Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02300 and MMP_RS02305 are separated by 38 nucleotides MMP_RS02305 and MMP_RS02310 are separated by 266 nucleotides MMP_RS02310 and MMP_RS02315 are separated by 0 nucleotides MMP_RS02315 and MMP_RS02320 overlap by 14 nucleotides
MMP_RS02300: MMP_RS02300 - NAD(P)H-dependent oxidoreductase, at 433,982 to 434,476
_RS02300
MMP_RS02305: MMP_RS02305 - DUF378 domain-containing protein, at 434,515 to 434,763
_RS02305
MMP_RS02310: MMP_RS02310 - RimK-like ATPgrasp N-terminal domain-containing protein, at 435,030 to 435,635
_RS02310
MMP_RS02315: MMP_RS02315 - RimK family alpha-L-glutamate ligase, at 435,636 to 436,586
_RS02315
MMP_RS02320: MMP_RS02320 - glutamate-cysteine ligase family protein, at 436,573 to 437,676
_RS02320
Position (kb) 435
436 Strain fitness (log2 ratio) -1
0
1 at 434.033 kb on - strand, within MMP_RS02300 at 434.199 kb on + strand, within MMP_RS02300 at 434.207 kb on + strand, within MMP_RS02300 at 434.208 kb on - strand, within MMP_RS02300 at 434.332 kb on - strand, within MMP_RS02300 at 434.449 kb on - strand at 434.449 kb on - strand at 434.456 kb on - strand at 434.457 kb on + strand at 434.457 kb on + strand at 434.474 kb on - strand at 434.532 kb on + strand at 434.535 kb on + strand at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.572 kb on - strand, within MMP_RS02305 at 434.653 kb on + strand, within MMP_RS02305 at 434.665 kb on - strand, within MMP_RS02305 at 434.679 kb on - strand, within MMP_RS02305 at 434.680 kb on + strand, within MMP_RS02305 at 434.680 kb on + strand, within MMP_RS02305 at 434.728 kb on + strand, within MMP_RS02305 at 434.803 kb on - strand at 434.803 kb on - strand at 435.312 kb on - strand, within MMP_RS02310 at 435.922 kb on + strand, within MMP_RS02315 at 436.008 kb on + strand, within MMP_RS02315 at 436.109 kb on + strand, within MMP_RS02315 at 436.278 kb on - strand, within MMP_RS02315 at 436.326 kb on + strand, within MMP_RS02315 at 436.408 kb on + strand, within MMP_RS02315 at 436.473 kb on - strand, within MMP_RS02315 at 436.491 kb on - strand, within MMP_RS02315 at 436.492 kb on + strand at 436.494 kb on + strand at 436.518 kb on - strand at 436.518 kb on - strand at 436.594 kb on - strand at 436.595 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 434,033 - MMP_RS02300 0.10 +0.3 434,199 + MMP_RS02300 0.44 -0.5 434,207 + MMP_RS02300 0.45 +0.5 434,208 - MMP_RS02300 0.46 +0.4 434,332 - MMP_RS02300 0.71 +0.2 434,449 - +0.4 434,449 - -0.3 434,456 - +0.2 434,457 + +0.6 434,457 + -0.3 434,474 - +0.2 434,532 + -1.2 434,535 + +0.1 434,543 - MMP_RS02305 0.11 -0.1 434,543 - MMP_RS02305 0.11 +0.3 434,543 - MMP_RS02305 0.11 +0.4 434,543 - MMP_RS02305 0.11 -0.1 434,543 - MMP_RS02305 0.11 +1.0 434,543 - MMP_RS02305 0.11 +0.0 434,543 - MMP_RS02305 0.11 +0.5 434,543 - MMP_RS02305 0.11 +0.1 434,543 - MMP_RS02305 0.11 +0.4 434,544 + MMP_RS02305 0.12 -0.2 434,544 + MMP_RS02305 0.12 -0.2 434,544 + MMP_RS02305 0.12 -0.3 434,572 - MMP_RS02305 0.23 -0.3 434,653 + MMP_RS02305 0.55 +0.0 434,665 - MMP_RS02305 0.60 +0.2 434,679 - MMP_RS02305 0.66 -0.3 434,680 + MMP_RS02305 0.66 +0.4 434,680 + MMP_RS02305 0.66 -0.3 434,728 + MMP_RS02305 0.86 -0.3 434,803 - +0.4 434,803 - +0.6 435,312 - MMP_RS02310 0.47 -0.7 435,922 + MMP_RS02315 0.30 +0.1 436,008 + MMP_RS02315 0.39 -0.3 436,109 + MMP_RS02315 0.50 -0.3 436,278 - MMP_RS02315 0.68 -0.0 436,326 + MMP_RS02315 0.73 -0.6 436,408 + MMP_RS02315 0.81 +0.1 436,473 - MMP_RS02315 0.88 +0.9 436,491 - MMP_RS02315 0.90 +0.6 436,492 + +0.3 436,494 + +0.0 436,518 - -0.6 436,518 - -0.2 436,594 - +0.1 436,595 + -0.1
Or see this region's nucleotide sequence