Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_737

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_735 and Pf1N1B4_736 overlap by 14 nucleotidesPf1N1B4_736 and Pf1N1B4_737 overlap by 4 nucleotidesPf1N1B4_737 and Pf1N1B4_738 overlap by 8 nucleotidesPf1N1B4_738 and Pf1N1B4_739 are separated by 25 nucleotides Pf1N1B4_735 - Electron transport complex protein RnfB, at 838,162 to 839,169 _735 Pf1N1B4_736 - Electron transport complex protein RnfD, at 839,156 to 840,148 _736 Pf1N1B4_737 - Electron transport complex protein RnfG, at 840,145 to 840,756 _737 Pf1N1B4_738 - Electron transport complex protein RnfE, at 840,749 to 841,339 _738 Pf1N1B4_739 - Endonuclease III (EC 4.2.99.18), at 841,365 to 842,003 _739 Position (kb) 840 841Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 839.155 kb on + strandat 839.156 kb on - strandat 839.156 kb on - strandat 839.208 kb on + strandat 839.253 kb on - strandat 839.322 kb on + strand, within Pf1N1B4_736at 839.322 kb on + strand, within Pf1N1B4_736at 839.411 kb on + strand, within Pf1N1B4_736at 839.474 kb on + strand, within Pf1N1B4_736at 839.474 kb on + strand, within Pf1N1B4_736at 839.474 kb on + strand, within Pf1N1B4_736at 839.474 kb on + strand, within Pf1N1B4_736at 839.875 kb on + strand, within Pf1N1B4_736at 839.994 kb on + strand, within Pf1N1B4_736at 840.026 kb on + strand, within Pf1N1B4_736at 840.026 kb on + strand, within Pf1N1B4_736at 840.026 kb on + strand, within Pf1N1B4_736at 840.027 kb on - strand, within Pf1N1B4_736at 840.208 kb on + strand, within Pf1N1B4_737at 840.208 kb on + strand, within Pf1N1B4_737at 840.208 kb on + strand, within Pf1N1B4_737at 840.208 kb on + strand, within Pf1N1B4_737at 840.209 kb on - strand, within Pf1N1B4_737at 840.301 kb on + strand, within Pf1N1B4_737at 840.302 kb on - strand, within Pf1N1B4_737at 840.302 kb on - strand, within Pf1N1B4_737at 840.377 kb on - strand, within Pf1N1B4_737at 840.377 kb on - strand, within Pf1N1B4_737at 840.485 kb on + strand, within Pf1N1B4_737at 840.485 kb on + strand, within Pf1N1B4_737at 840.485 kb on + strand, within Pf1N1B4_737at 840.485 kb on + strand, within Pf1N1B4_737at 840.486 kb on - strand, within Pf1N1B4_737at 840.555 kb on + strand, within Pf1N1B4_737at 840.555 kb on + strand, within Pf1N1B4_737at 840.556 kb on - strand, within Pf1N1B4_737at 840.556 kb on - strand, within Pf1N1B4_737at 840.706 kb on + strandat 840.706 kb on + strandat 840.707 kb on - strandat 840.707 kb on - strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.769 kb on - strandat 840.788 kb on - strandat 840.866 kb on + strand, within Pf1N1B4_738at 840.867 kb on - strand, within Pf1N1B4_738at 840.867 kb on - strand, within Pf1N1B4_738at 840.867 kb on - strand, within Pf1N1B4_738at 840.879 kb on + strand, within Pf1N1B4_738at 840.879 kb on + strand, within Pf1N1B4_738at 840.879 kb on + strandat 840.879 kb on + strandat 840.880 kb on - strand, within Pf1N1B4_738at 840.880 kb on - strand, within Pf1N1B4_738at 840.933 kb on + strand, within Pf1N1B4_738at 841.013 kb on + strand, within Pf1N1B4_738at 841.273 kb on + strand, within Pf1N1B4_738at 841.274 kb on - strand, within Pf1N1B4_738at 841.281 kb on + strandat 841.288 kb on - strandat 841.440 kb on + strand, within Pf1N1B4_739at 841.441 kb on - strand, within Pf1N1B4_739at 841.441 kb on - strand, within Pf1N1B4_739at 841.542 kb on - strand, within Pf1N1B4_739at 841.587 kb on + strand, within Pf1N1B4_739at 841.587 kb on + strand, within Pf1N1B4_739at 841.588 kb on - strand, within Pf1N1B4_739at 841.648 kb on + strand, within Pf1N1B4_739at 841.649 kb on - strand, within Pf1N1B4_739at 841.675 kb on + strand, within Pf1N1B4_739

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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839,155 + -0.2
839,156 - -0.7
839,156 - +0.9
839,208 + +0.2
839,253 - -1.7
839,322 + Pf1N1B4_736 0.17 -0.3
839,322 + Pf1N1B4_736 0.17 -0.5
839,411 + Pf1N1B4_736 0.26 +0.3
839,474 + Pf1N1B4_736 0.32 +0.0
839,474 + Pf1N1B4_736 0.32 -0.1
839,474 + Pf1N1B4_736 0.32 -0.7
839,474 + Pf1N1B4_736 0.32 +1.8
839,875 + Pf1N1B4_736 0.72 +1.1
839,994 + Pf1N1B4_736 0.84 -0.6
840,026 + Pf1N1B4_736 0.88 +0.8
840,026 + Pf1N1B4_736 0.88 +1.3
840,026 + Pf1N1B4_736 0.88 -1.5
840,027 - Pf1N1B4_736 0.88 -2.0
840,208 + Pf1N1B4_737 0.10 +0.3
840,208 + Pf1N1B4_737 0.10 -1.5
840,208 + Pf1N1B4_737 0.10 -0.5
840,208 + Pf1N1B4_737 0.10 -1.0
840,209 - Pf1N1B4_737 0.10 +0.7
840,301 + Pf1N1B4_737 0.25 +2.2
840,302 - Pf1N1B4_737 0.26 +0.2
840,302 - Pf1N1B4_737 0.26 -0.1
840,377 - Pf1N1B4_737 0.38 +0.7
840,377 - Pf1N1B4_737 0.38 -2.9
840,485 + Pf1N1B4_737 0.56 +0.9
840,485 + Pf1N1B4_737 0.56 +0.3
840,485 + Pf1N1B4_737 0.56 +0.0
840,485 + Pf1N1B4_737 0.56 -0.4
840,486 - Pf1N1B4_737 0.56 +1.3
840,555 + Pf1N1B4_737 0.67 +1.0
840,555 + Pf1N1B4_737 0.67 +1.1
840,556 - Pf1N1B4_737 0.67 +0.3
840,556 - Pf1N1B4_737 0.67 +0.3
840,706 + -0.2
840,706 + -1.4
840,707 - -0.8
840,707 - -1.5
840,754 + +2.7
840,754 + -0.4
840,754 + +0.9
840,754 + +0.3
840,754 + +0.9
840,754 + -0.2
840,755 - +0.0
840,755 - +0.0
840,755 - +0.8
840,755 - +0.3
840,755 - +0.9
840,755 - +0.1
840,755 - +0.7
840,755 - +0.3
840,769 - +0.3
840,788 - -2.2
840,866 + Pf1N1B4_738 0.20 -1.2
840,867 - Pf1N1B4_738 0.20 -0.2
840,867 - Pf1N1B4_738 0.20 -1.0
840,867 - Pf1N1B4_738 0.20 +0.7
840,879 + Pf1N1B4_738 0.22 +0.5
840,879 + Pf1N1B4_738 0.22 -0.2
840,879 + +0.3
840,879 + +1.0
840,880 - Pf1N1B4_738 0.22 +0.4
840,880 - Pf1N1B4_738 0.22 -1.2
840,933 + Pf1N1B4_738 0.31 -0.5
841,013 + Pf1N1B4_738 0.45 -0.7
841,273 + Pf1N1B4_738 0.89 +1.3
841,274 - Pf1N1B4_738 0.89 -0.2
841,281 + +0.5
841,288 - +0.1
841,440 + Pf1N1B4_739 0.12 -1.5
841,441 - Pf1N1B4_739 0.12 +0.5
841,441 - Pf1N1B4_739 0.12 +0.5
841,542 - Pf1N1B4_739 0.28 +2.1
841,587 + Pf1N1B4_739 0.35 -0.9
841,587 + Pf1N1B4_739 0.35 +1.9
841,588 - Pf1N1B4_739 0.35 +0.8
841,648 + Pf1N1B4_739 0.44 +0.3
841,649 - Pf1N1B4_739 0.44 -0.7
841,675 + Pf1N1B4_739 0.49 -0.9

Or see this region's nucleotide sequence