Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metL and metB are separated by 11 nucleotides metB and metJ are separated by 203 nucleotides metJ and SO4058 are separated by 96 nucleotides
SO4055: metL - aspartokinase II/homoserine dehydrogenase, methionine-sensitive (NCBI ptt file), at 4,208,836 to 4,211,229
metL
SO4056: metB - cystathionine gamma-synthase (NCBI ptt file), at 4,211,241 to 4,212,422
metB
SO4057: metJ - met repressor (NCBI ptt file), at 4,212,626 to 4,212,940
metJ
SO4058: SO4058 - conserved hypothetical protein (NCBI ptt file), at 4,213,037 to 4,213,780
SO4058
Position (kb)
4211
4212
4213 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4210.341 kb on + strand, within metL at 4210.349 kb on - strand, within metL at 4210.359 kb on - strand, within metL at 4210.373 kb on - strand, within metL at 4210.409 kb on - strand, within metL at 4210.418 kb on + strand, within metL at 4210.421 kb on - strand, within metL at 4210.426 kb on - strand, within metL at 4210.520 kb on + strand, within metL at 4210.541 kb on + strand, within metL at 4210.564 kb on + strand, within metL at 4210.573 kb on - strand, within metL at 4210.580 kb on + strand, within metL at 4210.621 kb on - strand, within metL at 4210.670 kb on - strand, within metL at 4210.706 kb on - strand, within metL at 4210.706 kb on - strand, within metL at 4210.720 kb on + strand, within metL at 4210.841 kb on + strand, within metL at 4210.851 kb on + strand, within metL at 4210.856 kb on + strand, within metL at 4210.859 kb on - strand, within metL at 4210.864 kb on - strand, within metL at 4210.864 kb on - strand, within metL at 4210.864 kb on - strand, within metL at 4210.866 kb on + strand, within metL at 4210.866 kb on - strand, within metL at 4210.874 kb on - strand, within metL at 4210.967 kb on - strand, within metL at 4210.993 kb on - strand at 4211.136 kb on + strand at 4211.144 kb on - strand at 4211.144 kb on - strand at 4211.270 kb on - strand at 4211.303 kb on + strand at 4211.377 kb on - strand, within metB at 4211.488 kb on + strand, within metB at 4211.495 kb on + strand, within metB at 4211.503 kb on - strand, within metB at 4211.591 kb on + strand, within metB at 4211.591 kb on + strand, within metB at 4211.591 kb on + strand, within metB at 4211.613 kb on + strand, within metB at 4211.621 kb on - strand, within metB at 4211.630 kb on - strand, within metB at 4211.637 kb on - strand, within metB at 4211.637 kb on - strand, within metB at 4211.723 kb on - strand, within metB at 4211.852 kb on - strand, within metB at 4211.881 kb on + strand, within metB at 4211.884 kb on - strand, within metB at 4211.912 kb on - strand, within metB at 4211.959 kb on + strand, within metB at 4211.959 kb on + strand, within metB at 4211.960 kb on + strand, within metB at 4211.960 kb on + strand, within metB at 4211.967 kb on - strand, within metB at 4211.967 kb on - strand, within metB at 4211.967 kb on - strand, within metB at 4211.968 kb on - strand, within metB at 4211.986 kb on + strand, within metB at 4211.986 kb on + strand, within metB at 4211.994 kb on - strand, within metB at 4212.186 kb on - strand, within metB at 4212.274 kb on - strand, within metB at 4212.575 kb on + strand at 4212.622 kb on + strand at 4212.663 kb on - strand, within metJ at 4212.704 kb on + strand, within metJ at 4212.768 kb on - strand, within metJ at 4212.840 kb on - strand, within metJ at 4212.840 kb on - strand, within metJ at 4213.074 kb on + strand at 4213.127 kb on - strand, within SO4058 at 4213.316 kb on - strand, within SO4058 at 4213.334 kb on + strand, within SO4058
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 4,210,341 + metL SO4055 0.63 +0.4 4,210,349 - metL SO4055 0.63 +1.5 4,210,359 - metL SO4055 0.64 +1.4 4,210,373 - metL SO4055 0.64 -0.1 4,210,409 - metL SO4055 0.66 +0.8 4,210,418 + metL SO4055 0.66 +0.4 4,210,421 - metL SO4055 0.66 +0.6 4,210,426 - metL SO4055 0.66 +0.8 4,210,520 + metL SO4055 0.70 +0.6 4,210,541 + metL SO4055 0.71 +1.1 4,210,564 + metL SO4055 0.72 +2.3 4,210,573 - metL SO4055 0.73 +0.8 4,210,580 + metL SO4055 0.73 +0.9 4,210,621 - metL SO4055 0.75 +1.0 4,210,670 - metL SO4055 0.77 +1.4 4,210,706 - metL SO4055 0.78 +1.2 4,210,706 - metL SO4055 0.78 +1.1 4,210,720 + metL SO4055 0.79 +0.9 4,210,841 + metL SO4055 0.84 +1.1 4,210,851 + metL SO4055 0.84 +0.6 4,210,856 + metL SO4055 0.84 +1.1 4,210,859 - metL SO4055 0.85 +0.2 4,210,864 - metL SO4055 0.85 +0.1 4,210,864 - metL SO4055 0.85 +0.7 4,210,864 - metL SO4055 0.85 +1.2 4,210,866 + metL SO4055 0.85 +0.5 4,210,866 - metL SO4055 0.85 +0.3 4,210,874 - metL SO4055 0.85 +2.1 4,210,967 - metL SO4055 0.89 +1.5 4,210,993 - +1.5 4,211,136 + +0.7 4,211,144 - +1.0 4,211,144 - +1.1 4,211,270 - +0.8 4,211,303 + -3.4 4,211,377 - metB SO4056 0.12 -3.6 4,211,488 + metB SO4056 0.21 -3.6 4,211,495 + metB SO4056 0.21 -4.5 4,211,503 - metB SO4056 0.22 -5.5 4,211,591 + metB SO4056 0.30 -3.4 4,211,591 + metB SO4056 0.30 -3.0 4,211,591 + metB SO4056 0.30 -1.2 4,211,613 + metB SO4056 0.31 -3.0 4,211,621 - metB SO4056 0.32 -5.3 4,211,630 - metB SO4056 0.33 -3.4 4,211,637 - metB SO4056 0.34 -4.2 4,211,637 - metB SO4056 0.34 -1.6 4,211,723 - metB SO4056 0.41 -5.2 4,211,852 - metB SO4056 0.52 -3.0 4,211,881 + metB SO4056 0.54 -3.9 4,211,884 - metB SO4056 0.54 -3.6 4,211,912 - metB SO4056 0.57 -4.0 4,211,959 + metB SO4056 0.61 -5.0 4,211,959 + metB SO4056 0.61 -1.8 4,211,960 + metB SO4056 0.61 -4.7 4,211,960 + metB SO4056 0.61 -2.9 4,211,967 - metB SO4056 0.61 -5.5 4,211,967 - metB SO4056 0.61 -4.8 4,211,967 - metB SO4056 0.61 -3.6 4,211,968 - metB SO4056 0.62 -4.3 4,211,986 + metB SO4056 0.63 -2.6 4,211,986 + metB SO4056 0.63 -4.1 4,211,994 - metB SO4056 0.64 -2.2 4,212,186 - metB SO4056 0.80 -3.6 4,212,274 - metB SO4056 0.87 -2.0 4,212,575 + +3.6 4,212,622 + +2.4 4,212,663 - metJ SO4057 0.12 -1.2 4,212,704 + metJ SO4057 0.25 +0.5 4,212,768 - metJ SO4057 0.45 -0.6 4,212,840 - metJ SO4057 0.68 +1.1 4,212,840 - metJ SO4057 0.68 -1.0 4,213,074 + -0.6 4,213,127 - SO4058 0.12 +0.3 4,213,316 - SO4058 0.38 -0.2 4,213,334 + SO4058 0.40 -0.4
Or see this region's nucleotide sequence