Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3447 and Pf1N1B4_3448 are separated by 112 nucleotides Pf1N1B4_3448 and Pf1N1B4_3449 are separated by 573 nucleotides
Pf1N1B4_3447 - Succinylglutamate desuccinylase (EC 3.5.1.96), at 3,842,468 to 3,843,478
_3447
Pf1N1B4_3448 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 3,843,591 to 3,844,100
_3448
Pf1N1B4_3449 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 3,844,674 to 3,845,678
_3449
Position (kb)
3843
3844
3845 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3842.909 kb on + strand, within Pf1N1B4_3447 at 3842.960 kb on + strand, within Pf1N1B4_3447 at 3843.046 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.071 kb on + strand at 3843.071 kb on + strand at 3843.071 kb on + strand, within Pf1N1B4_3447 at 3843.072 kb on - strand, within Pf1N1B4_3447 at 3843.072 kb on - strand at 3843.072 kb on - strand, within Pf1N1B4_3447 at 3843.072 kb on - strand, within Pf1N1B4_3447 at 3843.073 kb on + strand, within Pf1N1B4_3447 at 3843.073 kb on + strand, within Pf1N1B4_3447 at 3843.074 kb on - strand, within Pf1N1B4_3447 at 3843.074 kb on - strand, within Pf1N1B4_3447 at 3843.077 kb on + strand, within Pf1N1B4_3447 at 3843.077 kb on + strand, within Pf1N1B4_3447 at 3843.077 kb on + strand, within Pf1N1B4_3447 at 3843.077 kb on + strand at 3843.078 kb on - strand, within Pf1N1B4_3447 at 3843.078 kb on - strand, within Pf1N1B4_3447 at 3843.078 kb on - strand, within Pf1N1B4_3447 at 3843.078 kb on - strand at 3843.078 kb on - strand, within Pf1N1B4_3447 at 3843.277 kb on + strand, within Pf1N1B4_3447 at 3843.335 kb on + strand, within Pf1N1B4_3447 at 3843.335 kb on + strand at 3843.335 kb on + strand, within Pf1N1B4_3447 at 3843.336 kb on - strand, within Pf1N1B4_3447 at 3843.336 kb on - strand, within Pf1N1B4_3447 at 3843.336 kb on - strand, within Pf1N1B4_3447 at 3843.476 kb on + strand at 3843.680 kb on + strand at 3843.680 kb on + strand, within Pf1N1B4_3448 at 3843.681 kb on - strand, within Pf1N1B4_3448 at 3843.681 kb on - strand at 3843.832 kb on + strand, within Pf1N1B4_3448 at 3843.832 kb on + strand, within Pf1N1B4_3448 at 3843.833 kb on - strand, within Pf1N1B4_3448 at 3843.833 kb on - strand, within Pf1N1B4_3448 at 3843.833 kb on - strand, within Pf1N1B4_3448 at 3843.833 kb on - strand, within Pf1N1B4_3448 at 3844.154 kb on - strand at 3844.159 kb on + strand at 3844.194 kb on + strand at 3844.194 kb on + strand at 3844.196 kb on + strand at 3844.196 kb on + strand at 3844.196 kb on + strand at 3844.239 kb on + strand at 3844.240 kb on - strand at 3844.240 kb on - strand at 3844.240 kb on - strand at 3844.240 kb on - strand at 3844.327 kb on + strand at 3844.417 kb on + strand at 3844.418 kb on - strand at 3844.424 kb on + strand at 3844.424 kb on + strand at 3844.424 kb on + strand at 3844.424 kb on + strand at 3844.425 kb on - strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.508 kb on - strand at 3844.606 kb on + strand at 3844.627 kb on + strand at 3844.628 kb on - strand at 3844.767 kb on + strand at 3844.768 kb on - strand at 3844.768 kb on - strand at 3844.768 kb on - strand at 3844.768 kb on - strand at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 3,842,909 + Pf1N1B4_3447 0.44 -2.2 3,842,960 + Pf1N1B4_3447 0.49 -0.5 3,843,046 + Pf1N1B4_3447 0.57 +0.3 3,843,071 + Pf1N1B4_3447 0.60 -1.9 3,843,071 + Pf1N1B4_3447 0.60 -2.8 3,843,071 + Pf1N1B4_3447 0.60 +0.3 3,843,071 + Pf1N1B4_3447 0.60 -1.5 3,843,071 + -2.1 3,843,071 + -1.1 3,843,071 + Pf1N1B4_3447 0.60 -1.7 3,843,072 - Pf1N1B4_3447 0.60 -1.7 3,843,072 - +0.3 3,843,072 - Pf1N1B4_3447 0.60 -3.3 3,843,072 - Pf1N1B4_3447 0.60 -2.3 3,843,073 + Pf1N1B4_3447 0.60 -2.1 3,843,073 + Pf1N1B4_3447 0.60 +1.3 3,843,074 - Pf1N1B4_3447 0.60 -1.5 3,843,074 - Pf1N1B4_3447 0.60 -2.3 3,843,077 + Pf1N1B4_3447 0.60 -0.9 3,843,077 + Pf1N1B4_3447 0.60 -1.1 3,843,077 + Pf1N1B4_3447 0.60 -2.7 3,843,077 + +0.3 3,843,078 - Pf1N1B4_3447 0.60 -1.3 3,843,078 - Pf1N1B4_3447 0.60 +0.3 3,843,078 - Pf1N1B4_3447 0.60 -0.7 3,843,078 - +1.3 3,843,078 - Pf1N1B4_3447 0.60 -2.5 3,843,277 + Pf1N1B4_3447 0.80 -0.7 3,843,335 + Pf1N1B4_3447 0.86 -1.5 3,843,335 + -0.7 3,843,335 + Pf1N1B4_3447 0.86 -1.7 3,843,336 - Pf1N1B4_3447 0.86 -1.5 3,843,336 - Pf1N1B4_3447 0.86 +1.3 3,843,336 - Pf1N1B4_3447 0.86 -0.3 3,843,476 + -1.3 3,843,680 + +0.3 3,843,680 + Pf1N1B4_3448 0.17 -1.7 3,843,681 - Pf1N1B4_3448 0.18 -0.9 3,843,681 - -1.1 3,843,832 + Pf1N1B4_3448 0.47 -2.4 3,843,832 + Pf1N1B4_3448 0.47 +0.7 3,843,833 - Pf1N1B4_3448 0.47 -1.6 3,843,833 - Pf1N1B4_3448 0.47 +0.4 3,843,833 - Pf1N1B4_3448 0.47 +0.3 3,843,833 - Pf1N1B4_3448 0.47 -1.1 3,844,154 - +0.5 3,844,159 + +0.3 3,844,194 + -0.5 3,844,194 + -1.1 3,844,196 + +1.1 3,844,196 + +0.0 3,844,196 + +0.5 3,844,239 + +0.3 3,844,240 - -0.0 3,844,240 - -1.6 3,844,240 - +0.7 3,844,240 - +0.8 3,844,327 + +0.3 3,844,417 + -0.5 3,844,418 - +0.3 3,844,424 + +0.6 3,844,424 + -0.3 3,844,424 + -0.7 3,844,424 + -0.1 3,844,425 - -1.7 3,844,507 + -1.6 3,844,507 + +0.5 3,844,507 + -0.3 3,844,507 + -0.2 3,844,507 + +1.6 3,844,507 + -0.8 3,844,507 + +0.3 3,844,508 - +0.0 3,844,606 + +1.0 3,844,627 + -0.1 3,844,628 - -1.9 3,844,767 + +0.7 3,844,768 - -0.7 3,844,768 - +1.3 3,844,768 - +0.5 3,844,768 - +0.9 3,844,905 + Pf1N1B4_3449 0.23 +0.3 3,844,905 + Pf1N1B4_3449 0.23 -0.2 3,844,905 + Pf1N1B4_3449 0.23 +0.5 3,844,905 + Pf1N1B4_3449 0.23 -0.4 3,844,905 + Pf1N1B4_3449 0.23 +1.3 3,844,906 - Pf1N1B4_3449 0.23 +0.1 3,844,906 - Pf1N1B4_3449 0.23 +0.5 3,844,906 - Pf1N1B4_3449 0.23 -0.5 3,844,906 - Pf1N1B4_3449 0.23 +0.1 3,844,906 - Pf1N1B4_3449 0.23 -0.7 3,844,906 - Pf1N1B4_3449 0.23 -2.4 3,844,906 - Pf1N1B4_3449 0.23 -0.1 3,844,932 + Pf1N1B4_3449 0.26 +0.1 3,844,932 + Pf1N1B4_3449 0.26 +0.5 3,844,932 + +0.8 3,844,932 + Pf1N1B4_3449 0.26 +1.3 3,844,932 + Pf1N1B4_3449 0.26 +0.9 3,844,932 + Pf1N1B4_3449 0.26 -1.7 3,844,932 + Pf1N1B4_3449 0.26 -1.0 3,844,933 - Pf1N1B4_3449 0.26 +2.0 3,844,933 - Pf1N1B4_3449 0.26 +0.8 3,844,933 - -1.3 3,844,933 - Pf1N1B4_3449 0.26 -0.9 3,844,933 - Pf1N1B4_3449 0.26 +0.5 3,844,933 - Pf1N1B4_3449 0.26 +0.4 3,844,933 - Pf1N1B4_3449 0.26 +0.7 3,844,933 - Pf1N1B4_3449 0.26 +1.7 3,844,933 - Pf1N1B4_3449 0.26 +1.3 3,844,933 - Pf1N1B4_3449 0.26 -1.3 3,844,933 - Pf1N1B4_3449 0.26 +0.1 3,844,933 - Pf1N1B4_3449 0.26 +0.7 3,844,934 + Pf1N1B4_3449 0.26 -1.3 3,844,934 + Pf1N1B4_3449 0.26 -0.5 3,844,934 + Pf1N1B4_3449 0.26 +1.7 3,844,934 + Pf1N1B4_3449 0.26 +0.7 3,844,934 + Pf1N1B4_3449 0.26 -0.9 3,844,934 + Pf1N1B4_3449 0.26 +2.5 3,844,934 + Pf1N1B4_3449 0.26 -1.1 3,844,935 - Pf1N1B4_3449 0.26 -1.9 3,844,935 - Pf1N1B4_3449 0.26 -2.1 3,844,935 - Pf1N1B4_3449 0.26 +0.1 3,844,935 - Pf1N1B4_3449 0.26 +0.7 3,844,935 - Pf1N1B4_3449 0.26 -0.7 3,844,935 - Pf1N1B4_3449 0.26 -1.6 3,844,935 - Pf1N1B4_3449 0.26 -1.3 3,844,939 - -1.1 3,844,939 - Pf1N1B4_3449 0.26 -1.1 3,844,939 - Pf1N1B4_3449 0.26 +1.4 3,844,939 - Pf1N1B4_3449 0.26 -0.4 3,844,939 - Pf1N1B4_3449 0.26 +1.3 3,844,939 - Pf1N1B4_3449 0.26 -0.5 3,844,939 - Pf1N1B4_3449 0.26 +0.8 3,844,939 - Pf1N1B4_3449 0.26 -0.5
Or see this region's nucleotide sequence