Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0537 and Dshi_0538 are separated by 185 nucleotides Dshi_0538 and Dshi_0539 are separated by 13 nucleotides
Dshi_0537: Dshi_0537 - carbohydrate kinase FGGY (RefSeq), at 522,165 to 523,658
_0537
Dshi_0538: Dshi_0538 - protein of unknown function DUF1045 (RefSeq), at 523,844 to 524,515
_0538
Dshi_0539: Dshi_0539 - aldo/keto reductase (RefSeq), at 524,529 to 525,467
_0539
Position (kb)
523
524
525 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 522.869 kb on + strand, within Dshi_0537 at 522.869 kb on + strand, within Dshi_0537 at 522.916 kb on + strand, within Dshi_0537 at 522.936 kb on + strand, within Dshi_0537 at 522.942 kb on - strand, within Dshi_0537 at 522.944 kb on - strand, within Dshi_0537 at 522.944 kb on - strand, within Dshi_0537 at 523.027 kb on - strand, within Dshi_0537 at 523.106 kb on + strand, within Dshi_0537 at 523.142 kb on + strand, within Dshi_0537 at 523.176 kb on - strand, within Dshi_0537 at 523.215 kb on + strand, within Dshi_0537 at 523.215 kb on + strand, within Dshi_0537 at 523.223 kb on - strand, within Dshi_0537 at 523.223 kb on - strand, within Dshi_0537 at 523.223 kb on - strand, within Dshi_0537 at 523.274 kb on + strand, within Dshi_0537 at 523.303 kb on - strand, within Dshi_0537 at 523.393 kb on + strand, within Dshi_0537 at 523.393 kb on + strand, within Dshi_0537 at 523.544 kb on + strand at 523.544 kb on + strand at 523.552 kb on - strand at 523.552 kb on - strand at 523.574 kb on + strand at 523.584 kb on + strand at 523.597 kb on - strand at 523.609 kb on - strand at 523.640 kb on - strand at 523.752 kb on - strand at 523.753 kb on - strand at 523.754 kb on + strand at 523.762 kb on - strand at 523.766 kb on - strand at 523.813 kb on + strand at 523.813 kb on - strand at 524.540 kb on - strand at 524.572 kb on + strand at 524.577 kb on + strand at 524.595 kb on - strand at 524.704 kb on + strand, within Dshi_0539 at 524.723 kb on - strand, within Dshi_0539 at 524.794 kb on + strand, within Dshi_0539 at 524.915 kb on + strand, within Dshi_0539 at 524.919 kb on - strand, within Dshi_0539 at 524.922 kb on + strand, within Dshi_0539 at 524.928 kb on + strand, within Dshi_0539 at 524.983 kb on - strand, within Dshi_0539 at 524.989 kb on + strand, within Dshi_0539 at 525.016 kb on + strand, within Dshi_0539 at 525.019 kb on - strand, within Dshi_0539 at 525.024 kb on - strand, within Dshi_0539 at 525.102 kb on - strand, within Dshi_0539 at 525.171 kb on - strand, within Dshi_0539 at 525.186 kb on + strand, within Dshi_0539 at 525.194 kb on - strand, within Dshi_0539 at 525.261 kb on + strand, within Dshi_0539 at 525.318 kb on - strand, within Dshi_0539 at 525.341 kb on + strand, within Dshi_0539 at 525.358 kb on + strand, within Dshi_0539 at 525.421 kb on - strand at 525.427 kb on + strand at 525.462 kb on + strand at 525.470 kb on - strand at 525.476 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 522,869 + Dshi_0537 0.47 +1.0 522,869 + Dshi_0537 0.47 -1.1 522,916 + Dshi_0537 0.50 -1.6 522,936 + Dshi_0537 0.52 -0.3 522,942 - Dshi_0537 0.52 -0.1 522,944 - Dshi_0537 0.52 +0.6 522,944 - Dshi_0537 0.52 +1.1 523,027 - Dshi_0537 0.58 +0.4 523,106 + Dshi_0537 0.63 -1.0 523,142 + Dshi_0537 0.65 -0.2 523,176 - Dshi_0537 0.68 -0.3 523,215 + Dshi_0537 0.70 -2.0 523,215 + Dshi_0537 0.70 -1.3 523,223 - Dshi_0537 0.71 -2.6 523,223 - Dshi_0537 0.71 +1.3 523,223 - Dshi_0537 0.71 -0.6 523,274 + Dshi_0537 0.74 -0.4 523,303 - Dshi_0537 0.76 +0.5 523,393 + Dshi_0537 0.82 -0.3 523,393 + Dshi_0537 0.82 -0.9 523,544 + -1.3 523,544 + +0.8 523,552 - +0.1 523,552 - -0.8 523,574 + +0.2 523,584 + -0.7 523,597 - -1.0 523,609 - +1.6 523,640 - +3.4 523,752 - +0.6 523,753 - -0.4 523,754 + -0.2 523,762 - +0.2 523,766 - -0.3 523,813 + -1.5 523,813 - +1.5 524,540 - +1.2 524,572 + +0.6 524,577 + -2.9 524,595 - +0.2 524,704 + Dshi_0539 0.19 +0.2 524,723 - Dshi_0539 0.21 +0.2 524,794 + Dshi_0539 0.28 +0.2 524,915 + Dshi_0539 0.41 +1.4 524,919 - Dshi_0539 0.42 +0.1 524,922 + Dshi_0539 0.42 +1.1 524,928 + Dshi_0539 0.42 -0.3 524,983 - Dshi_0539 0.48 -0.6 524,989 + Dshi_0539 0.49 -0.1 525,016 + Dshi_0539 0.52 -0.3 525,019 - Dshi_0539 0.52 +0.4 525,024 - Dshi_0539 0.53 +0.3 525,102 - Dshi_0539 0.61 -0.7 525,171 - Dshi_0539 0.68 -0.8 525,186 + Dshi_0539 0.70 +0.5 525,194 - Dshi_0539 0.71 +1.5 525,261 + Dshi_0539 0.78 -0.1 525,318 - Dshi_0539 0.84 +0.1 525,341 + Dshi_0539 0.86 +0.2 525,358 + Dshi_0539 0.88 +2.4 525,421 - +0.6 525,427 + -0.9 525,462 + +1.5 525,470 - +0.7 525,476 + +1.3
Or see this region's nucleotide sequence