Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaA and guaB are separated by 116 nucleotides guaB and xseA are separated by 122 nucleotides
SO3292: guaA - GMP synthase (NCBI ptt file), at 3,435,675 to 3,437,252
guaA
SO3293: guaB - inosine-5'-monophosphate dehydrogenase (NCBI ptt file), at 3,437,369 to 3,438,835
guaB
SO3294: xseA - exodeoxyribonuclease VII, large subunit (NCBI ptt file), at 3,438,958 to 3,440,295
xseA
Position (kb)
3437
3438
3439 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3436.624 kb on + strand, within guaA at 3436.711 kb on - strand, within guaA at 3436.839 kb on + strand, within guaA at 3436.882 kb on + strand, within guaA at 3436.882 kb on + strand, within guaA at 3436.882 kb on - strand, within guaA at 3436.890 kb on - strand, within guaA at 3436.900 kb on - strand, within guaA at 3436.973 kb on + strand, within guaA at 3436.973 kb on + strand, within guaA at 3437.098 kb on - strand at 3437.840 kb on + strand, within guaB at 3437.840 kb on + strand, within guaB at 3437.932 kb on - strand, within guaB at 3437.935 kb on + strand, within guaB at 3437.967 kb on + strand at 3438.044 kb on + strand, within guaB at 3438.117 kb on + strand at 3438.117 kb on + strand, within guaB at 3438.125 kb on - strand, within guaB at 3438.148 kb on - strand, within guaB at 3438.188 kb on + strand, within guaB at 3438.236 kb on + strand at 3438.244 kb on - strand, within guaB at 3438.248 kb on + strand, within guaB at 3438.256 kb on - strand, within guaB at 3438.429 kb on - strand, within guaB at 3438.532 kb on - strand, within guaB at 3438.731 kb on + strand at 3438.766 kb on - strand at 3438.766 kb on - strand at 3438.887 kb on + strand at 3438.887 kb on + strand at 3438.887 kb on + strand at 3438.895 kb on - strand at 3438.895 kb on - strand at 3438.895 kb on - strand at 3438.996 kb on + strand at 3439.082 kb on + strand at 3439.167 kb on + strand, within xseA at 3439.277 kb on + strand, within xseA at 3439.283 kb on - strand, within xseA at 3439.285 kb on - strand, within xseA at 3439.392 kb on - strand, within xseA at 3439.403 kb on - strand, within xseA at 3439.429 kb on - strand, within xseA at 3439.438 kb on - strand, within xseA at 3439.566 kb on - strand, within xseA at 3439.671 kb on - strand, within xseA at 3439.779 kb on - strand, within xseA at 3439.779 kb on - strand, within xseA at 3439.791 kb on - strand, within xseA at 3439.801 kb on - strand, within xseA at 3439.830 kb on + strand, within xseA at 3439.830 kb on + strand, within xseA
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 3,436,624 + guaA SO3292 0.60 -1.8 3,436,711 - guaA SO3292 0.66 -1.8 3,436,839 + guaA SO3292 0.74 -2.6 3,436,882 + guaA SO3292 0.76 -1.8 3,436,882 + guaA SO3292 0.76 -0.8 3,436,882 - guaA SO3292 0.76 -2.3 3,436,890 - guaA SO3292 0.77 +0.1 3,436,900 - guaA SO3292 0.78 -3.6 3,436,973 + guaA SO3292 0.82 -2.8 3,436,973 + guaA SO3292 0.82 -2.8 3,437,098 - -1.8 3,437,840 + guaB SO3293 0.32 -2.6 3,437,840 + guaB SO3293 0.32 -0.8 3,437,932 - guaB SO3293 0.38 -1.3 3,437,935 + guaB SO3293 0.39 -0.2 3,437,967 + +0.2 3,438,044 + guaB SO3293 0.46 -3.1 3,438,117 + +0.2 3,438,117 + guaB SO3293 0.51 -2.3 3,438,125 - guaB SO3293 0.52 -3.1 3,438,148 - guaB SO3293 0.53 -3.2 3,438,188 + guaB SO3293 0.56 -3.3 3,438,236 + +0.2 3,438,244 - guaB SO3293 0.60 -1.3 3,438,248 + guaB SO3293 0.60 -2.3 3,438,256 - guaB SO3293 0.60 -3.3 3,438,429 - guaB SO3293 0.72 -1.8 3,438,532 - guaB SO3293 0.79 -1.3 3,438,731 + -1.9 3,438,766 - -1.3 3,438,766 - -2.1 3,438,887 + -0.9 3,438,887 + -0.5 3,438,887 + +1.5 3,438,895 - +0.8 3,438,895 - -0.4 3,438,895 - +0.3 3,438,996 + -0.2 3,439,082 + -0.5 3,439,167 + xseA SO3294 0.16 -0.4 3,439,277 + xseA SO3294 0.24 -0.4 3,439,283 - xseA SO3294 0.24 +0.0 3,439,285 - xseA SO3294 0.24 -1.2 3,439,392 - xseA SO3294 0.32 -1.2 3,439,403 - xseA SO3294 0.33 -0.1 3,439,429 - xseA SO3294 0.35 +0.4 3,439,438 - xseA SO3294 0.36 +0.1 3,439,566 - xseA SO3294 0.45 -1.4 3,439,671 - xseA SO3294 0.53 -1.4 3,439,779 - xseA SO3294 0.61 +0.1 3,439,779 - xseA SO3294 0.61 -0.1 3,439,791 - xseA SO3294 0.62 -0.5 3,439,801 - xseA SO3294 0.63 -0.8 3,439,830 + xseA SO3294 0.65 +0.7 3,439,830 + xseA SO3294 0.65 -0.9
Or see this region's nucleotide sequence