300 0.56 -0.6
314,210 - CCNA_00300 0.57 -1.1
314,238 + CCNA_00300 0.64 +1.2
314,238 + CCNA_00300 0.64 +1.4
314,246 - CCNA_00300 0.66 -0.8
314,246 - CCNA_00300 0.66 -1.1
314,247 - CCNA_00300 0.66 -0.4
314,256 - CCNA_00300 0.68 -2.0
314,332 - CCNA_00300 0.87 -1.4
314,339 + CCNA_00300 0.88 -1.1
314,380 - -0.0
314,392 + +0.9
314,392 - +0.1
314,399 - +0.7
314,400 - -0.1
314,445 + +0.2
314,471 - +0.3
314,503 - +0.9
314,551 + -0.3
314,580 - -0.0
314,635 - -1.3
314,739 - CCNA_00301 0.11 -0.7
314,739 - CCNA_00301 0.11 -0.8
314,745 + CCNA_00301 0.12 -1.0
314,769 - CCNA_00301 0.14 -0.0
314,802 - CCNA_00301 0.17 +0.1
314,821 - CCNA_00301 0.19 -0.4
314,864 + CCNA_00301 0.23 -0.3
314,885 - CCNA_00301 0.25 +0.5
314,889 + CCNA_00301 0.25 -0.1
314,907 - CCNA_00301 0.27 +0.5
314,907 - CCNA_00301 0.27 +0.4
314,934 - CCNA_00301 0.29 +0.1
314,936 - CCNA_00301 0.30 +0.2
314,938 + CCNA_00301 0.30 -1.5
314,938 + CCNA_00301 0.30 -1.3
314,938 + CCNA_00301 0.30 +0.8
314,946 - CCNA_00301 0.31 -1.6
314,946 - CCNA_00301 0.31 +0.9
314,946 - CCNA_00301 0.31 -1.8
314,975 + CCNA_00301 0.33 -2.7
314,975 - CCNA_00301 0.33 -1.8
315,002 + CCNA_00301 0.36 -1.2
315,032 - CCNA_00301 0.39 -0.2
315,059 + CCNA_00301 0.41 -1.0
315,067 - CCNA_00301 0.42 -1.3
315,080 - CCNA_00301 0.43 -0.6
315,088 - CCNA_00301 0.44 -0.3
315,088 - CCNA_00301 0.44 -0.7
315,093 - CCNA_00301 0.44 -0.9
315,165 + CCNA_00301 0.51 -1.3
315,175 + CCNA_00301 0.52 -0.5
315,175 + CCNA_00301 0.52 -1.1
315,183 - CCNA_00301 0.52 -1.0
315,183 - CCNA_00301 0.52 +1.6
315,183 - CCNA_00301 0.52 -0.6
315,183 - CCNA_00301 0.52 -0.7
315,183 - CCNA_00301 0.52 -0.8
315,183 - CCNA_00301 0.52 -1.0
315,183 - CCNA_00301 0.52 -0.7
315,183 - CCNA_00301 0.52 +1.7
315,183 - CCNA_00301 0.52 -0.1
315,183 - CCNA_00301 0.52 -1.5
315,206 - CCNA_00301 0.55 -1.4
315,259 + CCNA_00301 0.59 -0.0
315,259 + CCNA_00301 0.59 -1.4
315,294 + CCNA_00301 0.63 +0.4
315,316 + CCNA_00301 0.65 +0.5
315,332 + CCNA_00301 0.66 -0.8
315,332 + CCNA_00301 0.66 -0.9
315,346 + CCNA_00301 0.67 -0.5
315,353 + CCNA_00301 0.68 -0.6
315,353 + CCNA_00301 0.68 -2.4
315,361 - CCNA_00301 0.69 +0.9
315,399 + +1.7
315,408 + CCNA_00301 0.73 -0.6
315,416 - CCNA_00301 0.74 -0.2
315,416 - CCNA_00301 0.74 -0.8
315,418 + CCNA_00301 0.74 -1.1
315,430 + CCNA_00301 0.75 +0.8
315,520 + CCNA_00301 0.84 +0.4
315,543 + CCNA_00301 0.86 -1.0
315,550 + CCNA_00301 0.86 -0.3
315,577 + CCNA_00301 0.89 -0.8
315,636 - -1.1
315,650 + -0.6
315,655 + +0.1
315,655 + -1.0
315,658 - +0.1
315,673 - -0.2
315,673 - -1.7
315,684 + +0.7
315,692 - -1.3
315,738 + -1.6
315,746 - -1.6
315,746 - -0.4
315,772 - -0.4
315,784 - -0.3
315,843 + CCNA_00302 0.11 +0.7
315,843 + CCNA_00302 0.11 -0.4
315,892 - CCNA_00302 0.15 -1.5
315,931 + CCNA_00302 0.18 +0.5
315,931 + CCNA_00302 0.18 +1.2
315,931 + CCNA_00302 0.18 -1.1
315,983 - CCNA_00302 0.22 +0.5
316,028 - CCNA_00302 0.25 -0.2
316,028 - CCNA_00302 0.25 +0.9
316,028 - CCNA_00302 0.25 -1.5
316,040 - CCNA_00302 0.26 -0.4
316,068 - CCNA_00302 0.28 +0.5
316,133 - CCNA_00302 0.33 -0.8
316,167 - CCNA_00302 0.36 +0.1
316,275 - CCNA_00302 0.44 -0.6
316,336 - CCNA_00302 0.49 -2.7
316,336 - CCNA_00302 0.49 -1.9
316,353 + CCNA_00302 0.50 -2.1
316,358 + CCNA_00302 0.51 +0.4
316,358 + CCNA_00302 0.51 +0.4
316,366 - CCNA_00302 0.51 +0.1
316,366 - CCNA_00302 0.51 -0.8
316,438 + CCNA_00302 0.57 +0.2
316,438 + CCNA_00302 0.57 -1.4
316,440 - CCNA_00302 0.57 -1.4
316,447 - CCNA_00302 0.58 -2.1
316,532 - CCNA_00302 0.64 +0.2
316,553 - CCNA_00302 0.66 +1.1
316,559 + CCNA_00302 0.66 +1.7
316,567 - CCNA_00302 0.67 -1.1
316,567 - CCNA_00302 0.67 -1.5
316,567 - CCNA_00302 0.67 -1.7
316,659 - CCNA_00302 0.74 -2.0
316,680 - CCNA_00302 0.76 -0.5
316,695 - CCNA_00302 0.77 -1.6

Or see this region's nucleotide sequence