Experiment: D-Maltose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0820 and Dshi_5001 are separated by 81 nucleotides Dshi_5001 and Dshi_0821 are separated by 13 nucleotides Dshi_0821 and Dshi_0822 are separated by 197 nucleotides Dshi_0822 and Dshi_0823 are separated by 4 nucleotides
Dshi_0820: Dshi_0820 - alpha/beta hydrolase fold (RefSeq), at 824,267 to 825,034
_0820
Dshi_5001: Dshi_5001 - hypothtical protein (RefSeq), at 825,116 to 825,505
_5001
Dshi_0821: Dshi_0821 - Glycine hydroxymethyltransferase (RefSeq), at 825,519 to 826,814
_0821
Dshi_0822: Dshi_0822 - NAD(+) kinase (RefSeq), at 827,012 to 827,779
_0822
Dshi_0823: Dshi_0823 - two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq), at 827,784 to 829,175
_0823
Position (kb) 825
826
827 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 824.686 kb on + strand, within Dshi_0820 at 824.794 kb on - strand, within Dshi_0820 at 824.876 kb on - strand, within Dshi_0820 at 824.917 kb on + strand, within Dshi_0820 at 824.969 kb on - strand at 825.020 kb on - strand at 825.064 kb on - strand at 825.121 kb on - strand at 825.125 kb on - strand at 825.298 kb on - strand, within Dshi_5001 at 825.309 kb on + strand, within Dshi_5001 at 825.379 kb on + strand, within Dshi_5001 at 825.494 kb on + strand at 825.525 kb on - strand at 825.528 kb on + strand at 825.692 kb on + strand, within Dshi_0821 at 825.713 kb on + strand, within Dshi_0821 at 825.799 kb on - strand, within Dshi_0821 at 825.842 kb on + strand, within Dshi_0821 at 825.850 kb on - strand, within Dshi_0821 at 826.342 kb on - strand, within Dshi_0821 at 826.435 kb on - strand, within Dshi_0821 at 826.610 kb on + strand, within Dshi_0821 at 826.623 kb on - strand, within Dshi_0821 at 826.643 kb on + strand, within Dshi_0821 at 826.646 kb on - strand, within Dshi_0821 at 826.846 kb on - strand at 826.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose (C) remove 824,686 + Dshi_0820 0.55 +0.4 824,794 - Dshi_0820 0.69 +0.0 824,876 - Dshi_0820 0.79 -0.0 824,917 + Dshi_0820 0.85 -1.9 824,969 - +0.0 825,020 - +0.7 825,064 - -0.4 825,121 - -0.2 825,125 - +0.1 825,298 - Dshi_5001 0.47 -1.9 825,309 + Dshi_5001 0.49 -0.0 825,379 + Dshi_5001 0.67 +1.0 825,494 + -0.4 825,525 - -0.6 825,528 + +0.9 825,692 + Dshi_0821 0.13 -1.6 825,713 + Dshi_0821 0.15 -2.9 825,799 - Dshi_0821 0.22 -1.3 825,842 + Dshi_0821 0.25 -1.9 825,850 - Dshi_0821 0.26 -1.6 826,342 - Dshi_0821 0.64 -2.6 826,435 - Dshi_0821 0.71 -2.7 826,610 + Dshi_0821 0.84 -4.5 826,623 - Dshi_0821 0.85 -2.8 826,643 + Dshi_0821 0.87 -3.5 826,646 - Dshi_0821 0.87 -3.6 826,846 - -0.0 826,847 + -0.5
Or see this region's nucleotide sequence