Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06590 and MMP_RS06595 are separated by 43 nucleotides MMP_RS06595 and MMP_RS06600 are separated by 37 nucleotides MMP_RS06600 and MMP_RS06605 are separated by 112 nucleotides MMP_RS06605 and MMP_RS06610 overlap by 4 nucleotides MMP_RS06610 and MMP_RS06615 are separated by 122 nucleotides
MMP_RS06590: MMP_RS06590 - GNAT family N-acetyltransferase, at 1,264,147 to 1,264,653
_RS06590
MMP_RS06595: MMP_RS06595 - flagellar hook-basal body complex protein FliE, at 1,264,697 to 1,265,248
_RS06595
MMP_RS06600: MMP_RS06600 - class III signal peptide-containing protein, at 1,265,286 to 1,265,489
_RS06600
MMP_RS06605: MMP_RS06605 - DUF5814 domain-containing protein, at 1,265,602 to 1,268,037
_RS06605
MMP_RS06610: MMP_RS06610 - DUF2334 domain-containing protein, at 1,268,034 to 1,268,834
_RS06610
MMP_RS06615: MMP_RS06615 - tRNA-Gly, at 1,268,957 to 1,269,029
_RS06615
Position (kb)
1265
1266
1267
1268
1269 Strain fitness (log2 ratio)
-1
0
1 at 1264.692 kb on + strand at 1264.703 kb on - strand at 1264.703 kb on - strand at 1265.287 kb on - strand at 1265.310 kb on - strand, within MMP_RS06600 at 1265.311 kb on + strand, within MMP_RS06600 at 1265.510 kb on - strand at 1265.511 kb on + strand at 1265.521 kb on - strand at 1265.526 kb on + strand at 1265.526 kb on + strand at 1265.544 kb on - strand at 1265.991 kb on + strand, within MMP_RS06605 at 1266.009 kb on - strand, within MMP_RS06605 at 1266.024 kb on - strand, within MMP_RS06605 at 1266.025 kb on + strand, within MMP_RS06605 at 1266.051 kb on + strand, within MMP_RS06605 at 1266.374 kb on - strand, within MMP_RS06605 at 1266.566 kb on - strand, within MMP_RS06605 at 1266.662 kb on + strand, within MMP_RS06605 at 1266.684 kb on + strand, within MMP_RS06605 at 1266.750 kb on + strand, within MMP_RS06605 at 1266.786 kb on + strand, within MMP_RS06605 at 1266.936 kb on + strand, within MMP_RS06605 at 1267.037 kb on - strand, within MMP_RS06605 at 1267.375 kb on + strand, within MMP_RS06605 at 1267.375 kb on + strand, within MMP_RS06605 at 1267.617 kb on - strand, within MMP_RS06605 at 1267.618 kb on + strand, within MMP_RS06605 at 1267.722 kb on + strand, within MMP_RS06605 at 1267.795 kb on + strand at 1267.910 kb on + strand at 1268.415 kb on + strand, within MMP_RS06610 at 1268.498 kb on - strand, within MMP_RS06610 at 1268.568 kb on + strand, within MMP_RS06610 at 1268.661 kb on + strand, within MMP_RS06610 at 1268.879 kb on - strand at 1268.890 kb on + strand at 1268.896 kb on + strand at 1268.897 kb on - strand at 1268.897 kb on - strand at 1268.898 kb on + strand at 1268.899 kb on - strand at 1268.899 kb on - strand at 1268.900 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,264,692 + +0.2 1,264,703 - -0.3 1,264,703 - +0.3 1,265,287 - -0.5 1,265,310 - MMP_RS06600 0.12 -0.2 1,265,311 + MMP_RS06600 0.12 -0.7 1,265,510 - -0.4 1,265,511 + +0.5 1,265,521 - +0.4 1,265,526 + -0.3 1,265,526 + +0.3 1,265,544 - -0.6 1,265,991 + MMP_RS06605 0.16 +0.5 1,266,009 - MMP_RS06605 0.17 -0.3 1,266,024 - MMP_RS06605 0.17 +0.0 1,266,025 + MMP_RS06605 0.17 -0.6 1,266,051 + MMP_RS06605 0.18 -0.7 1,266,374 - MMP_RS06605 0.32 -0.1 1,266,566 - MMP_RS06605 0.40 -0.1 1,266,662 + MMP_RS06605 0.44 -0.4 1,266,684 + MMP_RS06605 0.44 +0.2 1,266,750 + MMP_RS06605 0.47 +0.1 1,266,786 + MMP_RS06605 0.49 -0.4 1,266,936 + MMP_RS06605 0.55 -0.3 1,267,037 - MMP_RS06605 0.59 -0.6 1,267,375 + MMP_RS06605 0.73 -0.3 1,267,375 + MMP_RS06605 0.73 -0.3 1,267,617 - MMP_RS06605 0.83 -0.7 1,267,618 + MMP_RS06605 0.83 -0.3 1,267,722 + MMP_RS06605 0.87 -0.0 1,267,795 + +0.1 1,267,910 + +0.5 1,268,415 + MMP_RS06610 0.48 +1.2 1,268,498 - MMP_RS06610 0.58 +0.4 1,268,568 + MMP_RS06610 0.67 -0.2 1,268,661 + MMP_RS06610 0.78 -0.3 1,268,879 - -0.1 1,268,890 + -0.6 1,268,896 + -0.1 1,268,897 - +0.5 1,268,897 - -0.3 1,268,898 + -0.3 1,268,899 - +0.4 1,268,899 - -0.0 1,268,900 + +0.6
Or see this region's nucleotide sequence