Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mvaB and SO1894 are separated by 1 nucleotides SO1894 and SO1895 overlap by 1 nucleotides SO1895 and pccB-1 are separated by 46 nucleotides
SO1893: mvaB - hydroxymethylglutaryl-CoA lyase (NCBI ptt file), at 1,989,479 to 1,990,426
mvaB
SO1894: SO1894 - acetyl-CoA carboxylase, biotin carboxylase, putative (NCBI ptt file), at 1,990,428 to 1,992,512
SO1894
SO1895: SO1895 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,992,512 to 1,993,378
SO1895
SO1896: pccB-1 - 3-methylcrotonyl CoA carboxylase, beta subunit (NCBI ptt file), at 1,993,425 to 1,995,032
pccB-1
Position (kb)
1990
1991
1992
1993 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1990.469 kb on + strand at 1990.553 kb on - strand at 1990.591 kb on + strand at 1990.591 kb on + strand at 1990.591 kb on + strand at 1990.599 kb on - strand at 1990.599 kb on - strand at 1990.599 kb on - strand at 1990.623 kb on + strand at 1990.631 kb on - strand at 1990.633 kb on + strand at 1990.700 kb on - strand, within SO1894 at 1990.726 kb on + strand, within SO1894 at 1990.726 kb on + strand, within SO1894 at 1990.734 kb on - strand, within SO1894 at 1990.817 kb on + strand, within SO1894 at 1990.959 kb on - strand, within SO1894 at 1990.964 kb on - strand, within SO1894 at 1990.994 kb on - strand, within SO1894 at 1991.175 kb on - strand, within SO1894 at 1991.262 kb on + strand, within SO1894 at 1991.386 kb on + strand, within SO1894 at 1991.474 kb on + strand, within SO1894 at 1991.482 kb on - strand, within SO1894 at 1991.592 kb on + strand, within SO1894 at 1991.611 kb on + strand, within SO1894 at 1991.789 kb on + strand, within SO1894 at 1992.007 kb on + strand, within SO1894 at 1992.007 kb on + strand, within SO1894 at 1992.015 kb on - strand, within SO1894 at 1992.194 kb on + strand, within SO1894 at 1992.234 kb on + strand, within SO1894 at 1992.409 kb on + strand at 1992.409 kb on + strand at 1992.409 kb on + strand at 1992.409 kb on + strand at 1992.409 kb on + strand at 1992.417 kb on - strand at 1992.417 kb on - strand at 1992.417 kb on - strand at 1992.427 kb on - strand at 1992.495 kb on + strand at 1992.584 kb on + strand at 1992.663 kb on + strand, within SO1895 at 1992.826 kb on + strand, within SO1895 at 1992.968 kb on + strand, within SO1895 at 1992.980 kb on + strand, within SO1895 at 1992.980 kb on + strand, within SO1895 at 1993.056 kb on + strand, within SO1895 at 1993.228 kb on + strand, within SO1895 at 1993.261 kb on + strand, within SO1895 at 1993.312 kb on + strand at 1993.355 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 1,990,469 + +0.9 1,990,553 - -1.1 1,990,591 + -0.4 1,990,591 + -0.5 1,990,591 + -3.8 1,990,599 - -0.9 1,990,599 - -3.4 1,990,599 - -1.5 1,990,623 + -2.2 1,990,631 - -2.7 1,990,633 + -3.8 1,990,700 - SO1894 0.13 -1.9 1,990,726 + SO1894 0.14 -2.8 1,990,726 + SO1894 0.14 -2.3 1,990,734 - SO1894 0.15 -2.9 1,990,817 + SO1894 0.19 -1.5 1,990,959 - SO1894 0.25 -1.9 1,990,964 - SO1894 0.26 -3.1 1,990,994 - SO1894 0.27 -1.1 1,991,175 - SO1894 0.36 -2.4 1,991,262 + SO1894 0.40 -2.2 1,991,386 + SO1894 0.46 -3.4 1,991,474 + SO1894 0.50 -2.7 1,991,482 - SO1894 0.51 -1.8 1,991,592 + SO1894 0.56 -3.2 1,991,611 + SO1894 0.57 +0.8 1,991,789 + SO1894 0.65 -2.5 1,992,007 + SO1894 0.76 -4.4 1,992,007 + SO1894 0.76 -1.0 1,992,015 - SO1894 0.76 -2.8 1,992,194 + SO1894 0.85 -3.1 1,992,234 + SO1894 0.87 -0.3 1,992,409 + +0.8 1,992,409 + -2.9 1,992,409 + -1.9 1,992,409 + -0.9 1,992,409 + -3.4 1,992,417 - -2.2 1,992,417 - -3.6 1,992,417 - -1.6 1,992,427 - -1.6 1,992,495 + +0.1 1,992,584 + -0.9 1,992,663 + SO1895 0.17 -2.2 1,992,826 + SO1895 0.36 -3.2 1,992,968 + SO1895 0.53 -2.8 1,992,980 + SO1895 0.54 -1.9 1,992,980 + SO1895 0.54 -1.4 1,993,056 + SO1895 0.63 -1.4 1,993,228 + SO1895 0.83 -3.7 1,993,261 + SO1895 0.86 -2.4 1,993,312 + -1.6 1,993,355 + -0.9
Or see this region's nucleotide sequence