Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpC/F are separated by 92 nucleotides trpC/F and trpB are separated by 119 nucleotides
SO3021: trpD - anthranilate phosphoribosyltransferase (NCBI ptt file), at 3,132,201 to 3,133,244
trpD
SO3022: trpC/F - indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (NCBI ptt file), at 3,133,337 to 3,134,746
trpC/F
SO3023: trpB - tryptophan synthase, beta subunit (NCBI ptt file), at 3,134,866 to 3,136,056
trpB
Position (kb)
3133
3134
3135 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3132.412 kb on - strand, within trpD at 3132.418 kb on - strand, within trpD at 3132.419 kb on - strand, within trpD at 3132.504 kb on + strand, within trpD at 3132.596 kb on - strand, within trpD at 3132.625 kb on + strand, within trpD at 3132.625 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.654 kb on - strand, within trpD at 3132.654 kb on - strand, within trpD at 3132.681 kb on + strand, within trpD at 3132.711 kb on + strand, within trpD at 3132.775 kb on - strand, within trpD at 3132.801 kb on + strand, within trpD at 3132.809 kb on - strand, within trpD at 3132.811 kb on + strand, within trpD at 3132.843 kb on + strand, within trpD at 3132.873 kb on + strand, within trpD at 3132.932 kb on + strand, within trpD at 3133.085 kb on - strand, within trpD at 3133.174 kb on - strand at 3133.229 kb on - strand at 3133.230 kb on - strand at 3133.268 kb on + strand at 3133.337 kb on + strand at 3133.337 kb on + strand at 3133.345 kb on - strand at 3133.406 kb on + strand at 3133.407 kb on + strand at 3133.415 kb on - strand at 3133.540 kb on - strand, within trpC/F at 3133.540 kb on - strand, within trpC/F at 3133.659 kb on + strand, within trpC/F at 3133.766 kb on + strand, within trpC/F at 3133.945 kb on + strand, within trpC/F at 3134.058 kb on + strand, within trpC/F at 3134.139 kb on + strand, within trpC/F at 3134.249 kb on + strand, within trpC/F at 3134.249 kb on + strand, within trpC/F at 3134.257 kb on - strand, within trpC/F at 3134.345 kb on + strand, within trpC/F at 3134.365 kb on - strand, within trpC/F at 3134.365 kb on - strand, within trpC/F at 3134.399 kb on - strand, within trpC/F at 3134.402 kb on + strand, within trpC/F at 3134.431 kb on - strand, within trpC/F at 3134.454 kb on + strand, within trpC/F at 3134.454 kb on + strand, within trpC/F at 3134.496 kb on - strand, within trpC/F at 3134.552 kb on - strand, within trpC/F at 3134.703 kb on + strand at 3134.797 kb on + strand at 3134.850 kb on + strand at 3134.880 kb on - strand at 3134.996 kb on - strand, within trpB at 3134.996 kb on - strand, within trpB at 3135.032 kb on + strand, within trpB at 3135.111 kb on - strand, within trpB at 3135.139 kb on + strand, within trpB at 3135.139 kb on + strand, within trpB at 3135.205 kb on + strand, within trpB at 3135.205 kb on + strand, within trpB at 3135.213 kb on - strand, within trpB at 3135.226 kb on + strand, within trpB at 3135.226 kb on + strand, within trpB at 3135.234 kb on - strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.264 kb on + strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.272 kb on - strand, within trpB at 3135.272 kb on - strand, within trpB at 3135.398 kb on + strand, within trpB at 3135.624 kb on + strand, within trpB at 3135.651 kb on - strand, within trpB at 3135.659 kb on + strand, within trpB
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 3,132,412 - trpD SO3021 0.20 -2.5 3,132,418 - trpD SO3021 0.21 -3.3 3,132,419 - trpD SO3021 0.21 -2.1 3,132,504 + trpD SO3021 0.29 -4.4 3,132,596 - trpD SO3021 0.38 -3.3 3,132,625 + trpD SO3021 0.41 -3.5 3,132,625 + trpD SO3021 0.41 -2.5 3,132,646 + trpD SO3021 0.43 -2.5 3,132,646 + trpD SO3021 0.43 -3.5 3,132,646 + trpD SO3021 0.43 -1.3 3,132,654 - trpD SO3021 0.43 -1.1 3,132,654 - trpD SO3021 0.43 -6.5 3,132,681 + trpD SO3021 0.46 -2.5 3,132,711 + trpD SO3021 0.49 -2.5 3,132,775 - trpD SO3021 0.55 +0.3 3,132,801 + trpD SO3021 0.57 -2.8 3,132,809 - trpD SO3021 0.58 -2.7 3,132,811 + trpD SO3021 0.58 -4.4 3,132,843 + trpD SO3021 0.61 -4.1 3,132,873 + trpD SO3021 0.64 -2.0 3,132,932 + trpD SO3021 0.70 -3.3 3,133,085 - trpD SO3021 0.85 -2.9 3,133,174 - -3.4 3,133,229 - -2.8 3,133,230 - -3.9 3,133,268 + -1.0 3,133,337 + -5.6 3,133,337 + -3.3 3,133,345 - -2.5 3,133,406 + -2.9 3,133,407 + -3.4 3,133,415 - +0.3 3,133,540 - trpC/F SO3022 0.14 -5.1 3,133,540 - trpC/F SO3022 0.14 -3.1 3,133,659 + trpC/F SO3022 0.23 -1.4 3,133,766 + trpC/F SO3022 0.30 -3.8 3,133,945 + trpC/F SO3022 0.43 -1.7 3,134,058 + trpC/F SO3022 0.51 -2.4 3,134,139 + trpC/F SO3022 0.57 -1.3 3,134,249 + trpC/F SO3022 0.65 -1.6 3,134,249 + trpC/F SO3022 0.65 -2.3 3,134,257 - trpC/F SO3022 0.65 -3.4 3,134,345 + trpC/F SO3022 0.71 -4.5 3,134,365 - trpC/F SO3022 0.73 -3.3 3,134,365 - trpC/F SO3022 0.73 -4.8 3,134,399 - trpC/F SO3022 0.75 -4.3 3,134,402 + trpC/F SO3022 0.76 -3.4 3,134,431 - trpC/F SO3022 0.78 -1.8 3,134,454 + trpC/F SO3022 0.79 -3.8 3,134,454 + trpC/F SO3022 0.79 -3.3 3,134,496 - trpC/F SO3022 0.82 -4.5 3,134,552 - trpC/F SO3022 0.86 -2.0 3,134,703 + -1.3 3,134,797 + -0.7 3,134,850 + -0.0 3,134,880 - -4.4 3,134,996 - trpB SO3023 0.11 -3.7 3,134,996 - trpB SO3023 0.11 -2.6 3,135,032 + trpB SO3023 0.14 -3.4 3,135,111 - trpB SO3023 0.21 -3.0 3,135,139 + trpB SO3023 0.23 -3.1 3,135,139 + trpB SO3023 0.23 -2.5 3,135,205 + trpB SO3023 0.28 -4.5 3,135,205 + trpB SO3023 0.28 -2.7 3,135,213 - trpB SO3023 0.29 -3.6 3,135,226 + trpB SO3023 0.30 -4.3 3,135,226 + trpB SO3023 0.30 -1.0 3,135,234 - trpB SO3023 0.31 -0.1 3,135,262 + trpB SO3023 0.33 -3.4 3,135,262 + trpB SO3023 0.33 -1.7 3,135,262 + trpB SO3023 0.33 -5.3 3,135,264 + trpB SO3023 0.33 -2.7 3,135,270 - trpB SO3023 0.34 -3.7 3,135,270 - trpB SO3023 0.34 -2.5 3,135,270 - trpB SO3023 0.34 -4.0 3,135,270 - trpB SO3023 0.34 -2.8 3,135,272 - trpB SO3023 0.34 -3.8 3,135,272 - trpB SO3023 0.34 -4.1 3,135,398 + trpB SO3023 0.45 -4.1 3,135,624 + trpB SO3023 0.64 -2.5 3,135,651 - trpB SO3023 0.66 -5.6 3,135,659 + trpB SO3023 0.67 -4.5
Or see this region's nucleotide sequence