Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4690 and Pf1N1B4_4691 overlap by 1 nucleotides Pf1N1B4_4691 and Pf1N1B4_4692 are separated by 454 nucleotides Pf1N1B4_4692 and Pf1N1B4_4693 are separated by 49 nucleotides
Pf1N1B4_4690 - probable membrane protein YPO3302, at 5,182,766 to 5,183,200
_4690
Pf1N1B4_4691 - CopG protein, at 5,183,200 to 5,183,646
_4691
Pf1N1B4_4692 - hypothetical protein, at 5,184,101 to 5,184,631
_4692
Pf1N1B4_4693 - Oxidoreductase, at 5,184,681 to 5,185,676
_4693
Position (kb) 5184
5185 Strain fitness (log2 ratio) -2
-1
0
1 at 5183.188 kb on - strand at 5183.264 kb on + strand, within Pf1N1B4_4691 at 5183.362 kb on + strand, within Pf1N1B4_4691 at 5183.362 kb on + strand, within Pf1N1B4_4691 at 5183.363 kb on - strand, within Pf1N1B4_4691 at 5183.406 kb on + strand, within Pf1N1B4_4691 at 5183.483 kb on + strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.696 kb on - strand at 5183.728 kb on + strand at 5183.728 kb on + strand at 5183.837 kb on + strand at 5183.921 kb on + strand at 5183.961 kb on - strand at 5184.005 kb on + strand at 5184.005 kb on + strand at 5184.116 kb on - strand at 5184.455 kb on + strand at 5184.493 kb on + strand, within Pf1N1B4_4692 at 5184.493 kb on + strand, within Pf1N1B4_4692 at 5184.494 kb on - strand, within Pf1N1B4_4692 at 5184.494 kb on - strand, within Pf1N1B4_4692 at 5184.533 kb on + strand, within Pf1N1B4_4692 at 5184.548 kb on + strand, within Pf1N1B4_4692 at 5184.548 kb on + strand at 5184.548 kb on + strand, within Pf1N1B4_4692 at 5184.548 kb on + strand, within Pf1N1B4_4692 at 5184.548 kb on + strand at 5184.548 kb on + strand at 5184.552 kb on + strand, within Pf1N1B4_4692 at 5184.552 kb on + strand, within Pf1N1B4_4692 at 5184.572 kb on + strand, within Pf1N1B4_4692 at 5184.818 kb on - strand, within Pf1N1B4_4693 at 5184.818 kb on - strand, within Pf1N1B4_4693 at 5184.821 kb on + strand, within Pf1N1B4_4693 at 5185.365 kb on + strand, within Pf1N1B4_4693 at 5185.365 kb on + strand, within Pf1N1B4_4693 at 5185.365 kb on + strand, within Pf1N1B4_4693 at 5185.365 kb on + strand at 5185.365 kb on + strand at 5185.366 kb on - strand, within Pf1N1B4_4693 at 5185.366 kb on - strand, within Pf1N1B4_4693 at 5185.366 kb on - strand at 5185.366 kb on - strand, within Pf1N1B4_4693 at 5185.369 kb on + strand, within Pf1N1B4_4693 at 5185.370 kb on - strand, within Pf1N1B4_4693 at 5185.370 kb on - strand, within Pf1N1B4_4693 at 5185.496 kb on + strand, within Pf1N1B4_4693 at 5185.497 kb on - strand, within Pf1N1B4_4693 at 5185.497 kb on - strand, within Pf1N1B4_4693 at 5185.508 kb on - strand, within Pf1N1B4_4693 at 5185.508 kb on - strand, within Pf1N1B4_4693 at 5185.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 5,183,188 - +0.1 5,183,264 + Pf1N1B4_4691 0.14 +0.3 5,183,362 + Pf1N1B4_4691 0.36 -0.3 5,183,362 + Pf1N1B4_4691 0.36 -1.3 5,183,363 - Pf1N1B4_4691 0.36 -0.3 5,183,406 + Pf1N1B4_4691 0.46 +0.7 5,183,483 + Pf1N1B4_4691 0.63 +0.7 5,183,484 - Pf1N1B4_4691 0.64 +0.1 5,183,484 - Pf1N1B4_4691 0.64 +0.7 5,183,484 - Pf1N1B4_4691 0.64 +0.1 5,183,696 - +0.4 5,183,728 + -1.7 5,183,728 + +0.5 5,183,837 + +0.7 5,183,921 + -0.5 5,183,961 - +0.5 5,184,005 + +0.3 5,184,005 + -1.7 5,184,116 - -0.9 5,184,455 + +0.1 5,184,493 + Pf1N1B4_4692 0.74 +1.1 5,184,493 + Pf1N1B4_4692 0.74 +0.5 5,184,494 - Pf1N1B4_4692 0.74 -1.3 5,184,494 - Pf1N1B4_4692 0.74 -0.7 5,184,533 + Pf1N1B4_4692 0.81 +0.6 5,184,548 + Pf1N1B4_4692 0.84 +0.1 5,184,548 + -0.5 5,184,548 + Pf1N1B4_4692 0.84 -0.1 5,184,548 + Pf1N1B4_4692 0.84 -0.1 5,184,548 + +1.1 5,184,548 + -0.5 5,184,552 + Pf1N1B4_4692 0.85 -2.1 5,184,552 + Pf1N1B4_4692 0.85 +1.1 5,184,572 + Pf1N1B4_4692 0.89 +0.9 5,184,818 - Pf1N1B4_4693 0.14 +0.9 5,184,818 - Pf1N1B4_4693 0.14 -2.1 5,184,821 + Pf1N1B4_4693 0.14 -0.5 5,185,365 + Pf1N1B4_4693 0.69 -0.1 5,185,365 + Pf1N1B4_4693 0.69 +0.1 5,185,365 + Pf1N1B4_4693 0.69 -0.5 5,185,365 + -0.5 5,185,365 + +0.5 5,185,366 - Pf1N1B4_4693 0.69 +0.6 5,185,366 - Pf1N1B4_4693 0.69 +1.5 5,185,366 - -0.3 5,185,366 - Pf1N1B4_4693 0.69 -0.9 5,185,369 + Pf1N1B4_4693 0.69 +1.1 5,185,370 - Pf1N1B4_4693 0.69 -0.1 5,185,370 - Pf1N1B4_4693 0.69 +0.8 5,185,496 + Pf1N1B4_4693 0.82 +0.1 5,185,497 - Pf1N1B4_4693 0.82 +0.1 5,185,497 - Pf1N1B4_4693 0.82 +1.7 5,185,508 - Pf1N1B4_4693 0.83 -0.7 5,185,508 - Pf1N1B4_4693 0.83 -0.2 5,185,598 - -0.9
Or see this region's nucleotide sequence