Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheY-3 and fliA are separated by 47 nucleotides fliA and flhG overlap by 8 nucleotides flhG and flhF are separated by 34 nucleotides
SO3209: cheY-3 - chemotaxis protein CheY (NCBI ptt file), at 3,349,724 to 3,350,107
cheY-3
SO3210: fliA - RNA polymerase sigma-27 factor (NCBI ptt file), at 3,350,155 to 3,350,868
fliA
SO3211: flhG - flagellar biosynthetic protein FlhG (NCBI ptt file), at 3,350,861 to 3,351,712
flhG
SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123
flhF
Position (kb)
3350
3351
3352 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3349.899 kb on + strand, within cheY-3 at 3350.007 kb on + strand, within cheY-3 at 3350.116 kb on - strand at 3350.191 kb on - strand at 3350.534 kb on - strand, within fliA at 3350.671 kb on - strand, within fliA at 3350.686 kb on - strand, within fliA at 3350.731 kb on - strand, within fliA at 3350.732 kb on + strand, within fliA at 3350.845 kb on + strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3351.311 kb on - strand, within flhG at 3351.457 kb on - strand, within flhG at 3351.511 kb on - strand, within flhG at 3351.571 kb on - strand, within flhG at 3351.591 kb on + strand, within flhG at 3351.638 kb on + strand at 3351.646 kb on - strand at 3351.770 kb on - strand at 3351.770 kb on - strand at 3351.982 kb on - strand, within flhF at 3352.025 kb on - strand, within flhF at 3352.032 kb on + strand, within flhF at 3352.045 kb on - strand, within flhF at 3352.109 kb on + strand, within flhF at 3352.260 kb on + strand, within flhF at 3352.310 kb on - strand, within flhF at 3352.383 kb on + strand, within flhF at 3352.621 kb on - strand, within flhF at 3352.690 kb on + strand, within flhF
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 3,349,899 + cheY-3 SO3209 0.46 +0.8 3,350,007 + cheY-3 SO3209 0.74 +0.3 3,350,116 - +0.1 3,350,191 - +2.7 3,350,534 - fliA SO3210 0.53 +2.6 3,350,671 - fliA SO3210 0.72 +2.7 3,350,686 - fliA SO3210 0.74 +2.9 3,350,731 - fliA SO3210 0.81 +3.6 3,350,732 + fliA SO3210 0.81 +4.5 3,350,845 + +4.5 3,350,853 - +3.9 3,350,853 - +3.0 3,350,853 - +1.4 3,351,311 - flhG SO3211 0.53 -4.7 3,351,457 - flhG SO3211 0.70 -4.7 3,351,511 - flhG SO3211 0.76 -1.2 3,351,571 - flhG SO3211 0.83 -4.0 3,351,591 + flhG SO3211 0.86 -0.9 3,351,638 + -1.9 3,351,646 - -3.9 3,351,770 - +0.2 3,351,770 - -0.1 3,351,982 - flhF SO3212 0.17 -1.9 3,352,025 - flhF SO3212 0.20 +0.3 3,352,032 + flhF SO3212 0.21 -1.2 3,352,045 - flhF SO3212 0.22 +0.2 3,352,109 + flhF SO3212 0.26 -1.4 3,352,260 + flhF SO3212 0.37 -1.7 3,352,310 - flhF SO3212 0.41 -0.4 3,352,383 + flhF SO3212 0.46 -0.9 3,352,621 - flhF SO3212 0.63 -0.7 3,352,690 + flhF SO3212 0.68 +0.1
Or see this region's nucleotide sequence