Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00390 and MMP_RS00395 are separated by 60 nucleotides MMP_RS00395 and MMP_RS00400 are separated by 253 nucleotides MMP_RS00400 and MMP_RS00405 are separated by 44 nucleotides MMP_RS00405 and MMP_RS00410 are separated by 259 nucleotides MMP_RS00410 and MMP_RS00415 are separated by 67 nucleotides
MMP_RS00390: MMP_RS00390 - ammonium transporter, at 79,353 to 80,588
_RS00390
MMP_RS00395: MMP_RS00395 - MGMT family protein, at 80,649 to 80,948
_RS00395
MMP_RS00400: MMP_RS00400 - tributyrin esterase, at 81,202 to 82,140
_RS00400
MMP_RS00405: MMP_RS00405 - tRNA-Thr, at 82,185 to 82,258
_RS00405
MMP_RS00410: MMP_RS00410 - tRNA-Gln, at 82,518 to 82,593
_RS00410
MMP_RS00415: MMP_RS00415 - DNA primase catalytic subunit PriS, at 82,661 to 83,734
_RS00415
Position (kb) 81
82
83 Strain fitness (log2 ratio) -1
0
1
2 at 80.240 kb on + strand, within MMP_RS00390 at 80.279 kb on - strand, within MMP_RS00390 at 80.311 kb on - strand, within MMP_RS00390 at 80.312 kb on + strand, within MMP_RS00390 at 80.641 kb on - strand at 80.819 kb on + strand, within MMP_RS00395 at 80.952 kb on - strand at 81.070 kb on - strand at 81.075 kb on - strand at 81.086 kb on - strand at 81.087 kb on + strand at 81.089 kb on + strand at 81.122 kb on - strand at 81.161 kb on + strand at 81.164 kb on - strand at 81.181 kb on + strand at 81.599 kb on - strand, within MMP_RS00400 at 81.620 kb on - strand, within MMP_RS00400 at 81.620 kb on - strand, within MMP_RS00400 at 81.914 kb on - strand, within MMP_RS00400 at 82.031 kb on + strand, within MMP_RS00400 at 82.089 kb on - strand at 82.089 kb on - strand at 82.130 kb on - strand at 82.415 kb on - strand at 82.526 kb on + strand at 82.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 80,240 + MMP_RS00390 0.72 -0.1 80,279 - MMP_RS00390 0.75 +0.2 80,311 - MMP_RS00390 0.78 -0.5 80,312 + MMP_RS00390 0.78 -0.1 80,641 - -0.1 80,819 + MMP_RS00395 0.57 -0.3 80,952 - +0.1 81,070 - -0.6 81,075 - -0.6 81,086 - -0.1 81,087 + -0.3 81,089 + -0.2 81,122 - +0.7 81,161 + +0.9 81,164 - -0.2 81,181 + -0.4 81,599 - MMP_RS00400 0.42 -0.5 81,620 - MMP_RS00400 0.45 -0.3 81,620 - MMP_RS00400 0.45 -0.2 81,914 - MMP_RS00400 0.76 -0.0 82,031 + MMP_RS00400 0.88 -0.5 82,089 - +0.1 82,089 - +0.8 82,130 - -0.1 82,415 - +0.5 82,526 + -0.1 82,710 - +2.1
Or see this region's nucleotide sequence