Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Val-3 and tRNA-Val-2 are separated by 45 nucleotides tRNA-Val-2 and tRNA-Val-1 are separated by 26 nucleotides tRNA-Val-1 and SO3132 are separated by 151 nucleotides SO3132 and dctP are separated by 393 nucleotides dctP and SO3135 are separated by 63 nucleotides SO3135 and dctM overlap by 1 nucleotides
SO_t075: tRNA-Val-3 - tRNA-Val (RefSeq), at 3,261,945 to 3,262,020
tRNA-Val-3
SO_t076: tRNA-Val-2 - tRNA-Val (RefSeq), at 3,262,066 to 3,262,141
tRNA-Val-2
SO_t077: tRNA-Val-1 - tRNA-Val (RefSeq), at 3,262,168 to 3,262,243
tRNA-Val-1
SO3132: SO3132 - hypothetical protein (NCBI ptt file), at 3,262,395 to 3,262,625
SO3132
SO3134: dctP - C4-dicarboxylate-binding periplasmic protein (NCBI ptt file), at 3,263,019 to 3,264,038
dctP
SO3135: SO3135 - C4-dicarboxylate transporter, putative (NCBI ptt file), at 3,264,102 to 3,264,761
SO3135
SO3136: dctM - C4-dicarboxylate transport protein (NCBI ptt file), at 3,264,761 to 3,266,158
dctM
Position (kb)
3263
3264
3265 Strain fitness (log2 ratio)
-2
-1
0
1 at 3262.143 kb on + strand at 3262.360 kb on + strand at 3262.817 kb on - strand at 3262.924 kb on + strand at 3262.941 kb on - strand at 3263.112 kb on - strand at 3263.283 kb on + strand, within dctP at 3263.631 kb on + strand, within dctP at 3263.641 kb on - strand, within dctP at 3263.641 kb on - strand, within dctP at 3263.720 kb on - strand, within dctP at 3263.821 kb on - strand, within dctP at 3263.823 kb on + strand, within dctP at 3263.870 kb on + strand, within dctP at 3263.870 kb on + strand, within dctP at 3263.968 kb on - strand at 3264.078 kb on + strand at 3264.176 kb on - strand, within SO3135 at 3264.238 kb on + strand, within SO3135 at 3264.238 kb on + strand, within SO3135 at 3264.238 kb on + strand, within SO3135 at 3264.238 kb on + strand, within SO3135 at 3264.244 kb on - strand, within SO3135 at 3264.246 kb on - strand, within SO3135 at 3264.246 kb on - strand, within SO3135 at 3264.246 kb on - strand, within SO3135 at 3264.246 kb on - strand, within SO3135 at 3264.246 kb on - strand, within SO3135 at 3264.246 kb on - strand, within SO3135 at 3264.246 kb on - strand, within SO3135 at 3264.275 kb on + strand, within SO3135 at 3264.283 kb on + strand, within SO3135 at 3264.283 kb on + strand, within SO3135 at 3264.283 kb on + strand, within SO3135 at 3264.283 kb on - strand, within SO3135 at 3264.283 kb on - strand, within SO3135 at 3264.283 kb on - strand, within SO3135 at 3264.283 kb on - strand, within SO3135 at 3264.291 kb on - strand, within SO3135 at 3264.291 kb on - strand, within SO3135 at 3264.291 kb on - strand, within SO3135 at 3264.291 kb on - strand, within SO3135 at 3264.291 kb on - strand, within SO3135 at 3264.309 kb on - strand, within SO3135 at 3264.377 kb on - strand, within SO3135 at 3264.415 kb on + strand, within SO3135 at 3264.447 kb on - strand, within SO3135 at 3264.511 kb on + strand, within SO3135 at 3264.514 kb on - strand, within SO3135 at 3264.546 kb on + strand, within SO3135 at 3264.554 kb on - strand, within SO3135 at 3264.648 kb on + strand, within SO3135 at 3264.700 kb on + strand at 3264.700 kb on + strand at 3264.718 kb on - strand at 3264.725 kb on + strand at 3264.741 kb on + strand at 3264.749 kb on - strand at 3264.766 kb on - strand at 3264.817 kb on - strand at 3264.835 kb on - strand at 3264.882 kb on + strand at 3264.920 kb on - strand, within dctM at 3265.012 kb on + strand, within dctM at 3265.037 kb on + strand, within dctM
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 3,262,143 + -2.2 3,262,360 + -0.9 3,262,817 - -0.9 3,262,924 + +0.3 3,262,941 - +0.8 3,263,112 - +0.5 3,263,283 + dctP SO3134 0.26 -0.2 3,263,631 + dctP SO3134 0.60 -0.8 3,263,641 - dctP SO3134 0.61 -1.4 3,263,641 - dctP SO3134 0.61 -0.2 3,263,720 - dctP SO3134 0.69 -0.3 3,263,821 - dctP SO3134 0.79 -1.2 3,263,823 + dctP SO3134 0.79 +0.6 3,263,870 + dctP SO3134 0.83 +0.2 3,263,870 + dctP SO3134 0.83 -0.7 3,263,968 - +0.3 3,264,078 + -0.7 3,264,176 - SO3135 0.11 -1.4 3,264,238 + SO3135 0.21 -0.5 3,264,238 + SO3135 0.21 -0.4 3,264,238 + SO3135 0.21 -0.8 3,264,238 + SO3135 0.21 -1.0 3,264,244 - SO3135 0.22 -0.9 3,264,246 - SO3135 0.22 -0.0 3,264,246 - SO3135 0.22 -0.9 3,264,246 - SO3135 0.22 +0.6 3,264,246 - SO3135 0.22 -0.7 3,264,246 - SO3135 0.22 +0.9 3,264,246 - SO3135 0.22 -0.4 3,264,246 - SO3135 0.22 +0.0 3,264,275 + SO3135 0.26 +0.4 3,264,283 + SO3135 0.27 +0.0 3,264,283 + SO3135 0.27 -1.3 3,264,283 + SO3135 0.27 -0.3 3,264,283 - SO3135 0.27 -1.2 3,264,283 - SO3135 0.27 -0.7 3,264,283 - SO3135 0.27 -0.0 3,264,283 - SO3135 0.27 +0.6 3,264,291 - SO3135 0.29 -0.7 3,264,291 - SO3135 0.29 -0.8 3,264,291 - SO3135 0.29 -0.2 3,264,291 - SO3135 0.29 -0.3 3,264,291 - SO3135 0.29 +0.2 3,264,309 - SO3135 0.31 -0.7 3,264,377 - SO3135 0.42 -0.0 3,264,415 + SO3135 0.47 -0.0 3,264,447 - SO3135 0.52 +0.5 3,264,511 + SO3135 0.62 -1.3 3,264,514 - SO3135 0.62 -0.8 3,264,546 + SO3135 0.67 -0.1 3,264,554 - SO3135 0.68 -0.4 3,264,648 + SO3135 0.83 +0.0 3,264,700 + +1.4 3,264,700 + -1.0 3,264,718 - -0.0 3,264,725 + -0.5 3,264,741 + -0.3 3,264,749 - +1.1 3,264,766 - -0.3 3,264,817 - -0.1 3,264,835 - -0.7 3,264,882 + +0.0 3,264,920 - dctM SO3136 0.11 -0.8 3,265,012 + dctM SO3136 0.18 -1.1 3,265,037 + dctM SO3136 0.20 -0.5
Or see this region's nucleotide sequence