Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Met-4 and tRNA-Met-3 are separated by 147 nucleotides tRNA-Met-3 and tRNA-Met-2 are separated by 147 nucleotides tRNA-Met-2 and tRNA-Met-1 are separated by 37 nucleotides tRNA-Met-1 and SO1851 are separated by 257 nucleotides SO1851 and SO1852 overlap by 14 nucleotides SO1852 and SO1853 are separated by 1 nucleotides
SO_t027: tRNA-Met-4 - tRNA-Met (RefSeq), at 1,939,976 to 1,940,052
tRNA-Met-4
SO_t028: tRNA-Met-3 - tRNA-Met (RefSeq), at 1,940,200 to 1,940,276
tRNA-Met-3
SO_t029: tRNA-Met-2 - tRNA-Met (RefSeq), at 1,940,424 to 1,940,500
tRNA-Met-2
SO_t030: tRNA-Met-1 - tRNA-Met (RefSeq), at 1,940,538 to 1,940,614
tRNA-Met-1
SO1851: SO1851 - conserved hypothetical protein (NCBI ptt file), at 1,940,872 to 1,943,007
SO1851
SO1852: SO1852 - conserved hypothetical protein (NCBI ptt file), at 1,942,994 to 1,943,239
SO1852
SO1853: SO1853 - ABC transporter, ATP-binding protein (NCBI ptt file), at 1,943,241 to 1,945,163
SO1853
Position (kb)
1940
1941
1942
1943
1944 Strain fitness (log2 ratio)
-1
0
1
2 at 1940.152 kb on + strand at 1940.223 kb on - strand, within tRNA-Met-3 at 1940.339 kb on + strand at 1940.635 kb on + strand at 1940.643 kb on - strand at 1940.854 kb on - strand at 1940.989 kb on + strand at 1940.989 kb on + strand at 1940.991 kb on + strand at 1940.997 kb on - strand at 1940.997 kb on - strand at 1940.997 kb on - strand at 1940.997 kb on - strand at 1940.997 kb on - strand at 1941.008 kb on + strand at 1941.008 kb on + strand at 1941.008 kb on + strand at 1941.016 kb on - strand at 1941.025 kb on + strand at 1941.025 kb on + strand at 1941.038 kb on + strand at 1941.046 kb on - strand at 1941.104 kb on + strand, within SO1851 at 1941.243 kb on - strand, within SO1851 at 1941.256 kb on + strand, within SO1851 at 1941.318 kb on - strand, within SO1851 at 1941.363 kb on + strand, within SO1851 at 1941.417 kb on + strand, within SO1851 at 1941.427 kb on + strand, within SO1851 at 1941.476 kb on + strand, within SO1851 at 1941.484 kb on - strand, within SO1851 at 1941.484 kb on - strand, within SO1851 at 1941.484 kb on - strand, within SO1851 at 1941.489 kb on - strand, within SO1851 at 1941.567 kb on + strand, within SO1851 at 1941.631 kb on - strand, within SO1851 at 1941.737 kb on - strand, within SO1851 at 1941.760 kb on - strand, within SO1851 at 1941.775 kb on + strand, within SO1851 at 1941.775 kb on + strand, within SO1851 at 1941.775 kb on + strand, within SO1851 at 1941.796 kb on + strand, within SO1851 at 1941.808 kb on + strand, within SO1851 at 1941.821 kb on + strand, within SO1851 at 1941.826 kb on - strand, within SO1851 at 1941.900 kb on + strand, within SO1851 at 1941.960 kb on + strand, within SO1851 at 1942.157 kb on - strand, within SO1851 at 1942.395 kb on + strand, within SO1851 at 1942.437 kb on + strand, within SO1851 at 1942.448 kb on + strand, within SO1851 at 1942.538 kb on - strand, within SO1851 at 1942.540 kb on + strand, within SO1851 at 1942.548 kb on - strand, within SO1851 at 1942.569 kb on - strand, within SO1851 at 1942.569 kb on - strand, within SO1851 at 1942.569 kb on - strand, within SO1851 at 1942.685 kb on - strand, within SO1851 at 1942.775 kb on + strand, within SO1851 at 1942.835 kb on - strand at 1942.871 kb on + strand at 1942.915 kb on + strand at 1943.022 kb on + strand, within SO1852 at 1943.022 kb on + strand, within SO1852 at 1943.022 kb on + strand, within SO1852 at 1943.085 kb on - strand, within SO1852 at 1943.138 kb on - strand, within SO1852 at 1943.140 kb on + strand, within SO1852 at 1943.148 kb on - strand, within SO1852 at 1943.279 kb on + strand at 1943.341 kb on - strand at 1943.348 kb on + strand at 1943.538 kb on - strand, within SO1853 at 1943.538 kb on - strand, within SO1853 at 1943.538 kb on - strand, within SO1853 at 1943.626 kb on + strand, within SO1853 at 1943.636 kb on + strand, within SO1853 at 1943.705 kb on + strand, within SO1853 at 1943.821 kb on + strand, within SO1853 at 1943.882 kb on + strand, within SO1853 at 1943.937 kb on + strand, within SO1853
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 1,940,152 + +0.2 1,940,223 - tRNA-Met-3 SO_t028 0.30 -0.2 1,940,339 + +0.9 1,940,635 + +0.9 1,940,643 - -0.2 1,940,854 - +0.5 1,940,989 + +0.4 1,940,989 + -0.3 1,940,991 + -0.7 1,940,997 - +1.5 1,940,997 - +0.6 1,940,997 - -0.1 1,940,997 - -0.2 1,940,997 - -0.1 1,941,008 + -0.1 1,941,008 + +0.2 1,941,008 + -0.5 1,941,016 - +0.0 1,941,025 + -0.7 1,941,025 + -0.2 1,941,038 + -0.4 1,941,046 - -0.1 1,941,104 + SO1851 0.11 +1.1 1,941,243 - SO1851 0.17 -0.1 1,941,256 + SO1851 0.18 +0.2 1,941,318 - SO1851 0.21 -0.6 1,941,363 + SO1851 0.23 -0.8 1,941,417 + SO1851 0.26 -0.8 1,941,427 + SO1851 0.26 +2.1 1,941,476 + SO1851 0.28 +0.1 1,941,484 - SO1851 0.29 -0.3 1,941,484 - SO1851 0.29 +1.4 1,941,484 - SO1851 0.29 +1.0 1,941,489 - SO1851 0.29 -0.4 1,941,567 + SO1851 0.33 +0.3 1,941,631 - SO1851 0.36 -0.8 1,941,737 - SO1851 0.40 -1.5 1,941,760 - SO1851 0.42 +0.1 1,941,775 + SO1851 0.42 -0.4 1,941,775 + SO1851 0.42 +0.3 1,941,775 + SO1851 0.42 +0.3 1,941,796 + SO1851 0.43 +0.1 1,941,808 + SO1851 0.44 +0.5 1,941,821 + SO1851 0.44 -0.2 1,941,826 - SO1851 0.45 -0.4 1,941,900 + SO1851 0.48 +0.3 1,941,960 + SO1851 0.51 +0.5 1,942,157 - SO1851 0.60 -1.1 1,942,395 + SO1851 0.71 -1.4 1,942,437 + SO1851 0.73 -1.1 1,942,448 + SO1851 0.74 -0.3 1,942,538 - SO1851 0.78 -0.0 1,942,540 + SO1851 0.78 +0.0 1,942,548 - SO1851 0.78 -0.7 1,942,569 - SO1851 0.79 +0.6 1,942,569 - SO1851 0.79 -0.9 1,942,569 - SO1851 0.79 -0.1 1,942,685 - SO1851 0.85 +0.1 1,942,775 + SO1851 0.89 -1.3 1,942,835 - -1.0 1,942,871 + -0.3 1,942,915 + -0.4 1,943,022 + SO1852 0.11 -1.5 1,943,022 + SO1852 0.11 -0.3 1,943,022 + SO1852 0.11 -0.9 1,943,085 - SO1852 0.37 -0.9 1,943,138 - SO1852 0.59 -0.1 1,943,140 + SO1852 0.59 -0.4 1,943,148 - SO1852 0.63 -0.3 1,943,279 + -0.6 1,943,341 - +0.8 1,943,348 + +0.7 1,943,538 - SO1853 0.15 -0.6 1,943,538 - SO1853 0.15 +0.2 1,943,538 - SO1853 0.15 -0.1 1,943,626 + SO1853 0.20 +0.6 1,943,636 + SO1853 0.21 +0.2 1,943,705 + SO1853 0.24 -0.4 1,943,821 + SO1853 0.30 +0.8 1,943,882 + SO1853 0.33 -0.5 1,943,937 + SO1853 0.36 +0.3
Or see this region's nucleotide sequence