Experiment: m.b. Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_220 and HP15_222 overlap by 18 nucleotides HP15_222 and HP15_221 are separated by 42 nucleotides HP15_221 and HP15_223 are separated by 95 nucleotides HP15_223 and HP15_224 are separated by 124 nucleotides HP15_224 and HP15_225 overlap by 4 nucleotides
HP15_220: HP15_220 - disulfide bond formation protein B, at 208,222 to 208,629
_220
HP15_222: HP15_222 - hypothetical protein, at 208,612 to 208,728
_222
HP15_221: HP15_221 - anti-RNA polymerase sigma 70 factor, at 208,771 to 209,250
_221
HP15_223: HP15_223 - peptidylprolyl isomerase, FKBP-type, at 209,346 to 210,062
_223
HP15_224: HP15_224 - conserved hypothetical protein, secreted, at 210,187 to 210,573
_224
HP15_225: HP15_225 - conserved hypothetical protein, at 210,570 to 211,127
_225
Position (kb) 209
210
211 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 208.444 kb on - strand, within HP15_220 at 208.486 kb on - strand, within HP15_220 at 208.505 kb on - strand, within HP15_220 at 208.564 kb on + strand, within HP15_220 at 208.572 kb on - strand, within HP15_220 at 208.811 kb on + strand at 208.835 kb on + strand, within HP15_221 at 208.868 kb on + strand, within HP15_221 at 209.032 kb on - strand, within HP15_221 at 209.368 kb on + strand at 209.397 kb on + strand at 209.402 kb on + strand at 209.405 kb on - strand at 209.543 kb on - strand, within HP15_223 at 209.789 kb on + strand, within HP15_223 at 210.005 kb on + strand at 210.090 kb on + strand at 210.098 kb on - strand at 210.107 kb on + strand at 210.150 kb on - strand at 210.238 kb on + strand, within HP15_224 at 210.345 kb on + strand, within HP15_224 at 210.345 kb on + strand, within HP15_224 at 210.350 kb on + strand, within HP15_224 at 210.464 kb on - strand, within HP15_224 at 210.486 kb on + strand, within HP15_224 at 210.494 kb on - strand, within HP15_224 at 210.494 kb on - strand, within HP15_224 at 210.650 kb on - strand, within HP15_225 at 210.727 kb on - strand, within HP15_225 at 210.758 kb on - strand, within HP15_225 at 210.817 kb on - strand, within HP15_225 at 210.821 kb on + strand, within HP15_225 at 210.831 kb on + strand, within HP15_225 at 210.866 kb on + strand, within HP15_225 at 210.895 kb on - strand, within HP15_225 at 210.918 kb on + strand, within HP15_225 at 210.940 kb on - strand, within HP15_225 at 210.999 kb on + strand, within HP15_225 at 210.999 kb on + strand, within HP15_225 at 210.999 kb on + strand, within HP15_225 at 211.026 kb on + strand, within HP15_225 at 211.048 kb on + strand, within HP15_225
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloramphenicol 0.0002 mg/ml remove 208,444 - HP15_220 0.54 -3.9 208,486 - HP15_220 0.65 -2.2 208,505 - HP15_220 0.69 -1.8 208,564 + HP15_220 0.84 -3.8 208,572 - HP15_220 0.86 -1.4 208,811 + +0.7 208,835 + HP15_221 0.13 +0.1 208,868 + HP15_221 0.20 -1.1 209,032 - HP15_221 0.54 +0.4 209,368 + -0.2 209,397 + -2.2 209,402 + -2.8 209,405 - -0.2 209,543 - HP15_223 0.27 -2.0 209,789 + HP15_223 0.62 -0.3 210,005 + -1.0 210,090 + +0.9 210,098 - -0.8 210,107 + +0.0 210,150 - +0.3 210,238 + HP15_224 0.13 +1.6 210,345 + HP15_224 0.41 +0.1 210,345 + HP15_224 0.41 +0.9 210,350 + HP15_224 0.42 -0.2 210,464 - HP15_224 0.72 +0.3 210,486 + HP15_224 0.77 -0.3 210,494 - HP15_224 0.79 +2.0 210,494 - HP15_224 0.79 +0.4 210,650 - HP15_225 0.14 +0.3 210,727 - HP15_225 0.28 -0.2 210,758 - HP15_225 0.34 +0.0 210,817 - HP15_225 0.44 +2.5 210,821 + HP15_225 0.45 +0.3 210,831 + HP15_225 0.47 +1.5 210,866 + HP15_225 0.53 +1.1 210,895 - HP15_225 0.58 +0.4 210,918 + HP15_225 0.62 +0.7 210,940 - HP15_225 0.66 -0.2 210,999 + HP15_225 0.77 +1.4 210,999 + HP15_225 0.77 +0.2 210,999 + HP15_225 0.77 +1.2 211,026 + HP15_225 0.82 +1.5 211,048 + HP15_225 0.86 +3.6
Or see this region's nucleotide sequence