Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2078 and Pf1N1B4_2079 are separated by 92 nucleotides Pf1N1B4_2079 and Pf1N1B4_2080 are separated by 380 nucleotides
Pf1N1B4_2078 - lipase family protein, at 2,375,755 to 2,377,995
_2078
Pf1N1B4_2079 - lipoprotein, at 2,378,088 to 2,378,495
_2079
Pf1N1B4_2080 - hypothetical protein, at 2,378,876 to 2,379,724
_2080
Position (kb)
2378
2379 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2377.142 kb on + strand, within Pf1N1B4_2078 at 2377.142 kb on + strand, within Pf1N1B4_2078 at 2377.143 kb on - strand, within Pf1N1B4_2078 at 2377.154 kb on + strand, within Pf1N1B4_2078 at 2377.154 kb on + strand, within Pf1N1B4_2078 at 2377.154 kb on + strand at 2377.155 kb on - strand, within Pf1N1B4_2078 at 2377.155 kb on - strand, within Pf1N1B4_2078 at 2377.155 kb on - strand, within Pf1N1B4_2078 at 2377.155 kb on - strand, within Pf1N1B4_2078 at 2377.304 kb on + strand, within Pf1N1B4_2078 at 2377.342 kb on + strand, within Pf1N1B4_2078 at 2377.343 kb on - strand, within Pf1N1B4_2078 at 2377.429 kb on + strand, within Pf1N1B4_2078 at 2377.536 kb on + strand at 2377.537 kb on - strand, within Pf1N1B4_2078 at 2377.537 kb on - strand, within Pf1N1B4_2078 at 2377.588 kb on + strand, within Pf1N1B4_2078 at 2377.588 kb on + strand, within Pf1N1B4_2078 at 2377.588 kb on + strand, within Pf1N1B4_2078 at 2377.676 kb on + strand, within Pf1N1B4_2078 at 2377.676 kb on + strand, within Pf1N1B4_2078 at 2377.676 kb on + strand, within Pf1N1B4_2078 at 2377.676 kb on + strand, within Pf1N1B4_2078 at 2377.676 kb on + strand, within Pf1N1B4_2078 at 2377.677 kb on - strand, within Pf1N1B4_2078 at 2377.677 kb on - strand, within Pf1N1B4_2078 at 2377.677 kb on - strand, within Pf1N1B4_2078 at 2377.677 kb on - strand, within Pf1N1B4_2078 at 2377.762 kb on + strand, within Pf1N1B4_2078 at 2377.762 kb on + strand, within Pf1N1B4_2078 at 2377.762 kb on + strand, within Pf1N1B4_2078 at 2377.762 kb on + strand, within Pf1N1B4_2078 at 2377.768 kb on + strand, within Pf1N1B4_2078 at 2377.768 kb on + strand, within Pf1N1B4_2078 at 2377.769 kb on - strand, within Pf1N1B4_2078 at 2377.769 kb on - strand, within Pf1N1B4_2078 at 2377.791 kb on - strand at 2377.791 kb on - strand at 2377.818 kb on - strand at 2377.886 kb on - strand at 2377.922 kb on + strand at 2377.967 kb on + strand at 2377.967 kb on + strand at 2377.967 kb on + strand at 2377.997 kb on - strand at 2378.053 kb on - strand at 2378.053 kb on - strand at 2378.068 kb on + strand at 2378.069 kb on - strand at 2378.069 kb on - strand at 2378.086 kb on - strand at 2378.258 kb on + strand, within Pf1N1B4_2079 at 2378.258 kb on + strand, within Pf1N1B4_2079 at 2378.259 kb on - strand, within Pf1N1B4_2079 at 2378.259 kb on - strand at 2378.259 kb on - strand, within Pf1N1B4_2079 at 2378.259 kb on - strand, within Pf1N1B4_2079 at 2378.259 kb on - strand, within Pf1N1B4_2079 at 2378.259 kb on - strand, within Pf1N1B4_2079 at 2378.259 kb on - strand, within Pf1N1B4_2079 at 2378.274 kb on + strand, within Pf1N1B4_2079 at 2378.275 kb on - strand at 2378.275 kb on - strand, within Pf1N1B4_2079 at 2378.322 kb on + strand, within Pf1N1B4_2079 at 2378.322 kb on + strand, within Pf1N1B4_2079 at 2378.323 kb on - strand, within Pf1N1B4_2079 at 2378.338 kb on + strand, within Pf1N1B4_2079 at 2378.338 kb on + strand, within Pf1N1B4_2079 at 2378.339 kb on - strand, within Pf1N1B4_2079 at 2378.396 kb on + strand, within Pf1N1B4_2079 at 2378.397 kb on - strand, within Pf1N1B4_2079 at 2378.397 kb on - strand, within Pf1N1B4_2079 at 2378.397 kb on - strand, within Pf1N1B4_2079 at 2378.407 kb on - strand, within Pf1N1B4_2079 at 2378.470 kb on - strand at 2378.470 kb on - strand at 2378.549 kb on + strand at 2378.549 kb on + strand at 2378.549 kb on + strand at 2378.550 kb on - strand at 2378.550 kb on - strand at 2378.550 kb on - strand at 2378.631 kb on - strand at 2378.631 kb on - strand at 2378.632 kb on + strand at 2378.633 kb on - strand at 2378.633 kb on - strand at 2378.660 kb on - strand at 2378.660 kb on - strand at 2378.668 kb on + strand at 2378.668 kb on + strand at 2378.669 kb on - strand at 2378.669 kb on - strand at 2378.669 kb on - strand at 2378.693 kb on + strand at 2378.694 kb on - strand at 2378.740 kb on + strand at 2378.740 kb on + strand at 2378.741 kb on - strand at 2378.741 kb on - strand at 2378.758 kb on + strand at 2378.758 kb on + strand at 2378.758 kb on + strand at 2378.759 kb on - strand at 2378.759 kb on - strand at 2378.808 kb on - strand at 2378.868 kb on + strand at 2378.916 kb on + strand at 2378.916 kb on + strand at 2378.916 kb on + strand at 2378.917 kb on - strand at 2378.917 kb on - strand at 2378.918 kb on + strand at 2378.918 kb on + strand at 2378.918 kb on + strand at 2378.918 kb on + strand at 2378.918 kb on + strand at 2378.918 kb on + strand at 2378.919 kb on - strand at 2378.919 kb on - strand at 2378.919 kb on - strand at 2378.919 kb on - strand at 2379.047 kb on + strand, within Pf1N1B4_2080 at 2379.048 kb on - strand, within Pf1N1B4_2080 at 2379.048 kb on - strand, within Pf1N1B4_2080 at 2379.174 kb on + strand, within Pf1N1B4_2080 at 2379.315 kb on + strand, within Pf1N1B4_2080 at 2379.354 kb on - strand, within Pf1N1B4_2080 at 2379.376 kb on - strand, within Pf1N1B4_2080 at 2379.405 kb on - strand, within Pf1N1B4_2080 at 2379.491 kb on - strand, within Pf1N1B4_2080
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 2,377,142 + Pf1N1B4_2078 0.62 -2.1 2,377,142 + Pf1N1B4_2078 0.62 -0.3 2,377,143 - Pf1N1B4_2078 0.62 -0.1 2,377,154 + Pf1N1B4_2078 0.62 +1.1 2,377,154 + Pf1N1B4_2078 0.62 -0.5 2,377,154 + -0.8 2,377,155 - Pf1N1B4_2078 0.62 +0.9 2,377,155 - Pf1N1B4_2078 0.62 -0.3 2,377,155 - Pf1N1B4_2078 0.62 -2.0 2,377,155 - Pf1N1B4_2078 0.62 -1.3 2,377,304 + Pf1N1B4_2078 0.69 -0.5 2,377,342 + Pf1N1B4_2078 0.71 -0.8 2,377,343 - Pf1N1B4_2078 0.71 -0.1 2,377,429 + Pf1N1B4_2078 0.75 +0.8 2,377,536 + -0.1 2,377,537 - Pf1N1B4_2078 0.80 +0.5 2,377,537 - Pf1N1B4_2078 0.80 -1.3 2,377,588 + Pf1N1B4_2078 0.82 -1.1 2,377,588 + Pf1N1B4_2078 0.82 +0.8 2,377,588 + Pf1N1B4_2078 0.82 -0.1 2,377,676 + Pf1N1B4_2078 0.86 -2.4 2,377,676 + Pf1N1B4_2078 0.86 +1.1 2,377,676 + Pf1N1B4_2078 0.86 -0.2 2,377,676 + Pf1N1B4_2078 0.86 -1.5 2,377,676 + Pf1N1B4_2078 0.86 +0.0 2,377,677 - Pf1N1B4_2078 0.86 +0.3 2,377,677 - Pf1N1B4_2078 0.86 +0.1 2,377,677 - Pf1N1B4_2078 0.86 -2.1 2,377,677 - Pf1N1B4_2078 0.86 -0.5 2,377,762 + Pf1N1B4_2078 0.90 +1.7 2,377,762 + Pf1N1B4_2078 0.90 +0.2 2,377,762 + Pf1N1B4_2078 0.90 +0.7 2,377,762 + Pf1N1B4_2078 0.90 +0.9 2,377,768 + Pf1N1B4_2078 0.90 +0.0 2,377,768 + Pf1N1B4_2078 0.90 +0.5 2,377,769 - Pf1N1B4_2078 0.90 +2.5 2,377,769 - Pf1N1B4_2078 0.90 -0.8 2,377,791 - +0.3 2,377,791 - -0.1 2,377,818 - -0.1 2,377,886 - -1.0 2,377,922 + -0.1 2,377,967 + +1.7 2,377,967 + +2.0 2,377,967 + +1.2 2,377,997 - -0.1 2,378,053 - +0.5 2,378,053 - -0.1 2,378,068 + -0.8 2,378,069 - -0.1 2,378,069 - -0.4 2,378,086 - -0.0 2,378,258 + Pf1N1B4_2079 0.42 -0.7 2,378,258 + Pf1N1B4_2079 0.42 -0.5 2,378,259 - Pf1N1B4_2079 0.42 -1.1 2,378,259 - +2.5 2,378,259 - Pf1N1B4_2079 0.42 -0.1 2,378,259 - Pf1N1B4_2079 0.42 +0.2 2,378,259 - Pf1N1B4_2079 0.42 +0.8 2,378,259 - Pf1N1B4_2079 0.42 +0.0 2,378,259 - Pf1N1B4_2079 0.42 -0.5 2,378,274 + Pf1N1B4_2079 0.46 -1.6 2,378,275 - -0.5 2,378,275 - Pf1N1B4_2079 0.46 -0.5 2,378,322 + Pf1N1B4_2079 0.57 -1.2 2,378,322 + Pf1N1B4_2079 0.57 +0.5 2,378,323 - Pf1N1B4_2079 0.58 -0.6 2,378,338 + Pf1N1B4_2079 0.61 -0.5 2,378,338 + Pf1N1B4_2079 0.61 -0.7 2,378,339 - Pf1N1B4_2079 0.62 -0.3 2,378,396 + Pf1N1B4_2079 0.75 -0.8 2,378,397 - Pf1N1B4_2079 0.76 -0.5 2,378,397 - Pf1N1B4_2079 0.76 -0.6 2,378,397 - Pf1N1B4_2079 0.76 +1.1 2,378,407 - Pf1N1B4_2079 0.78 -1.1 2,378,470 - +0.4 2,378,470 - -0.2 2,378,549 + +0.5 2,378,549 + +0.3 2,378,549 + -0.1 2,378,550 - -2.7 2,378,550 - -1.8 2,378,550 - -0.3 2,378,631 - -1.3 2,378,631 - +1.5 2,378,632 + +0.2 2,378,633 - -1.5 2,378,633 - -0.2 2,378,660 - -0.7 2,378,660 - -1.8 2,378,668 + +0.3 2,378,668 + +0.9 2,378,669 - -0.1 2,378,669 - -0.1 2,378,669 - -1.7 2,378,693 + -0.4 2,378,694 - -1.6 2,378,740 + +0.5 2,378,740 + -1.3 2,378,741 - +1.2 2,378,741 - -1.1 2,378,758 + -0.8 2,378,758 + +0.5 2,378,758 + -0.1 2,378,759 - +1.4 2,378,759 - -0.1 2,378,808 - -0.1 2,378,868 + -1.1 2,378,916 + +0.5 2,378,916 + +0.3 2,378,916 + -1.5 2,378,917 - -2.1 2,378,917 - -1.7 2,378,918 + +1.3 2,378,918 + +0.8 2,378,918 + +1.5 2,378,918 + +0.7 2,378,918 + -1.0 2,378,918 + +0.5 2,378,919 - -0.7 2,378,919 - +0.5 2,378,919 - +0.9 2,378,919 - -0.2 2,379,047 + Pf1N1B4_2080 0.20 -1.1 2,379,048 - Pf1N1B4_2080 0.20 -0.8 2,379,048 - Pf1N1B4_2080 0.20 -0.5 2,379,174 + Pf1N1B4_2080 0.35 -1.6 2,379,315 + Pf1N1B4_2080 0.52 -1.1 2,379,354 - Pf1N1B4_2080 0.56 +1.5 2,379,376 - Pf1N1B4_2080 0.59 +0.7 2,379,405 - Pf1N1B4_2080 0.62 -1.7 2,379,491 - Pf1N1B4_2080 0.72 -1.7
Or see this region's nucleotide sequence