Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5689

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5686 and Pf1N1B4_5687 are separated by 132 nucleotidesPf1N1B4_5687 and Pf1N1B4_5688 are separated by 57 nucleotidesPf1N1B4_5688 and Pf1N1B4_5689 are separated by 11 nucleotidesPf1N1B4_5689 and Pf1N1B4_5690 are separated by 70 nucleotidesPf1N1B4_5690 and Pf1N1B4_6053 are separated by 79 nucleotides Pf1N1B4_5686 - Permease of the drug/metabolite transporter (DMT) superfamily, at 125,838 to 126,239 _5686 Pf1N1B4_5687 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 126,372 to 126,809 _5687 Pf1N1B4_5688 - hypothetical protein, at 126,867 to 127,196 _5688 Pf1N1B4_5689 - Lactoylglutathione lyase, at 127,208 to 127,600 _5689 Pf1N1B4_5690 - Putative TEGT family carrier/transport protein, at 127,671 to 128,387 _5690 Pf1N1B4_6053 - tRNA-Ser-TGA, at 128,467 to 128,551 _6053 Position (kb) 127 128Strain fitness (log2 ratio) -2 -1 0 1 2at 126.222 kb on + strandat 126.223 kb on - strandat 126.250 kb on + strandat 126.251 kb on - strandat 126.522 kb on + strand, within Pf1N1B4_5687at 126.523 kb on - strand, within Pf1N1B4_5687at 126.834 kb on - strandat 126.901 kb on + strand, within Pf1N1B4_5688at 127.005 kb on + strand, within Pf1N1B4_5688at 127.005 kb on + strand, within Pf1N1B4_5688at 127.005 kb on + strand, within Pf1N1B4_5688at 127.006 kb on - strandat 127.020 kb on + strand, within Pf1N1B4_5688at 127.068 kb on + strand, within Pf1N1B4_5688at 127.069 kb on - strand, within Pf1N1B4_5688at 127.074 kb on + strandat 127.074 kb on + strand, within Pf1N1B4_5688at 127.075 kb on - strand, within Pf1N1B4_5688at 127.075 kb on - strand, within Pf1N1B4_5688at 127.096 kb on + strand, within Pf1N1B4_5688at 127.097 kb on - strand, within Pf1N1B4_5688at 127.109 kb on - strand, within Pf1N1B4_5688at 127.155 kb on + strand, within Pf1N1B4_5688at 127.255 kb on - strandat 127.263 kb on - strand, within Pf1N1B4_5689at 127.460 kb on + strand, within Pf1N1B4_5689at 127.544 kb on + strand, within Pf1N1B4_5689at 127.545 kb on - strand, within Pf1N1B4_5689at 127.545 kb on - strand, within Pf1N1B4_5689at 127.545 kb on - strand, within Pf1N1B4_5689at 127.545 kb on - strandat 127.546 kb on + strand, within Pf1N1B4_5689at 127.546 kb on + strand, within Pf1N1B4_5689at 127.546 kb on + strand, within Pf1N1B4_5689at 127.630 kb on - strandat 127.727 kb on - strandat 127.727 kb on - strandat 127.735 kb on - strandat 127.735 kb on - strandat 127.739 kb on - strandat 127.763 kb on - strand, within Pf1N1B4_5690at 127.763 kb on - strand, within Pf1N1B4_5690at 127.966 kb on + strand, within Pf1N1B4_5690at 127.966 kb on + strand, within Pf1N1B4_5690at 127.967 kb on - strand, within Pf1N1B4_5690at 128.292 kb on + strand, within Pf1N1B4_5690

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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126,222 + -1.5
126,223 - +2.3
126,250 + -0.8
126,251 - -0.3
126,522 + Pf1N1B4_5687 0.34 +0.1
126,523 - Pf1N1B4_5687 0.34 +0.1
126,834 - -0.5
126,901 + Pf1N1B4_5688 0.10 -0.3
127,005 + Pf1N1B4_5688 0.42 -1.9
127,005 + Pf1N1B4_5688 0.42 -0.5
127,005 + Pf1N1B4_5688 0.42 +0.1
127,006 - +0.1
127,020 + Pf1N1B4_5688 0.46 -0.2
127,068 + Pf1N1B4_5688 0.61 -1.3
127,069 - Pf1N1B4_5688 0.61 -2.3
127,074 + -1.3
127,074 + Pf1N1B4_5688 0.63 -0.8
127,075 - Pf1N1B4_5688 0.63 +0.7
127,075 - Pf1N1B4_5688 0.63 +0.5
127,096 + Pf1N1B4_5688 0.69 -1.0
127,097 - Pf1N1B4_5688 0.70 -0.5
127,109 - Pf1N1B4_5688 0.73 -0.7
127,155 + Pf1N1B4_5688 0.87 +0.8
127,255 - +2.6
127,263 - Pf1N1B4_5689 0.14 -0.3
127,460 + Pf1N1B4_5689 0.64 +0.6
127,544 + Pf1N1B4_5689 0.85 -0.3
127,545 - Pf1N1B4_5689 0.86 -0.2
127,545 - Pf1N1B4_5689 0.86 -0.2
127,545 - Pf1N1B4_5689 0.86 +0.1
127,545 - +0.1
127,546 + Pf1N1B4_5689 0.86 +0.1
127,546 + Pf1N1B4_5689 0.86 -0.3
127,546 + Pf1N1B4_5689 0.86 -1.3
127,630 - -1.3
127,727 - +0.8
127,727 - +0.1
127,735 - +0.1
127,735 - -0.4
127,739 - -0.9
127,763 - Pf1N1B4_5690 0.13 -1.1
127,763 - Pf1N1B4_5690 0.13 +0.1
127,966 + Pf1N1B4_5690 0.41 +0.1
127,966 + Pf1N1B4_5690 0.41 -2.4
127,967 - Pf1N1B4_5690 0.41 -1.8
128,292 + Pf1N1B4_5690 0.87 -0.9

Or see this region's nucleotide sequence