Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrC and thrB are separated by 72 nucleotides thrB and thrA overlap by 4 nucleotides
SO3413: thrC - threonine synthase (NCBI ptt file), at 3,552,916 to 3,554,199
thrC
SO3414: thrB - homoserine kinase (NCBI ptt file), at 3,554,272 to 3,555,210
thrB
SO3415: thrA - aspartokinase I/homoserine dehydrogenase, threonine-sensitive (NCBI ptt file), at 3,555,207 to 3,557,675
thrA
Position (kb)
3554
3555
3556 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3553.294 kb on + strand, within thrC at 3553.312 kb on + strand, within thrC at 3553.331 kb on + strand, within thrC at 3553.339 kb on - strand, within thrC at 3553.339 kb on - strand, within thrC at 3553.385 kb on - strand, within thrC at 3553.385 kb on - strand, within thrC at 3553.503 kb on + strand, within thrC at 3553.594 kb on + strand, within thrC at 3553.733 kb on + strand, within thrC at 3553.733 kb on + strand, within thrC at 3553.783 kb on - strand, within thrC at 3553.788 kb on - strand, within thrC at 3553.959 kb on - strand, within thrC at 3554.007 kb on + strand, within thrC at 3554.007 kb on + strand, within thrC at 3554.007 kb on + strand, within thrC at 3554.007 kb on + strand, within thrC at 3554.015 kb on - strand, within thrC at 3554.015 kb on - strand, within thrC at 3554.015 kb on - strand, within thrC at 3554.015 kb on - strand, within thrC at 3554.051 kb on + strand, within thrC at 3554.103 kb on - strand at 3554.122 kb on + strand at 3554.151 kb on + strand at 3554.221 kb on - strand at 3554.224 kb on + strand at 3554.232 kb on - strand at 3554.244 kb on + strand at 3554.279 kb on + strand at 3554.279 kb on + strand at 3554.279 kb on + strand at 3554.481 kb on - strand, within thrB at 3554.498 kb on + strand, within thrB at 3554.505 kb on - strand, within thrB at 3554.556 kb on + strand, within thrB at 3554.612 kb on - strand, within thrB at 3554.612 kb on - strand, within thrB at 3554.656 kb on + strand, within thrB at 3554.689 kb on - strand, within thrB at 3554.689 kb on - strand, within thrB at 3554.694 kb on + strand, within thrB at 3554.694 kb on + strand, within thrB at 3554.784 kb on + strand, within thrB at 3554.795 kb on - strand, within thrB at 3554.892 kb on + strand, within thrB at 3554.911 kb on + strand, within thrB at 3554.918 kb on - strand, within thrB at 3554.955 kb on - strand, within thrB at 3555.075 kb on + strand, within thrB at 3555.092 kb on + strand, within thrB at 3555.206 kb on + strand at 3555.206 kb on + strand at 3555.218 kb on + strand at 3555.224 kb on - strand at 3555.280 kb on - strand at 3555.295 kb on - strand at 3555.305 kb on - strand at 3555.310 kb on - strand at 3555.398 kb on - strand at 3555.439 kb on - strand at 3555.448 kb on + strand at 3555.448 kb on + strand at 3555.456 kb on - strand, within thrA at 3555.456 kb on - strand, within thrA at 3555.638 kb on + strand, within thrA at 3555.726 kb on - strand, within thrA at 3555.780 kb on - strand, within thrA at 3555.878 kb on + strand, within thrA at 3556.011 kb on - strand, within thrA at 3556.027 kb on + strand, within thrA at 3556.058 kb on + strand, within thrA
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 3,553,294 + thrC SO3413 0.29 -3.9 3,553,312 + thrC SO3413 0.31 -5.5 3,553,331 + thrC SO3413 0.32 -3.5 3,553,339 - thrC SO3413 0.33 -3.7 3,553,339 - thrC SO3413 0.33 -2.7 3,553,385 - thrC SO3413 0.37 +0.4 3,553,385 - thrC SO3413 0.37 -1.9 3,553,503 + thrC SO3413 0.46 -2.7 3,553,594 + thrC SO3413 0.53 -4.8 3,553,733 + thrC SO3413 0.64 -3.5 3,553,733 + thrC SO3413 0.64 -2.2 3,553,783 - thrC SO3413 0.68 -6.2 3,553,788 - thrC SO3413 0.68 -2.9 3,553,959 - thrC SO3413 0.81 -2.6 3,554,007 + thrC SO3413 0.85 -4.6 3,554,007 + thrC SO3413 0.85 -3.8 3,554,007 + thrC SO3413 0.85 -1.2 3,554,007 + thrC SO3413 0.85 -3.7 3,554,015 - thrC SO3413 0.86 -5.3 3,554,015 - thrC SO3413 0.86 -3.9 3,554,015 - thrC SO3413 0.86 -3.3 3,554,015 - thrC SO3413 0.86 -4.0 3,554,051 + thrC SO3413 0.88 -3.5 3,554,103 - -3.7 3,554,122 + -3.3 3,554,151 + -5.9 3,554,221 - -1.0 3,554,224 + -5.4 3,554,232 - -1.0 3,554,244 + -2.4 3,554,279 + -6.6 3,554,279 + -4.0 3,554,279 + -4.6 3,554,481 - thrB SO3414 0.22 -2.4 3,554,498 + thrB SO3414 0.24 -3.6 3,554,505 - thrB SO3414 0.25 -3.0 3,554,556 + thrB SO3414 0.30 -1.2 3,554,612 - thrB SO3414 0.36 -1.9 3,554,612 - thrB SO3414 0.36 -4.6 3,554,656 + thrB SO3414 0.41 -4.9 3,554,689 - thrB SO3414 0.44 -3.3 3,554,689 - thrB SO3414 0.44 -4.4 3,554,694 + thrB SO3414 0.45 -3.5 3,554,694 + thrB SO3414 0.45 -5.4 3,554,784 + thrB SO3414 0.55 -1.6 3,554,795 - thrB SO3414 0.56 -4.4 3,554,892 + thrB SO3414 0.66 -3.8 3,554,911 + thrB SO3414 0.68 -4.5 3,554,918 - thrB SO3414 0.69 -2.9 3,554,955 - thrB SO3414 0.73 -4.1 3,555,075 + thrB SO3414 0.86 -2.9 3,555,092 + thrB SO3414 0.87 -4.7 3,555,206 + -3.7 3,555,206 + -5.2 3,555,218 + -3.0 3,555,224 - -0.1 3,555,280 - -1.5 3,555,295 - -2.8 3,555,305 - -2.4 3,555,310 - -2.0 3,555,398 - -3.2 3,555,439 - -3.6 3,555,448 + -2.6 3,555,448 + -3.9 3,555,456 - thrA SO3415 0.10 -3.7 3,555,456 - thrA SO3415 0.10 -0.9 3,555,638 + thrA SO3415 0.17 -5.3 3,555,726 - thrA SO3415 0.21 -2.8 3,555,780 - thrA SO3415 0.23 -2.7 3,555,878 + thrA SO3415 0.27 -3.7 3,556,011 - thrA SO3415 0.33 -2.6 3,556,027 + thrA SO3415 0.33 -4.3 3,556,058 + thrA SO3415 0.34 -2.4
Or see this region's nucleotide sequence