Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2441 and Dshi_2442 are separated by 42 nucleotides Dshi_2442 and Dshi_2443 are separated by 51 nucleotides Dshi_2443 and Dshi_2444 overlap by 4 nucleotides
Dshi_2441: Dshi_2441 - hypothetical protein (RefSeq), at 2,583,868 to 2,584,830
_2441
Dshi_2442: Dshi_2442 - aldehyde dehydrogenase (RefSeq), at 2,584,873 to 2,586,378
_2442
Dshi_2443: Dshi_2443 - 6-phosphogluconate dehydrogenase NAD-binding (RefSeq), at 2,586,430 to 2,587,299
_2443
Dshi_2444: Dshi_2444 - 4-hydroxythreonine-4-phosphate dehydrogenase (RefSeq), at 2,587,296 to 2,588,300
_2444
Position (kb)
2584
2585
2586
2587 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2583.873 kb on + strand at 2583.879 kb on + strand at 2583.968 kb on + strand, within Dshi_2441 at 2583.968 kb on + strand, within Dshi_2441 at 2584.015 kb on - strand, within Dshi_2441 at 2584.077 kb on + strand, within Dshi_2441 at 2584.124 kb on - strand, within Dshi_2441 at 2584.139 kb on - strand, within Dshi_2441 at 2584.200 kb on + strand, within Dshi_2441 at 2584.263 kb on - strand, within Dshi_2441 at 2584.294 kb on - strand, within Dshi_2441 at 2584.309 kb on - strand, within Dshi_2441 at 2584.309 kb on - strand, within Dshi_2441 at 2584.358 kb on - strand, within Dshi_2441 at 2584.360 kb on + strand, within Dshi_2441 at 2584.365 kb on + strand, within Dshi_2441 at 2584.460 kb on + strand, within Dshi_2441 at 2584.468 kb on - strand, within Dshi_2441 at 2584.550 kb on - strand, within Dshi_2441 at 2584.555 kb on - strand, within Dshi_2441 at 2584.571 kb on - strand, within Dshi_2441 at 2584.652 kb on + strand at 2584.659 kb on - strand, within Dshi_2441 at 2584.660 kb on - strand, within Dshi_2441 at 2584.793 kb on + strand at 2584.939 kb on + strand at 2584.996 kb on + strand at 2585.004 kb on - strand at 2585.027 kb on - strand, within Dshi_2442 at 2585.061 kb on - strand, within Dshi_2442 at 2585.092 kb on + strand, within Dshi_2442 at 2585.139 kb on - strand, within Dshi_2442 at 2585.278 kb on - strand, within Dshi_2442 at 2585.321 kb on - strand, within Dshi_2442 at 2585.329 kb on - strand, within Dshi_2442 at 2585.410 kb on - strand, within Dshi_2442 at 2585.527 kb on + strand, within Dshi_2442 at 2585.740 kb on + strand, within Dshi_2442 at 2585.820 kb on + strand, within Dshi_2442 at 2585.934 kb on + strand, within Dshi_2442 at 2586.091 kb on - strand, within Dshi_2442 at 2586.094 kb on + strand, within Dshi_2442 at 2586.103 kb on - strand, within Dshi_2442 at 2586.244 kb on + strand at 2586.256 kb on - strand at 2586.324 kb on - strand at 2586.427 kb on + strand at 2586.535 kb on + strand, within Dshi_2443 at 2586.543 kb on - strand, within Dshi_2443 at 2586.543 kb on - strand, within Dshi_2443 at 2586.579 kb on - strand, within Dshi_2443 at 2586.611 kb on + strand, within Dshi_2443 at 2586.710 kb on + strand, within Dshi_2443 at 2586.750 kb on + strand, within Dshi_2443 at 2586.758 kb on - strand, within Dshi_2443 at 2586.763 kb on - strand, within Dshi_2443 at 2586.894 kb on - strand, within Dshi_2443 at 2586.992 kb on + strand, within Dshi_2443 at 2586.995 kb on - strand, within Dshi_2443 at 2586.998 kb on + strand, within Dshi_2443 at 2587.033 kb on - strand, within Dshi_2443 at 2587.091 kb on - strand, within Dshi_2443 at 2587.330 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,583,873 + -0.3 2,583,879 + +0.8 2,583,968 + Dshi_2441 0.10 +0.2 2,583,968 + Dshi_2441 0.10 -0.2 2,584,015 - Dshi_2441 0.15 -0.8 2,584,077 + Dshi_2441 0.22 +0.0 2,584,124 - Dshi_2441 0.27 +0.5 2,584,139 - Dshi_2441 0.28 +0.6 2,584,200 + Dshi_2441 0.34 -2.2 2,584,263 - Dshi_2441 0.41 -0.5 2,584,294 - Dshi_2441 0.44 +0.5 2,584,309 - Dshi_2441 0.46 -0.2 2,584,309 - Dshi_2441 0.46 -0.4 2,584,358 - Dshi_2441 0.51 -0.5 2,584,360 + Dshi_2441 0.51 +2.8 2,584,365 + Dshi_2441 0.52 +0.3 2,584,460 + Dshi_2441 0.61 -0.8 2,584,468 - Dshi_2441 0.62 +1.0 2,584,550 - Dshi_2441 0.71 +0.8 2,584,555 - Dshi_2441 0.71 +1.1 2,584,571 - Dshi_2441 0.73 +0.5 2,584,652 + +0.5 2,584,659 - Dshi_2441 0.82 -0.1 2,584,660 - Dshi_2441 0.82 -0.7 2,584,793 + +0.2 2,584,939 + +0.0 2,584,996 + +1.3 2,585,004 - -0.7 2,585,027 - Dshi_2442 0.10 -0.2 2,585,061 - Dshi_2442 0.12 -0.3 2,585,092 + Dshi_2442 0.15 -0.0 2,585,139 - Dshi_2442 0.18 -0.3 2,585,278 - Dshi_2442 0.27 -0.6 2,585,321 - Dshi_2442 0.30 +0.4 2,585,329 - Dshi_2442 0.30 -0.1 2,585,410 - Dshi_2442 0.36 +0.5 2,585,527 + Dshi_2442 0.43 +0.6 2,585,740 + Dshi_2442 0.58 +0.9 2,585,820 + Dshi_2442 0.63 -0.6 2,585,934 + Dshi_2442 0.70 -0.4 2,586,091 - Dshi_2442 0.81 +0.4 2,586,094 + Dshi_2442 0.81 -1.5 2,586,103 - Dshi_2442 0.82 -2.2 2,586,244 + +1.3 2,586,256 - +0.2 2,586,324 - -0.6 2,586,427 + -0.3 2,586,535 + Dshi_2443 0.12 +0.1 2,586,543 - Dshi_2443 0.13 -0.2 2,586,543 - Dshi_2443 0.13 -0.0 2,586,579 - Dshi_2443 0.17 +0.5 2,586,611 + Dshi_2443 0.21 +0.1 2,586,710 + Dshi_2443 0.32 -0.3 2,586,750 + Dshi_2443 0.37 -0.9 2,586,758 - Dshi_2443 0.38 +0.0 2,586,763 - Dshi_2443 0.38 -0.1 2,586,894 - Dshi_2443 0.53 +1.4 2,586,992 + Dshi_2443 0.65 +0.8 2,586,995 - Dshi_2443 0.65 -2.1 2,586,998 + Dshi_2443 0.65 -0.6 2,587,033 - Dshi_2443 0.69 +1.4 2,587,091 - Dshi_2443 0.76 -0.4 2,587,330 + +0.5
Or see this region's nucleotide sequence