Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpG and glpE overlap by 4 nucleotides glpE and tdh are separated by 98 nucleotides tdh and kbl are separated by 11 nucleotides
SO4671: glpG - glpG protein (NCBI ptt file), at 4,869,710 to 4,870,552
glpG
SO4672: glpE - glpE protein (NCBI ptt file), at 4,870,549 to 4,870,854
glpE
SO4673: tdh - threonine 3-dehydrogenase (NCBI ptt file), at 4,870,953 to 4,871,978
tdh
SO4674: kbl - 2-amino-3-ketobutyrate coenzyme A ligase (NCBI ptt file), at 4,871,990 to 4,873,183
kbl
Position (kb)
4870
4871
4872 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4869.968 kb on + strand, within glpG at 4869.986 kb on - strand, within glpG at 4869.986 kb on - strand, within glpG at 4869.998 kb on - strand, within glpG at 4870.119 kb on - strand, within glpG at 4870.191 kb on + strand, within glpG at 4870.202 kb on + strand, within glpG at 4870.218 kb on + strand, within glpG at 4870.342 kb on + strand, within glpG at 4870.342 kb on + strand, within glpG at 4870.350 kb on - strand, within glpG at 4870.488 kb on + strand at 4870.488 kb on + strand at 4870.496 kb on - strand at 4870.503 kb on + strand at 4870.508 kb on + strand at 4870.516 kb on - strand at 4870.544 kb on - strand at 4870.610 kb on - strand, within glpE at 4870.681 kb on + strand, within glpE at 4870.681 kb on + strand, within glpE at 4870.804 kb on - strand, within glpE at 4870.810 kb on + strand, within glpE at 4870.894 kb on - strand at 4870.910 kb on + strand at 4870.913 kb on - strand at 4870.916 kb on + strand at 4870.919 kb on + strand at 4870.924 kb on - strand at 4870.980 kb on + strand at 4871.039 kb on + strand at 4871.058 kb on + strand, within tdh at 4871.058 kb on + strand, within tdh at 4871.066 kb on - strand, within tdh at 4871.066 kb on - strand, within tdh at 4871.141 kb on - strand, within tdh at 4871.191 kb on - strand, within tdh at 4871.276 kb on + strand, within tdh at 4871.314 kb on + strand, within tdh at 4871.367 kb on + strand, within tdh at 4871.372 kb on + strand, within tdh at 4871.382 kb on + strand, within tdh at 4871.408 kb on - strand, within tdh at 4871.478 kb on - strand, within tdh at 4871.519 kb on - strand, within tdh at 4871.555 kb on - strand, within tdh at 4871.616 kb on + strand, within tdh at 4871.639 kb on - strand, within tdh at 4871.644 kb on + strand, within tdh at 4871.645 kb on - strand, within tdh at 4871.645 kb on - strand, within tdh at 4871.650 kb on - strand, within tdh at 4871.650 kb on - strand, within tdh at 4871.658 kb on + strand, within tdh at 4871.714 kb on - strand, within tdh at 4871.714 kb on - strand, within tdh at 4871.972 kb on - strand at 4872.034 kb on - strand at 4872.049 kb on + strand at 4872.056 kb on + strand at 4872.064 kb on - strand at 4872.083 kb on + strand at 4872.098 kb on + strand at 4872.130 kb on + strand, within kbl at 4872.336 kb on + strand, within kbl at 4872.344 kb on - strand, within kbl at 4872.370 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.443 kb on + strand, within kbl at 4872.449 kb on + strand, within kbl at 4872.451 kb on - strand, within kbl at 4872.451 kb on - strand, within kbl at 4872.451 kb on - strand, within kbl at 4872.451 kb on - strand, within kbl at 4872.451 kb on - strand, within kbl at 4872.451 kb on - strand, within kbl at 4872.451 kb on - strand, within kbl at 4872.457 kb on - strand, within kbl at 4872.462 kb on - strand, within kbl at 4872.510 kb on - strand, within kbl at 4872.543 kb on + strand, within kbl at 4872.551 kb on - strand, within kbl at 4872.559 kb on - strand, within kbl at 4872.598 kb on - strand, within kbl at 4872.598 kb on - strand, within kbl at 4872.600 kb on + strand, within kbl at 4872.608 kb on - strand, within kbl at 4872.767 kb on + strand, within kbl at 4872.778 kb on + strand, within kbl at 4872.786 kb on - strand, within kbl at 4872.787 kb on - strand, within kbl at 4872.892 kb on + strand, within kbl at 4872.903 kb on + strand, within kbl at 4872.966 kb on - strand, within kbl
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 4,869,968 + glpG SO4671 0.31 +0.7 4,869,986 - glpG SO4671 0.33 +0.8 4,869,986 - glpG SO4671 0.33 -0.5 4,869,998 - glpG SO4671 0.34 -0.0 4,870,119 - glpG SO4671 0.49 -0.8 4,870,191 + glpG SO4671 0.57 +0.1 4,870,202 + glpG SO4671 0.58 -0.5 4,870,218 + glpG SO4671 0.60 +0.6 4,870,342 + glpG SO4671 0.75 -0.1 4,870,342 + glpG SO4671 0.75 +0.2 4,870,350 - glpG SO4671 0.76 -1.0 4,870,488 + +0.7 4,870,488 + +1.1 4,870,496 - +1.2 4,870,503 + +0.3 4,870,508 + +1.2 4,870,516 - -0.4 4,870,544 - +0.1 4,870,610 - glpE SO4672 0.20 +0.5 4,870,681 + glpE SO4672 0.43 +0.6 4,870,681 + glpE SO4672 0.43 +0.7 4,870,804 - glpE SO4672 0.83 +0.2 4,870,810 + glpE SO4672 0.85 +0.5 4,870,894 - -0.7 4,870,910 + -0.1 4,870,913 - +0.3 4,870,916 + +2.9 4,870,919 + +0.6 4,870,924 - -1.2 4,870,980 + +0.1 4,871,039 + -0.7 4,871,058 + tdh SO4673 0.10 -0.7 4,871,058 + tdh SO4673 0.10 -2.4 4,871,066 - tdh SO4673 0.11 -2.5 4,871,066 - tdh SO4673 0.11 -1.9 4,871,141 - tdh SO4673 0.18 -1.2 4,871,191 - tdh SO4673 0.23 -1.5 4,871,276 + tdh SO4673 0.31 -1.4 4,871,314 + tdh SO4673 0.35 -0.2 4,871,367 + tdh SO4673 0.40 -2.2 4,871,372 + tdh SO4673 0.41 -1.6 4,871,382 + tdh SO4673 0.42 -1.5 4,871,408 - tdh SO4673 0.44 -1.4 4,871,478 - tdh SO4673 0.51 -3.6 4,871,519 - tdh SO4673 0.55 -2.9 4,871,555 - tdh SO4673 0.59 -0.5 4,871,616 + tdh SO4673 0.65 -1.2 4,871,639 - tdh SO4673 0.67 -1.1 4,871,644 + tdh SO4673 0.67 -1.5 4,871,645 - tdh SO4673 0.67 -0.8 4,871,645 - tdh SO4673 0.67 -1.7 4,871,650 - tdh SO4673 0.68 -2.1 4,871,650 - tdh SO4673 0.68 -1.8 4,871,658 + tdh SO4673 0.69 -3.1 4,871,714 - tdh SO4673 0.74 -1.2 4,871,714 - tdh SO4673 0.74 -1.2 4,871,972 - -2.4 4,872,034 - -0.1 4,872,049 + -4.8 4,872,056 + -2.1 4,872,064 - -0.1 4,872,083 + -3.0 4,872,098 + +0.8 4,872,130 + kbl SO4674 0.12 -2.2 4,872,336 + kbl SO4674 0.29 -0.2 4,872,344 - kbl SO4674 0.30 -0.7 4,872,370 + kbl SO4674 0.32 -0.3 4,872,443 + kbl SO4674 0.38 -0.6 4,872,443 + kbl SO4674 0.38 -2.6 4,872,443 + kbl SO4674 0.38 -0.1 4,872,443 + kbl SO4674 0.38 -1.1 4,872,443 + kbl SO4674 0.38 -1.7 4,872,443 + kbl SO4674 0.38 -1.0 4,872,443 + kbl SO4674 0.38 -1.1 4,872,443 + kbl SO4674 0.38 -1.6 4,872,443 + kbl SO4674 0.38 -0.5 4,872,449 + kbl SO4674 0.38 -2.0 4,872,451 - kbl SO4674 0.39 +0.2 4,872,451 - kbl SO4674 0.39 -1.7 4,872,451 - kbl SO4674 0.39 -2.8 4,872,451 - kbl SO4674 0.39 -0.3 4,872,451 - kbl SO4674 0.39 -0.2 4,872,451 - kbl SO4674 0.39 -1.4 4,872,451 - kbl SO4674 0.39 -1.1 4,872,457 - kbl SO4674 0.39 -0.5 4,872,462 - kbl SO4674 0.40 -0.7 4,872,510 - kbl SO4674 0.44 -1.9 4,872,543 + kbl SO4674 0.46 -1.7 4,872,551 - kbl SO4674 0.47 -1.5 4,872,559 - kbl SO4674 0.48 -1.9 4,872,598 - kbl SO4674 0.51 -2.1 4,872,598 - kbl SO4674 0.51 -0.4 4,872,600 + kbl SO4674 0.51 -0.8 4,872,608 - kbl SO4674 0.52 +1.1 4,872,767 + kbl SO4674 0.65 -1.5 4,872,778 + kbl SO4674 0.66 -1.1 4,872,786 - kbl SO4674 0.67 -0.5 4,872,787 - kbl SO4674 0.67 -0.8 4,872,892 + kbl SO4674 0.76 -0.7 4,872,903 + kbl SO4674 0.76 -1.3 4,872,966 - kbl SO4674 0.82 -0.4
Or see this region's nucleotide sequence