Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4355 and Pf1N1B4_4356 are separated by 52 nucleotides Pf1N1B4_4356 and Pf1N1B4_4357 are separated by 421 nucleotides
Pf1N1B4_4355 - Gamma-glutamyl-aminobutyraldehyde dehydrogenase (EC 1.2.1.-), at 4,812,132 to 4,813,622
_4355
Pf1N1B4_4356 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 4,813,675 to 4,814,973
_4356
Pf1N1B4_4357 - Putrescine utilization regulator, at 4,815,395 to 4,815,943
_4357
Position (kb)
4813
4814
4815 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.426 kb on + strand, within Pf1N1B4_4355 at 4813.500 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.588 kb on - strand at 4813.588 kb on - strand at 4813.588 kb on - strand at 4813.620 kb on + strand at 4813.629 kb on + strand at 4813.630 kb on - strand at 4813.675 kb on - strand at 4813.675 kb on - strand at 4813.706 kb on + strand at 4813.706 kb on + strand at 4813.706 kb on + strand at 4813.707 kb on - strand at 4813.715 kb on - strand at 4813.717 kb on + strand at 4814.197 kb on + strand, within Pf1N1B4_4356 at 4814.518 kb on + strand, within Pf1N1B4_4356 at 4814.518 kb on + strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.539 kb on + strand, within Pf1N1B4_4356 at 4814.540 kb on - strand, within Pf1N1B4_4356 at 4814.764 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.942 kb on - strand at 4814.967 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.972 kb on - strand at 4814.972 kb on - strand at 4815.017 kb on - strand at 4815.231 kb on - strand at 4815.231 kb on - strand at 4815.287 kb on - strand at 4815.287 kb on - strand at 4815.370 kb on + strand at 4815.370 kb on + strand at 4815.371 kb on - strand at 4815.375 kb on - strand at 4815.421 kb on - strand at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.612 kb on - strand, within Pf1N1B4_4357 at 4815.826 kb on + strand, within Pf1N1B4_4357
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 4,813,194 + Pf1N1B4_4355 0.71 -1.8 4,813,194 + Pf1N1B4_4355 0.71 -0.5 4,813,194 + Pf1N1B4_4355 0.71 -0.9 4,813,426 + Pf1N1B4_4355 0.87 -1.1 4,813,500 + -0.8 4,813,587 + +0.0 4,813,587 + -2.3 4,813,587 + -1.5 4,813,587 + -0.4 4,813,587 + +1.5 4,813,587 + -0.7 4,813,587 + -2.1 4,813,588 - -1.3 4,813,588 - +1.6 4,813,588 - -0.5 4,813,620 + -0.8 4,813,629 + +0.5 4,813,630 - +0.6 4,813,675 - -0.4 4,813,675 - +1.3 4,813,706 + -1.0 4,813,706 + -1.3 4,813,706 + -2.0 4,813,707 - -0.5 4,813,715 - -0.3 4,813,717 + -0.3 4,814,197 + Pf1N1B4_4356 0.40 -0.4 4,814,518 + Pf1N1B4_4356 0.65 -2.0 4,814,518 + Pf1N1B4_4356 0.65 -1.0 4,814,519 - Pf1N1B4_4356 0.65 +1.5 4,814,519 - Pf1N1B4_4356 0.65 +0.2 4,814,519 - Pf1N1B4_4356 0.65 +0.0 4,814,539 + Pf1N1B4_4356 0.67 -0.3 4,814,540 - Pf1N1B4_4356 0.67 +0.3 4,814,764 + Pf1N1B4_4356 0.84 -0.8 4,814,767 + +0.0 4,814,767 + Pf1N1B4_4356 0.84 -1.5 4,814,767 + Pf1N1B4_4356 0.84 -1.6 4,814,767 + +0.0 4,814,767 + Pf1N1B4_4356 0.84 +0.6 4,814,767 + Pf1N1B4_4356 0.84 -1.0 4,814,767 + Pf1N1B4_4356 0.84 -2.1 4,814,768 - Pf1N1B4_4356 0.84 -0.3 4,814,768 - Pf1N1B4_4356 0.84 +0.0 4,814,768 - Pf1N1B4_4356 0.84 -1.3 4,814,942 - +0.0 4,814,967 + +0.0 4,814,971 + +0.4 4,814,971 + +1.0 4,814,971 + +1.3 4,814,971 + -1.4 4,814,972 - -0.3 4,814,972 - +1.0 4,815,017 - +0.7 4,815,231 - +2.3 4,815,231 - +0.8 4,815,287 - -0.4 4,815,287 - -1.1 4,815,370 + +3.4 4,815,370 + -1.2 4,815,371 - -0.5 4,815,375 - +0.4 4,815,421 - -0.0 4,815,611 + Pf1N1B4_4357 0.39 -2.0 4,815,611 + Pf1N1B4_4357 0.39 +0.9 4,815,611 + Pf1N1B4_4357 0.39 -0.3 4,815,611 + Pf1N1B4_4357 0.39 -0.7 4,815,612 - Pf1N1B4_4357 0.40 -0.2 4,815,826 + Pf1N1B4_4357 0.79 +0.5
Or see this region's nucleotide sequence