Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02420 and MMP_RS02425 are separated by 119 nucleotides MMP_RS02425 and MMP_RS02430 are separated by 168 nucleotides
MMP_RS02420: MMP_RS02420 - DEAD/DEAH box helicase, at 450,214 to 451,830
_RS02420
MMP_RS02425: MMP_RS02425 - hypothetical protein, at 451,950 to 452,144
_RS02425
MMP_RS02430: MMP_RS02430 - hypothetical protein, at 452,313 to 453,959
_RS02430
Position (kb) 451
452
453 Strain fitness (log2 ratio) -1
0
1 at 451.154 kb on - strand at 451.239 kb on - strand at 451.240 kb on + strand at 451.276 kb on - strand at 451.335 kb on - strand at 451.336 kb on + strand at 451.338 kb on + strand at 451.496 kb on - strand at 451.602 kb on + strand at 451.614 kb on + strand at 451.707 kb on + strand at 451.832 kb on - strand at 451.958 kb on - strand at 451.988 kb on - strand, within MMP_RS02425 at 452.045 kb on - strand, within MMP_RS02425 at 452.049 kb on + strand, within MMP_RS02425 at 452.066 kb on - strand, within MMP_RS02425 at 452.082 kb on - strand, within MMP_RS02425 at 452.090 kb on - strand, within MMP_RS02425 at 452.107 kb on + strand, within MMP_RS02425 at 452.139 kb on - strand at 452.161 kb on - strand at 452.162 kb on + strand at 452.162 kb on + strand at 452.172 kb on - strand at 452.184 kb on - strand at 452.184 kb on - strand at 452.268 kb on + strand at 452.281 kb on - strand at 452.282 kb on + strand at 452.322 kb on - strand at 452.322 kb on - strand at 452.372 kb on - strand at 452.401 kb on - strand at 452.469 kb on + strand at 452.469 kb on + strand at 452.469 kb on + strand at 452.477 kb on - strand at 452.478 kb on + strand, within MMP_RS02430 at 452.481 kb on - strand, within MMP_RS02430 at 452.515 kb on - strand, within MMP_RS02430 at 452.558 kb on - strand, within MMP_RS02430 at 452.560 kb on - strand, within MMP_RS02430 at 452.601 kb on + strand, within MMP_RS02430 at 452.760 kb on + strand, within MMP_RS02430 at 452.791 kb on - strand, within MMP_RS02430 at 452.792 kb on + strand, within MMP_RS02430 at 452.862 kb on - strand, within MMP_RS02430 at 453.079 kb on + strand, within MMP_RS02430
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 451,154 - -0.4 451,239 - -0.2 451,240 + +0.4 451,276 - -0.2 451,335 - -0.2 451,336 + +1.0 451,338 + +0.3 451,496 - -0.2 451,602 + +0.4 451,614 + -0.2 451,707 + -0.1 451,832 - +0.6 451,958 - +0.0 451,988 - MMP_RS02425 0.19 +0.1 452,045 - MMP_RS02425 0.49 -0.4 452,049 + MMP_RS02425 0.51 +0.4 452,066 - MMP_RS02425 0.59 -0.2 452,082 - MMP_RS02425 0.68 -0.4 452,090 - MMP_RS02425 0.72 -0.1 452,107 + MMP_RS02425 0.81 -0.4 452,139 - +0.2 452,161 - +0.2 452,162 + -0.2 452,162 + +0.6 452,172 - -0.2 452,184 - -0.5 452,184 - -0.9 452,268 + -0.2 452,281 - -0.2 452,282 + -0.7 452,322 - +0.0 452,322 - +0.5 452,372 - +0.1 452,401 - +0.4 452,469 + -0.5 452,469 + -0.5 452,469 + -0.4 452,477 - -0.2 452,478 + MMP_RS02430 0.10 +0.4 452,481 - MMP_RS02430 0.10 +0.0 452,515 - MMP_RS02430 0.12 +0.0 452,558 - MMP_RS02430 0.15 +0.2 452,560 - MMP_RS02430 0.15 -0.1 452,601 + MMP_RS02430 0.17 -0.0 452,760 + MMP_RS02430 0.27 +0.5 452,791 - MMP_RS02430 0.29 +0.3 452,792 + MMP_RS02430 0.29 +0.4 452,862 - MMP_RS02430 0.33 +0.4 453,079 + MMP_RS02430 0.47 +1.1
Or see this region's nucleotide sequence