Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0537 and Dshi_0538 are separated by 185 nucleotides Dshi_0538 and Dshi_0539 are separated by 13 nucleotides Dshi_0539 and Dshi_0540 are separated by 77 nucleotides Dshi_0540 and Dshi_0541 are separated by 134 nucleotides
Dshi_0537: Dshi_0537 - carbohydrate kinase FGGY (RefSeq), at 522,165 to 523,658
_0537
Dshi_0538: Dshi_0538 - protein of unknown function DUF1045 (RefSeq), at 523,844 to 524,515
_0538
Dshi_0539: Dshi_0539 - aldo/keto reductase (RefSeq), at 524,529 to 525,467
_0539
Dshi_0540: Dshi_0540 - NnrUfamily protein (RefSeq), at 525,545 to 526,321
_0540
Dshi_0541: Dshi_0541 - oxygen-independent coproporphyrinogen III oxidase (RefSeq), at 526,456 to 527,820
_0541
Position (kb) 524
525
526 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 523.544 kb on + strand at 523.544 kb on + strand at 523.552 kb on - strand at 523.552 kb on - strand at 523.574 kb on + strand at 523.584 kb on + strand at 523.597 kb on - strand at 523.609 kb on - strand at 523.640 kb on - strand at 523.752 kb on - strand at 523.753 kb on - strand at 523.754 kb on + strand at 523.762 kb on - strand at 523.766 kb on - strand at 523.813 kb on + strand at 523.813 kb on - strand at 524.540 kb on - strand at 524.572 kb on + strand at 524.577 kb on + strand at 524.595 kb on - strand at 524.704 kb on + strand, within Dshi_0539 at 524.723 kb on - strand, within Dshi_0539 at 524.794 kb on + strand, within Dshi_0539 at 524.915 kb on + strand, within Dshi_0539 at 524.919 kb on - strand, within Dshi_0539 at 524.922 kb on + strand, within Dshi_0539 at 524.928 kb on + strand, within Dshi_0539 at 524.983 kb on - strand, within Dshi_0539 at 524.989 kb on + strand, within Dshi_0539 at 525.016 kb on + strand, within Dshi_0539 at 525.019 kb on - strand, within Dshi_0539 at 525.024 kb on - strand, within Dshi_0539 at 525.102 kb on - strand, within Dshi_0539 at 525.171 kb on - strand, within Dshi_0539 at 525.186 kb on + strand, within Dshi_0539 at 525.194 kb on - strand, within Dshi_0539 at 525.261 kb on + strand, within Dshi_0539 at 525.318 kb on - strand, within Dshi_0539 at 525.341 kb on + strand, within Dshi_0539 at 525.358 kb on + strand, within Dshi_0539 at 525.421 kb on - strand at 525.427 kb on + strand at 525.462 kb on + strand at 525.470 kb on - strand at 525.476 kb on + strand at 525.520 kb on - strand at 525.531 kb on + strand at 525.531 kb on + strand at 525.539 kb on + strand at 525.583 kb on + strand at 525.603 kb on + strand at 525.733 kb on + strand, within Dshi_0540 at 525.780 kb on + strand, within Dshi_0540 at 525.800 kb on + strand, within Dshi_0540 at 525.805 kb on + strand, within Dshi_0540 at 525.848 kb on + strand, within Dshi_0540 at 525.867 kb on + strand, within Dshi_0540 at 525.932 kb on - strand, within Dshi_0540 at 525.985 kb on - strand, within Dshi_0540 at 525.995 kb on - strand, within Dshi_0540 at 526.003 kb on + strand, within Dshi_0540 at 526.065 kb on - strand, within Dshi_0540 at 526.115 kb on + strand, within Dshi_0540 at 526.150 kb on + strand, within Dshi_0540 at 526.235 kb on - strand, within Dshi_0540 at 526.248 kb on + strand at 526.308 kb on + strand at 526.347 kb on + strand at 526.368 kb on + strand at 526.447 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 523,544 + -1.3 523,544 + +0.8 523,552 - +0.1 523,552 - -0.8 523,574 + +0.2 523,584 + -0.7 523,597 - -1.0 523,609 - +1.6 523,640 - +3.4 523,752 - +0.6 523,753 - -0.4 523,754 + -0.2 523,762 - +0.2 523,766 - -0.3 523,813 + -1.5 523,813 - +1.5 524,540 - +1.2 524,572 + +0.6 524,577 + -2.9 524,595 - +0.2 524,704 + Dshi_0539 0.19 +0.2 524,723 - Dshi_0539 0.21 +0.2 524,794 + Dshi_0539 0.28 +0.2 524,915 + Dshi_0539 0.41 +1.4 524,919 - Dshi_0539 0.42 +0.1 524,922 + Dshi_0539 0.42 +1.1 524,928 + Dshi_0539 0.42 -0.3 524,983 - Dshi_0539 0.48 -0.6 524,989 + Dshi_0539 0.49 -0.1 525,016 + Dshi_0539 0.52 -0.3 525,019 - Dshi_0539 0.52 +0.4 525,024 - Dshi_0539 0.53 +0.3 525,102 - Dshi_0539 0.61 -0.7 525,171 - Dshi_0539 0.68 -0.8 525,186 + Dshi_0539 0.70 +0.5 525,194 - Dshi_0539 0.71 +1.5 525,261 + Dshi_0539 0.78 -0.1 525,318 - Dshi_0539 0.84 +0.1 525,341 + Dshi_0539 0.86 +0.2 525,358 + Dshi_0539 0.88 +2.4 525,421 - +0.6 525,427 + -0.9 525,462 + +1.5 525,470 - +0.7 525,476 + +1.3 525,520 - -0.3 525,531 + -0.4 525,531 + -0.9 525,539 + -1.1 525,583 + +0.3 525,603 + -1.2 525,733 + Dshi_0540 0.24 -1.1 525,780 + Dshi_0540 0.30 +1.1 525,800 + Dshi_0540 0.33 -0.1 525,805 + Dshi_0540 0.33 -0.2 525,848 + Dshi_0540 0.39 +0.2 525,867 + Dshi_0540 0.41 +0.8 525,932 - Dshi_0540 0.50 -0.1 525,985 - Dshi_0540 0.57 +0.5 525,995 - Dshi_0540 0.58 +0.5 526,003 + Dshi_0540 0.59 +0.6 526,065 - Dshi_0540 0.67 +0.7 526,115 + Dshi_0540 0.73 +0.8 526,150 + Dshi_0540 0.78 +1.5 526,235 - Dshi_0540 0.89 -0.7 526,248 + +0.1 526,308 + -0.3 526,347 + -1.4 526,368 + +1.0 526,447 - +0.1
Or see this region's nucleotide sequence