Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1639 and Dshi_1640 overlap by 4 nucleotides Dshi_1640 and Dshi_1641 are separated by 12 nucleotides Dshi_1641 and Dshi_1642 are separated by 4 nucleotides Dshi_1642 and Dshi_1643 are separated by 144 nucleotides
Dshi_1639: Dshi_1639 - hypothetical protein (RefSeq), at 1,700,730 to 1,701,272
_1639
Dshi_1640: Dshi_1640 - hypothetical protein (RefSeq), at 1,701,269 to 1,701,574
_1640
Dshi_1641: Dshi_1641 - protein of unknown function DUF1330 (RefSeq), at 1,701,587 to 1,701,880
_1641
Dshi_1642: Dshi_1642 - alanyl-tRNA synthetase (RefSeq), at 1,701,885 to 1,704,548
_1642
Dshi_1643: Dshi_1643 - recA protein (RefSeq), at 1,704,693 to 1,705,760
_1643
Position (kb)
1701
1702
1703
1704
1705 Strain fitness (log2 ratio)
-2
-1
0
1 at 1700.922 kb on - strand, within Dshi_1639 at 1701.132 kb on + strand, within Dshi_1639 at 1701.132 kb on + strand, within Dshi_1639 at 1701.140 kb on - strand, within Dshi_1639 at 1701.140 kb on - strand, within Dshi_1639 at 1701.275 kb on - strand at 1701.515 kb on - strand, within Dshi_1640 at 1701.647 kb on + strand, within Dshi_1641 at 1701.746 kb on - strand, within Dshi_1641 at 1704.602 kb on + strand at 1704.602 kb on + strand at 1704.610 kb on - strand at 1704.610 kb on - strand at 1704.789 kb on - strand at 1704.885 kb on - strand, within Dshi_1643 at 1704.988 kb on + strand, within Dshi_1643 at 1705.105 kb on - strand, within Dshi_1643 at 1705.316 kb on + strand, within Dshi_1643 at 1705.504 kb on - strand, within Dshi_1643 at 1705.520 kb on + strand, within Dshi_1643
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,700,922 - Dshi_1639 0.35 +0.3 1,701,132 + Dshi_1639 0.74 -0.2 1,701,132 + Dshi_1639 0.74 +0.4 1,701,140 - Dshi_1639 0.76 +0.1 1,701,140 - Dshi_1639 0.76 -0.9 1,701,275 - -0.2 1,701,515 - Dshi_1640 0.80 +0.1 1,701,647 + Dshi_1641 0.20 -0.8 1,701,746 - Dshi_1641 0.54 -1.6 1,704,602 + -1.6 1,704,602 + +0.6 1,704,610 - -0.9 1,704,610 - -0.7 1,704,789 - -2.5 1,704,885 - Dshi_1643 0.18 -1.0 1,704,988 + Dshi_1643 0.28 -0.8 1,705,105 - Dshi_1643 0.39 -0.7 1,705,316 + Dshi_1643 0.58 +1.0 1,705,504 - Dshi_1643 0.76 -0.4 1,705,520 + Dshi_1643 0.77 +0.2
Or see this region's nucleotide sequence