Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5031 and Pf1N1B4_5032 are separated by 75 nucleotides Pf1N1B4_5032 and Pf1N1B4_5033 are separated by 69 nucleotides Pf1N1B4_5033 and Pf1N1B4_5034 are separated by 131 nucleotides Pf1N1B4_5034 and Pf1N1B4_5035 are separated by 306 nucleotides
Pf1N1B4_5031 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 5,576,918 to 5,577,997
_5031
Pf1N1B4_5032 - Hypothetical protein PA2244 (similar to DNA topoisomerase IB, but possibly involved in glycosyl-transfer), at 5,578,073 to 5,579,110
_5032
Pf1N1B4_5033 - hypothetical protein, at 5,579,180 to 5,579,437
_5033
Pf1N1B4_5034 - hypothetical protein, at 5,579,569 to 5,579,799
_5034
Pf1N1B4_5035 - hypothetical protein, at 5,580,106 to 5,580,390
_5035
Position (kb)
5578
5579
5580 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5577.375 kb on + strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.462 kb on + strand at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.463 kb on - strand, within Pf1N1B4_5031 at 5577.650 kb on + strand, within Pf1N1B4_5031 at 5577.868 kb on - strand, within Pf1N1B4_5031 at 5578.037 kb on + strand at 5578.119 kb on - strand at 5578.376 kb on + strand, within Pf1N1B4_5032 at 5578.377 kb on - strand, within Pf1N1B4_5032 at 5578.487 kb on - strand, within Pf1N1B4_5032 at 5578.487 kb on - strand, within Pf1N1B4_5032 at 5578.530 kb on - strand, within Pf1N1B4_5032 at 5578.772 kb on + strand, within Pf1N1B4_5032 at 5578.776 kb on + strand, within Pf1N1B4_5032 at 5578.777 kb on - strand at 5578.964 kb on + strand, within Pf1N1B4_5032 at 5578.965 kb on - strand at 5578.965 kb on - strand, within Pf1N1B4_5032 at 5579.035 kb on + strand at 5579.036 kb on - strand at 5579.036 kb on - strand at 5579.036 kb on - strand at 5579.130 kb on - strand at 5579.179 kb on + strand at 5579.179 kb on + strand at 5579.179 kb on + strand at 5579.180 kb on - strand at 5579.194 kb on + strand at 5579.194 kb on + strand at 5579.194 kb on + strand at 5579.194 kb on + strand at 5579.200 kb on + strand at 5579.200 kb on + strand at 5579.200 kb on + strand at 5579.200 kb on + strand at 5579.201 kb on - strand at 5579.201 kb on - strand at 5579.564 kb on - strand at 5579.772 kb on - strand, within Pf1N1B4_5034 at 5579.875 kb on + strand at 5579.876 kb on - strand at 5579.876 kb on - strand at 5580.044 kb on + strand at 5580.044 kb on + strand at 5580.044 kb on + strand at 5580.045 kb on - strand at 5580.045 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 5,577,375 + Pf1N1B4_5031 0.42 -2.0 5,577,376 - Pf1N1B4_5031 0.42 +1.6 5,577,376 - Pf1N1B4_5031 0.42 +0.4 5,577,376 - Pf1N1B4_5031 0.42 -0.7 5,577,462 + Pf1N1B4_5031 0.50 -0.6 5,577,462 + +0.4 5,577,462 + Pf1N1B4_5031 0.50 -0.9 5,577,462 + Pf1N1B4_5031 0.50 -0.1 5,577,463 - Pf1N1B4_5031 0.50 -1.0 5,577,650 + Pf1N1B4_5031 0.68 +1.6 5,577,868 - Pf1N1B4_5031 0.88 +0.0 5,578,037 + -0.6 5,578,119 - +1.0 5,578,376 + Pf1N1B4_5032 0.29 -0.3 5,578,377 - Pf1N1B4_5032 0.29 +1.0 5,578,487 - Pf1N1B4_5032 0.40 -0.7 5,578,487 - Pf1N1B4_5032 0.40 -0.4 5,578,530 - Pf1N1B4_5032 0.44 +0.6 5,578,772 + Pf1N1B4_5032 0.67 -2.1 5,578,776 + Pf1N1B4_5032 0.68 -1.0 5,578,777 - +1.0 5,578,964 + Pf1N1B4_5032 0.86 -1.8 5,578,965 - +0.4 5,578,965 - Pf1N1B4_5032 0.86 -0.1 5,579,035 + +0.4 5,579,036 - +2.3 5,579,036 - -1.0 5,579,036 - -0.6 5,579,130 - +0.3 5,579,179 + -1.6 5,579,179 + +0.0 5,579,179 + -0.4 5,579,180 - +0.0 5,579,194 + -2.1 5,579,194 + -0.6 5,579,194 + -0.0 5,579,194 + +0.0 5,579,200 + -1.3 5,579,200 + -0.4 5,579,200 + -0.6 5,579,200 + +1.0 5,579,201 - +1.0 5,579,201 - +0.7 5,579,564 - -1.0 5,579,772 - Pf1N1B4_5034 0.88 -0.6 5,579,875 + -0.1 5,579,876 - -1.1 5,579,876 - -1.1 5,580,044 + +0.0 5,580,044 + +1.4 5,580,044 + -0.1 5,580,045 - -0.3 5,580,045 - -0.8
Or see this region's nucleotide sequence