Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2396 and SO2397 are separated by 45 nucleotides SO2397 and pyrF are separated by 65 nucleotides pyrF and SO2399 are separated by 42 nucleotides
SO2396: SO2396 - conserved hypothetical protein (NCBI ptt file), at 2,500,951 to 2,501,493
SO2396
SO2397: SO2397 - oxidoreductase, short-chain dehydrogenase/reductase family (NCBI ptt file), at 2,501,539 to 2,502,366
SO2397
SO2398: pyrF - orotidine 5`-phosphate decarboxylase (NCBI ptt file), at 2,502,432 to 2,503,127
pyrF
SO2399: SO2399 - conserved hypothetical protein (NCBI ptt file), at 2,503,170 to 2,504,330
SO2399
Position (kb)
2502
2503
2504 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2501.750 kb on - strand, within SO2397 at 2501.922 kb on + strand, within SO2397 at 2501.959 kb on + strand, within SO2397 at 2502.164 kb on - strand, within SO2397 at 2502.164 kb on - strand, within SO2397 at 2502.252 kb on - strand, within SO2397 at 2502.358 kb on + strand at 2502.364 kb on - strand at 2502.555 kb on + strand, within pyrF at 2502.710 kb on - strand, within pyrF at 2502.776 kb on - strand, within pyrF at 2502.804 kb on - strand, within pyrF at 2503.016 kb on - strand, within pyrF
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 2,501,750 - SO2397 0.25 -0.6 2,501,922 + SO2397 0.46 +0.6 2,501,959 + SO2397 0.51 +0.0 2,502,164 - SO2397 0.75 -0.3 2,502,164 - SO2397 0.75 -0.4 2,502,252 - SO2397 0.86 +1.5 2,502,358 + -0.6 2,502,364 - +0.5 2,502,555 + pyrF SO2398 0.18 +0.8 2,502,710 - pyrF SO2398 0.40 -1.2 2,502,776 - pyrF SO2398 0.49 -2.2 2,502,804 - pyrF SO2398 0.53 -4.1 2,503,016 - pyrF SO2398 0.84 -3.7
Or see this region's nucleotide sequence