Experiment: Gelatin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysA and SO4310 are separated by 42 nucleotides SO4310 and cyaY are separated by 73 nucleotides cyaY and SO4312 are separated by 40 nucleotides SO4312 and hemC are separated by 201 nucleotides
SO4309: lysA - diaminopimelate decarboxylase (NCBI ptt file), at 4,486,089 to 4,487,333
lysA
SO4310: SO4310 - hypothetical protein (NCBI ptt file), at 4,487,376 to 4,487,570
SO4310
SO4311: cyaY - cyay protein (NCBI ptt file), at 4,487,644 to 4,487,967
cyaY
SO4312: SO4312 - adenylate cyclase CyaA, putative (NCBI ptt file), at 4,488,008 to 4,490,422
SO4312
SO4313: hemC - porphobilinogen deaminase (NCBI ptt file), at 4,490,624 to 4,491,556
hemC
Position (kb)
4488
4489
4490
4491 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4487.013 kb on + strand, within lysA at 4487.014 kb on + strand, within lysA at 4487.115 kb on - strand, within lysA at 4487.117 kb on - strand, within lysA at 4487.126 kb on - strand, within lysA at 4487.140 kb on - strand, within lysA at 4487.142 kb on + strand, within lysA at 4487.169 kb on - strand, within lysA at 4487.169 kb on - strand, within lysA at 4487.199 kb on + strand, within lysA at 4487.244 kb on + strand at 4487.262 kb on + strand at 4487.265 kb on - strand at 4487.265 kb on - strand at 4487.290 kb on - strand at 4487.291 kb on + strand at 4487.323 kb on + strand at 4487.406 kb on - strand, within SO4310 at 4487.413 kb on - strand, within SO4310 at 4487.436 kb on - strand, within SO4310 at 4487.436 kb on - strand, within SO4310 at 4487.470 kb on - strand, within SO4310 at 4487.482 kb on + strand, within SO4310 at 4487.482 kb on + strand, within SO4310 at 4487.490 kb on - strand, within SO4310 at 4487.490 kb on - strand, within SO4310 at 4487.576 kb on - strand at 4487.874 kb on + strand, within cyaY at 4487.882 kb on + strand, within cyaY at 4487.890 kb on - strand, within cyaY at 4487.933 kb on - strand, within cyaY at 4487.948 kb on + strand at 4487.960 kb on + strand at 4487.964 kb on + strand at 4487.968 kb on - strand at 4487.985 kb on - strand at 4488.029 kb on + strand at 4488.029 kb on + strand at 4488.037 kb on - strand at 4488.037 kb on - strand at 4488.049 kb on + strand at 4488.049 kb on + strand at 4488.050 kb on - strand at 4488.335 kb on - strand, within SO4312 at 4488.339 kb on + strand, within SO4312 at 4488.365 kb on - strand, within SO4312 at 4488.399 kb on + strand, within SO4312 at 4488.407 kb on - strand, within SO4312 at 4488.472 kb on + strand, within SO4312 at 4488.472 kb on + strand, within SO4312 at 4488.472 kb on + strand, within SO4312 at 4488.472 kb on + strand, within SO4312 at 4488.480 kb on - strand, within SO4312 at 4488.480 kb on - strand, within SO4312 at 4488.480 kb on - strand, within SO4312 at 4488.501 kb on - strand, within SO4312 at 4488.504 kb on - strand, within SO4312 at 4488.570 kb on - strand, within SO4312 at 4488.570 kb on - strand, within SO4312 at 4488.619 kb on - strand, within SO4312 at 4488.619 kb on - strand, within SO4312 at 4488.685 kb on + strand, within SO4312 at 4488.806 kb on + strand, within SO4312 at 4488.858 kb on + strand, within SO4312 at 4488.858 kb on + strand, within SO4312 at 4488.858 kb on + strand, within SO4312 at 4488.858 kb on + strand, within SO4312 at 4488.866 kb on - strand, within SO4312 at 4488.866 kb on - strand, within SO4312 at 4488.866 kb on - strand, within SO4312 at 4488.868 kb on + strand, within SO4312 at 4488.868 kb on + strand, within SO4312 at 4488.868 kb on + strand, within SO4312 at 4488.868 kb on + strand, within SO4312 at 4488.868 kb on + strand, within SO4312 at 4488.868 kb on + strand, within SO4312 at 4488.874 kb on + strand, within SO4312 at 4488.876 kb on - strand, within SO4312 at 4488.876 kb on - strand at 4488.924 kb on - strand, within SO4312 at 4488.948 kb on + strand, within SO4312 at 4488.948 kb on + strand, within SO4312 at 4488.958 kb on + strand, within SO4312 at 4488.984 kb on + strand, within SO4312 at 4489.029 kb on - strand, within SO4312 at 4489.030 kb on + strand, within SO4312 at 4489.075 kb on + strand, within SO4312 at 4489.075 kb on + strand, within SO4312 at 4489.075 kb on + strand, within SO4312 at 4489.131 kb on - strand, within SO4312 at 4489.195 kb on - strand, within SO4312 at 4489.224 kb on - strand, within SO4312 at 4489.231 kb on - strand, within SO4312 at 4489.313 kb on - strand, within SO4312 at 4489.317 kb on + strand, within SO4312 at 4489.357 kb on + strand, within SO4312 at 4489.453 kb on - strand, within SO4312 at 4489.453 kb on - strand, within SO4312 at 4489.467 kb on + strand, within SO4312 at 4489.467 kb on + strand, within SO4312 at 4489.497 kb on - strand, within SO4312 at 4489.532 kb on - strand, within SO4312 at 4489.663 kb on + strand, within SO4312 at 4489.726 kb on - strand, within SO4312 at 4489.740 kb on + strand, within SO4312 at 4489.800 kb on - strand, within SO4312 at 4489.805 kb on - strand, within SO4312 at 4489.901 kb on - strand, within SO4312 at 4490.010 kb on - strand, within SO4312 at 4490.113 kb on + strand, within SO4312 at 4490.113 kb on + strand, within SO4312 at 4490.139 kb on + strand, within SO4312 at 4490.148 kb on + strand, within SO4312 at 4490.207 kb on + strand at 4490.207 kb on + strand at 4490.247 kb on - strand at 4490.345 kb on + strand at 4490.352 kb on + strand at 4490.359 kb on - strand at 4490.422 kb on + strand at 4490.430 kb on - strand at 4490.453 kb on - strand at 4490.521 kb on + strand at 4490.529 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gelatin (C) remove 4,487,013 + lysA SO4309 0.74 -0.5 4,487,014 + lysA SO4309 0.74 -0.2 4,487,115 - lysA SO4309 0.82 -1.9 4,487,117 - lysA SO4309 0.83 -1.2 4,487,126 - lysA SO4309 0.83 -1.2 4,487,140 - lysA SO4309 0.84 -1.8 4,487,142 + lysA SO4309 0.85 -1.4 4,487,169 - lysA SO4309 0.87 -0.3 4,487,169 - lysA SO4309 0.87 -1.2 4,487,199 + lysA SO4309 0.89 -1.7 4,487,244 + -1.4 4,487,262 + -2.9 4,487,265 - +1.3 4,487,265 - -0.5 4,487,290 - -2.7 4,487,291 + -1.5 4,487,323 + -1.3 4,487,406 - SO4310 0.15 -0.4 4,487,413 - SO4310 0.19 +0.4 4,487,436 - SO4310 0.31 +0.3 4,487,436 - SO4310 0.31 +1.6 4,487,470 - SO4310 0.48 +0.5 4,487,482 + SO4310 0.54 +1.3 4,487,482 + SO4310 0.54 -0.2 4,487,490 - SO4310 0.58 +0.5 4,487,490 - SO4310 0.58 +0.2 4,487,576 - +0.2 4,487,874 + cyaY SO4311 0.71 -1.2 4,487,882 + cyaY SO4311 0.73 -0.8 4,487,890 - cyaY SO4311 0.76 -0.7 4,487,933 - cyaY SO4311 0.89 -1.6 4,487,948 + +0.4 4,487,960 + -0.2 4,487,964 + +0.3 4,487,968 - +0.1 4,487,985 - +0.6 4,488,029 + +1.6 4,488,029 + +1.6 4,488,037 - +1.7 4,488,037 - +1.6 4,488,049 + +0.8 4,488,049 + +1.2 4,488,050 - +1.7 4,488,335 - SO4312 0.14 +1.0 4,488,339 + SO4312 0.14 +0.4 4,488,365 - SO4312 0.15 +0.7 4,488,399 + SO4312 0.16 +1.1 4,488,407 - SO4312 0.17 +1.6 4,488,472 + SO4312 0.19 +1.4 4,488,472 + SO4312 0.19 +2.1 4,488,472 + SO4312 0.19 -0.4 4,488,472 + SO4312 0.19 +1.3 4,488,480 - SO4312 0.20 +1.8 4,488,480 - SO4312 0.20 +1.6 4,488,480 - SO4312 0.20 +1.8 4,488,501 - SO4312 0.20 +1.3 4,488,504 - SO4312 0.21 +1.9 4,488,570 - SO4312 0.23 +1.6 4,488,570 - SO4312 0.23 +1.3 4,488,619 - SO4312 0.25 +1.5 4,488,619 - SO4312 0.25 +0.8 4,488,685 + SO4312 0.28 +2.5 4,488,806 + SO4312 0.33 +2.3 4,488,858 + SO4312 0.35 +1.1 4,488,858 + SO4312 0.35 +0.3 4,488,858 + SO4312 0.35 +1.4 4,488,858 + SO4312 0.35 +1.1 4,488,866 - SO4312 0.36 +1.4 4,488,866 - SO4312 0.36 +1.5 4,488,866 - SO4312 0.36 +1.6 4,488,868 + SO4312 0.36 +1.9 4,488,868 + SO4312 0.36 +0.2 4,488,868 + SO4312 0.36 +2.2 4,488,868 + SO4312 0.36 +0.4 4,488,868 + SO4312 0.36 +1.7 4,488,868 + SO4312 0.36 +2.0 4,488,874 + SO4312 0.36 +1.4 4,488,876 - SO4312 0.36 +1.1 4,488,876 - +2.3 4,488,924 - SO4312 0.38 +1.6 4,488,948 + SO4312 0.39 +1.0 4,488,948 + SO4312 0.39 +0.8 4,488,958 + SO4312 0.39 +1.2 4,488,984 + SO4312 0.40 +1.1 4,489,029 - SO4312 0.42 -0.1 4,489,030 + SO4312 0.42 +1.6 4,489,075 + SO4312 0.44 +0.8 4,489,075 + SO4312 0.44 +0.2 4,489,075 + SO4312 0.44 +1.8 4,489,131 - SO4312 0.47 +0.7 4,489,195 - SO4312 0.49 +1.7 4,489,224 - SO4312 0.50 +1.1 4,489,231 - SO4312 0.51 +1.6 4,489,313 - SO4312 0.54 +0.9 4,489,317 + SO4312 0.54 +1.1 4,489,357 + SO4312 0.56 +2.0 4,489,453 - SO4312 0.60 +1.9 4,489,453 - SO4312 0.60 +1.2 4,489,467 + SO4312 0.60 +1.7 4,489,467 + SO4312 0.60 +1.7 4,489,497 - SO4312 0.62 +1.1 4,489,532 - SO4312 0.63 +0.6 4,489,663 + SO4312 0.69 +1.8 4,489,726 - SO4312 0.71 +1.2 4,489,740 + SO4312 0.72 +0.9 4,489,800 - SO4312 0.74 +0.8 4,489,805 - SO4312 0.74 +1.4 4,489,901 - SO4312 0.78 +1.7 4,490,010 - SO4312 0.83 +1.5 4,490,113 + SO4312 0.87 +0.7 4,490,113 + SO4312 0.87 +1.8 4,490,139 + SO4312 0.88 +2.4 4,490,148 + SO4312 0.89 +1.4 4,490,207 + +1.1 4,490,207 + +1.3 4,490,247 - +2.4 4,490,345 + +1.6 4,490,352 + +1.6 4,490,359 - +1.0 4,490,422 + +1.4 4,490,430 - +0.7 4,490,453 - -0.4 4,490,521 + +0.2 4,490,529 - +0.2
Or see this region's nucleotide sequence