Experiment: D-Maltose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0192 and Dshi_0193 are separated by 77 nucleotides Dshi_0193 and Dshi_0194 are separated by 4 nucleotides Dshi_0194 and Dshi_0195 are separated by 37 nucleotides Dshi_0195 and Dshi_0196 are separated by 25 nucleotides
Dshi_0192: Dshi_0192 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (RefSeq), at 192,409 to 192,978
_0192
Dshi_0193: Dshi_0193 - DNA-directed RNA polymerase, omega subunit (RefSeq), at 193,056 to 193,412
_0193
Dshi_0194: Dshi_0194 - (p)ppGpp synthetase I, SpoT/RelA (RefSeq), at 193,417 to 195,561
_0194
Dshi_0195: Dshi_0195 - hypothetical protein (RefSeq), at 195,599 to 196,255
_0195
Dshi_0196: Dshi_0196 - pyridoxal phosphate biosynthetic protein PdxJ (RefSeq), at 196,281 to 197,045
_0196
Position (kb) 193
194
195
196 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 193.396 kb on - strand at 193.546 kb on + strand at 193.728 kb on + strand, within Dshi_0194 at 193.803 kb on + strand, within Dshi_0194 at 193.826 kb on - strand, within Dshi_0194 at 193.992 kb on - strand, within Dshi_0194 at 194.013 kb on - strand, within Dshi_0194 at 194.077 kb on + strand, within Dshi_0194 at 194.345 kb on + strand, within Dshi_0194 at 194.356 kb on + strand, within Dshi_0194 at 194.376 kb on - strand, within Dshi_0194 at 194.464 kb on - strand, within Dshi_0194 at 194.574 kb on + strand, within Dshi_0194 at 194.609 kb on + strand, within Dshi_0194 at 194.615 kb on + strand, within Dshi_0194 at 194.648 kb on - strand, within Dshi_0194 at 194.697 kb on + strand, within Dshi_0194 at 194.709 kb on + strand, within Dshi_0194 at 194.717 kb on - strand, within Dshi_0194 at 194.748 kb on + strand, within Dshi_0194 at 194.879 kb on - strand, within Dshi_0194 at 194.880 kb on - strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.010 kb on + strand, within Dshi_0194 at 195.104 kb on + strand, within Dshi_0194 at 195.200 kb on + strand, within Dshi_0194 at 195.208 kb on - strand, within Dshi_0194 at 195.219 kb on - strand, within Dshi_0194 at 195.290 kb on + strand, within Dshi_0194 at 195.316 kb on + strand, within Dshi_0194 at 195.454 kb on + strand at 195.460 kb on - strand at 195.475 kb on - strand at 195.475 kb on - strand at 195.549 kb on - strand at 195.554 kb on + strand at 195.562 kb on - strand at 195.666 kb on - strand, within Dshi_0195 at 195.666 kb on - strand, within Dshi_0195 at 195.678 kb on + strand, within Dshi_0195 at 195.887 kb on - strand, within Dshi_0195 at 195.887 kb on - strand, within Dshi_0195 at 195.904 kb on + strand, within Dshi_0195 at 195.912 kb on - strand, within Dshi_0195 at 195.957 kb on + strand, within Dshi_0195 at 195.963 kb on - strand, within Dshi_0195 at 195.972 kb on + strand, within Dshi_0195 at 195.990 kb on - strand, within Dshi_0195 at 195.995 kb on + strand, within Dshi_0195 at 196.072 kb on + strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.142 kb on - strand, within Dshi_0195 at 196.171 kb on + strand, within Dshi_0195 at 196.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose (C) remove 193,396 - -0.1 193,546 + -0.7 193,728 + Dshi_0194 0.14 -2.1 193,803 + Dshi_0194 0.18 -2.0 193,826 - Dshi_0194 0.19 -1.9 193,992 - Dshi_0194 0.27 -0.9 194,013 - Dshi_0194 0.28 -0.3 194,077 + Dshi_0194 0.31 -1.4 194,345 + Dshi_0194 0.43 -2.6 194,356 + Dshi_0194 0.44 -0.8 194,376 - Dshi_0194 0.45 -3.5 194,464 - Dshi_0194 0.49 +0.3 194,574 + Dshi_0194 0.54 -1.1 194,609 + Dshi_0194 0.56 -1.1 194,615 + Dshi_0194 0.56 -1.4 194,648 - Dshi_0194 0.57 -0.9 194,697 + Dshi_0194 0.60 -1.9 194,709 + Dshi_0194 0.60 -2.1 194,717 - Dshi_0194 0.61 -0.6 194,748 + Dshi_0194 0.62 -1.6 194,879 - Dshi_0194 0.68 -2.5 194,880 - Dshi_0194 0.68 +0.9 195,010 + Dshi_0194 0.74 -1.7 195,010 + Dshi_0194 0.74 -0.4 195,010 + Dshi_0194 0.74 -0.5 195,010 + Dshi_0194 0.74 -1.6 195,010 + Dshi_0194 0.74 -0.8 195,104 + Dshi_0194 0.79 -1.3 195,200 + Dshi_0194 0.83 +0.3 195,208 - Dshi_0194 0.83 -1.1 195,219 - Dshi_0194 0.84 -0.8 195,290 + Dshi_0194 0.87 -0.1 195,316 + Dshi_0194 0.89 -0.3 195,454 + -0.5 195,460 - -1.5 195,475 - -0.8 195,475 - -1.1 195,549 - -0.7 195,554 + +0.3 195,562 - -0.6 195,666 - Dshi_0195 0.10 -0.6 195,666 - Dshi_0195 0.10 +0.9 195,678 + Dshi_0195 0.12 -0.2 195,887 - Dshi_0195 0.44 -0.5 195,887 - Dshi_0195 0.44 +0.2 195,904 + Dshi_0195 0.46 -0.4 195,912 - Dshi_0195 0.48 -0.7 195,957 + Dshi_0195 0.54 +0.2 195,963 - Dshi_0195 0.55 +0.7 195,972 + Dshi_0195 0.57 -0.1 195,990 - Dshi_0195 0.60 -1.2 195,995 + Dshi_0195 0.60 -0.6 196,072 + Dshi_0195 0.72 -0.8 196,080 - Dshi_0195 0.73 -0.2 196,080 - Dshi_0195 0.73 +0.6 196,080 - Dshi_0195 0.73 -0.5 196,142 - Dshi_0195 0.83 -0.2 196,171 + Dshi_0195 0.87 +0.6 196,295 - -1.0
Or see this region's nucleotide sequence