Experiment: Gelatin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrA-2 and nqrB-2 overlap by 1 nucleotides nqrB-2 and nqrC-2 overlap by 8 nucleotides nqrC-2 and nqrD-2 overlap by 1 nucleotides
SO1103: nqrA-2 - NADH:ubiquinone oxidoreductase, Na translocating, alpha subunit (NCBI ptt file), at 1,146,236 to 1,147,570
nqrA-2
SO1104: nqrB-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrB (NCBI ptt file), at 1,147,570 to 1,148,769
nqrB-2
SO1105: nqrC-2 - NADH:ubiquinone oxidoreductase, Na translocating, gamma subunit (NCBI ptt file), at 1,148,762 to 1,149,550
nqrC-2
SO1106: nqrD-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 1,149,550 to 1,150,182
nqrD-2
Position (kb)
1147
1148
1149 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1146.706 kb on + strand, within nqrA-2 at 1146.798 kb on + strand, within nqrA-2 at 1147.050 kb on - strand, within nqrA-2 at 1147.196 kb on - strand, within nqrA-2 at 1147.366 kb on + strand, within nqrA-2 at 1147.458 kb on + strand at 1147.497 kb on - strand at 1147.532 kb on - strand at 1147.766 kb on - strand, within nqrB-2 at 1147.981 kb on + strand, within nqrB-2 at 1148.277 kb on - strand, within nqrB-2 at 1148.282 kb on + strand, within nqrB-2 at 1148.429 kb on + strand, within nqrB-2 at 1148.736 kb on + strand at 1149.336 kb on - strand, within nqrC-2 at 1149.408 kb on - strand, within nqrC-2
Per-strain Table
Position Strand Gene LocusTag Fraction Gelatin (C) remove 1,146,706 + nqrA-2 SO1103 0.35 -2.3 1,146,798 + nqrA-2 SO1103 0.42 -1.7 1,147,050 - nqrA-2 SO1103 0.61 -2.4 1,147,196 - nqrA-2 SO1103 0.72 -2.4 1,147,366 + nqrA-2 SO1103 0.85 -4.4 1,147,458 + -1.1 1,147,497 - -2.4 1,147,532 - -3.3 1,147,766 - nqrB-2 SO1104 0.16 -3.8 1,147,981 + nqrB-2 SO1104 0.34 -1.7 1,148,277 - nqrB-2 SO1104 0.59 -3.7 1,148,282 + nqrB-2 SO1104 0.59 -3.1 1,148,429 + nqrB-2 SO1104 0.72 -2.7 1,148,736 + -2.7 1,149,336 - nqrC-2 SO1105 0.73 -3.2 1,149,408 - nqrC-2 SO1105 0.82 -2.7
Or see this region's nucleotide sequence