Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiI and SO1532 are separated by 132 nucleotides SO1532 and SO1533 are separated by 176 nucleotides SO1533 and SO1534 are separated by 4 nucleotides SO1534 and SO1535 are separated by 95 nucleotides
SO1531: thiI - thiamine biosynthesis protein ThiI (NCBI ptt file), at 1,606,408 to 1,607,862
thiI
SO1532: SO1532 - hypothetical protein (NCBI ptt file), at 1,607,995 to 1,608,228
SO1532
SO1533: SO1533 - glycine cleavage system transcriptional activator, putative (NCBI ptt file), at 1,608,405 to 1,609,316
SO1533
SO1534: SO1534 - conserved hypothetical protein (NCBI ptt file), at 1,609,321 to 1,609,968
SO1534
SO1535: SO1535 - conserved hypothetical protein (NCBI ptt file), at 1,610,064 to 1,610,456
SO1535
Position (kb)
1608
1609
1610 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1607.411 kb on - strand, within thiI at 1607.427 kb on + strand, within thiI at 1607.435 kb on - strand, within thiI at 1607.484 kb on + strand, within thiI at 1607.484 kb on + strand, within thiI at 1607.492 kb on - strand, within thiI at 1607.498 kb on - strand, within thiI at 1607.688 kb on + strand, within thiI at 1607.796 kb on - strand at 1607.870 kb on + strand at 1607.914 kb on + strand at 1607.967 kb on + strand at 1608.030 kb on - strand, within SO1532 at 1608.126 kb on - strand, within SO1532 at 1608.154 kb on + strand, within SO1532 at 1608.242 kb on - strand at 1608.242 kb on - strand at 1608.286 kb on - strand at 1608.506 kb on + strand, within SO1533 at 1608.663 kb on + strand, within SO1533 at 1608.859 kb on + strand, within SO1533 at 1608.859 kb on + strand, within SO1533 at 1608.859 kb on + strand, within SO1533 at 1608.918 kb on + strand, within SO1533 at 1608.939 kb on - strand, within SO1533 at 1609.118 kb on + strand, within SO1533 at 1609.126 kb on - strand, within SO1533 at 1609.126 kb on - strand, within SO1533 at 1609.126 kb on - strand, within SO1533 at 1609.190 kb on - strand, within SO1533 at 1609.208 kb on - strand, within SO1533 at 1609.238 kb on + strand at 1609.238 kb on + strand at 1609.238 kb on + strand at 1609.246 kb on - strand at 1609.270 kb on - strand at 1609.320 kb on - strand at 1609.408 kb on - strand, within SO1534 at 1609.441 kb on + strand, within SO1534 at 1609.450 kb on - strand, within SO1534 at 1609.450 kb on - strand, within SO1534 at 1609.536 kb on - strand, within SO1534 at 1609.599 kb on + strand, within SO1534 at 1609.620 kb on - strand, within SO1534 at 1609.737 kb on + strand, within SO1534 at 1609.737 kb on + strand, within SO1534 at 1609.745 kb on - strand, within SO1534 at 1609.750 kb on - strand, within SO1534 at 1609.758 kb on - strand, within SO1534 at 1609.783 kb on - strand, within SO1534 at 1609.784 kb on + strand, within SO1534 at 1609.849 kb on - strand, within SO1534 at 1609.925 kb on + strand at 1610.048 kb on - strand at 1610.158 kb on + strand, within SO1535 at 1610.166 kb on - strand, within SO1535 at 1610.212 kb on + strand, within SO1535
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 1,607,411 - thiI SO1531 0.69 +0.3 1,607,427 + thiI SO1531 0.70 -0.3 1,607,435 - thiI SO1531 0.71 -1.4 1,607,484 + thiI SO1531 0.74 -0.8 1,607,484 + thiI SO1531 0.74 -0.7 1,607,492 - thiI SO1531 0.75 -0.9 1,607,498 - thiI SO1531 0.75 -0.8 1,607,688 + thiI SO1531 0.88 -0.3 1,607,796 - -1.1 1,607,870 + +0.4 1,607,914 + -0.3 1,607,967 + +0.7 1,608,030 - SO1532 0.15 +0.9 1,608,126 - SO1532 0.56 -0.7 1,608,154 + SO1532 0.68 +1.2 1,608,242 - +0.5 1,608,242 - -0.5 1,608,286 - -0.3 1,608,506 + SO1533 0.11 -3.8 1,608,663 + SO1533 0.28 +0.3 1,608,859 + SO1533 0.50 -3.5 1,608,859 + SO1533 0.50 -1.3 1,608,859 + SO1533 0.50 -3.1 1,608,918 + SO1533 0.56 -1.0 1,608,939 - SO1533 0.59 -1.7 1,609,118 + SO1533 0.78 -2.9 1,609,126 - SO1533 0.79 -4.9 1,609,126 - SO1533 0.79 -2.7 1,609,126 - SO1533 0.79 -2.7 1,609,190 - SO1533 0.86 -1.3 1,609,208 - SO1533 0.88 -0.7 1,609,238 + -1.0 1,609,238 + -3.3 1,609,238 + -2.7 1,609,246 - -2.6 1,609,270 - -2.5 1,609,320 - -0.3 1,609,408 - SO1534 0.13 +0.1 1,609,441 + SO1534 0.19 -0.8 1,609,450 - SO1534 0.20 -0.5 1,609,450 - SO1534 0.20 -0.8 1,609,536 - SO1534 0.33 +0.1 1,609,599 + SO1534 0.43 -0.4 1,609,620 - SO1534 0.46 -0.2 1,609,737 + SO1534 0.64 -0.5 1,609,737 + SO1534 0.64 -2.1 1,609,745 - SO1534 0.65 +0.1 1,609,750 - SO1534 0.66 -0.5 1,609,758 - SO1534 0.67 -0.7 1,609,783 - SO1534 0.71 -0.3 1,609,784 + SO1534 0.71 -1.0 1,609,849 - SO1534 0.81 +0.1 1,609,925 + -0.3 1,610,048 - +0.7 1,610,158 + SO1535 0.24 -0.8 1,610,166 - SO1535 0.26 +0.1 1,610,212 + SO1535 0.38 -0.9
Or see this region's nucleotide sequence