Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0678 and Dshi_0679 are separated by 8 nucleotides Dshi_0679 and Dshi_0680 are separated by 119 nucleotides Dshi_0680 and Dshi_0681 overlap by 8 nucleotides Dshi_0681 and Dshi_0682 are separated by 76 nucleotides Dshi_0682 and Dshi_0683 are separated by 176 nucleotides
Dshi_0678: Dshi_0678 - glycosyl transferase family 14 (RefSeq), at 684,684 to 686,261
_0678
Dshi_0679: Dshi_0679 - histidine triad (HIT) protein (RefSeq), at 686,270 to 686,641
_0679
Dshi_0680: Dshi_0680 - phosphoribosylaminoimidazole carboxylase, ATPase subunit (RefSeq), at 686,761 to 687,834
_0680
Dshi_0681: Dshi_0681 - phosphoribosylaminoimidazole carboxylase, catalytic subunit (RefSeq), at 687,827 to 688,312
_0681
Dshi_0682: Dshi_0682 - protein of unknown function DUF465 (RefSeq), at 688,389 to 688,613
_0682
Dshi_0683: Dshi_0683 - heat shock protein Hsp20 (RefSeq), at 688,790 to 689,233
_0683
Position (kb) 686
687
688 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 685.932 kb on + strand, within Dshi_0678 at 686.194 kb on - strand at 686.275 kb on - strand at 686.277 kb on + strand at 686.407 kb on - strand, within Dshi_0679 at 686.420 kb on + strand, within Dshi_0679 at 686.533 kb on + strand, within Dshi_0679 at 686.609 kb on + strand at 686.634 kb on - strand at 687.152 kb on - strand, within Dshi_0680 at 688.374 kb on + strand at 688.492 kb on - strand, within Dshi_0682 at 688.657 kb on + strand at 688.681 kb on - strand at 688.684 kb on + strand at 688.692 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 685,932 + Dshi_0678 0.79 +0.1 686,194 - -0.1 686,275 - +0.8 686,277 + -0.3 686,407 - Dshi_0679 0.37 -0.3 686,420 + Dshi_0679 0.40 -1.1 686,533 + Dshi_0679 0.71 +0.5 686,609 + +0.2 686,634 - +0.0 687,152 - Dshi_0680 0.36 -3.9 688,374 + -0.4 688,492 - Dshi_0682 0.46 -0.0 688,657 + -0.0 688,681 - -0.1 688,684 + -0.5 688,692 - +0.0
Or see this region's nucleotide sequence