Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobT and cobS are separated by 106 nucleotides cobS and cobU overlap by 4 nucleotides cobU and cobQ are separated by 211 nucleotides
SO1035: cobT - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (NCBI ptt file), at 1,074,417 to 1,075,469
cobT
SO1036: cobS - cobalamin 5'-phosphate synthase (NCBI ptt file), at 1,075,576 to 1,076,364
cobS
SO1037: cobU - cobinamide kinase/cobinamide phosphate guanylyltransferase (NCBI ptt file), at 1,076,361 to 1,076,966
cobU
SO1038: cobQ - cobyric acid synthase CobQ (NCBI ptt file), at 1,077,178 to 1,078,710
cobQ
Position (kb)
1076
1077 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1075.362 kb on + strand, within cobT at 1075.459 kb on - strand at 1075.461 kb on + strand at 1075.464 kb on + strand at 1075.469 kb on - strand at 1075.694 kb on - strand, within cobS at 1075.742 kb on - strand, within cobS at 1075.849 kb on + strand, within cobS at 1075.884 kb on - strand, within cobS at 1075.885 kb on + strand, within cobS at 1076.038 kb on + strand, within cobS at 1076.071 kb on - strand, within cobS at 1076.086 kb on - strand, within cobS at 1076.168 kb on + strand, within cobS at 1076.230 kb on + strand, within cobS at 1076.248 kb on - strand, within cobS at 1076.330 kb on + strand at 1076.373 kb on + strand at 1076.463 kb on - strand, within cobU at 1076.626 kb on + strand, within cobU at 1076.626 kb on + strand, within cobU at 1076.626 kb on + strand, within cobU at 1076.634 kb on - strand, within cobU at 1076.754 kb on - strand, within cobU at 1076.826 kb on + strand, within cobU at 1076.898 kb on + strand, within cobU at 1076.921 kb on + strand at 1076.961 kb on + strand at 1077.070 kb on + strand at 1077.115 kb on - strand at 1077.142 kb on - strand at 1077.194 kb on + strand at 1077.194 kb on + strand at 1077.194 kb on + strand at 1077.194 kb on + strand at 1077.194 kb on - strand at 1077.202 kb on - strand at 1077.202 kb on - strand at 1077.302 kb on - strand at 1077.318 kb on - strand at 1077.413 kb on - strand, within cobQ at 1077.438 kb on + strand, within cobQ at 1077.446 kb on - strand, within cobQ at 1077.446 kb on - strand, within cobQ at 1077.446 kb on - strand, within cobQ at 1077.446 kb on - strand, within cobQ at 1077.446 kb on - strand, within cobQ at 1077.510 kb on + strand, within cobQ at 1077.511 kb on - strand, within cobQ at 1077.538 kb on - strand, within cobQ at 1077.538 kb on - strand, within cobQ at 1077.582 kb on - strand, within cobQ at 1077.797 kb on - strand, within cobQ at 1077.912 kb on + strand, within cobQ at 1077.937 kb on + strand, within cobQ
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 1,075,362 + cobT SO1035 0.90 +0.9 1,075,459 - -1.8 1,075,461 + -0.5 1,075,464 + +1.0 1,075,469 - -0.9 1,075,694 - cobS SO1036 0.15 -2.2 1,075,742 - cobS SO1036 0.21 -1.2 1,075,849 + cobS SO1036 0.35 -1.2 1,075,884 - cobS SO1036 0.39 -1.7 1,075,885 + cobS SO1036 0.39 -0.2 1,076,038 + cobS SO1036 0.59 -0.6 1,076,071 - cobS SO1036 0.63 -1.0 1,076,086 - cobS SO1036 0.65 -1.7 1,076,168 + cobS SO1036 0.75 -0.8 1,076,230 + cobS SO1036 0.83 -0.6 1,076,248 - cobS SO1036 0.85 +0.3 1,076,330 + +4.9 1,076,373 + -0.2 1,076,463 - cobU SO1037 0.17 -2.0 1,076,626 + cobU SO1037 0.44 -2.5 1,076,626 + cobU SO1037 0.44 -0.8 1,076,626 + cobU SO1037 0.44 +1.0 1,076,634 - cobU SO1037 0.45 -2.8 1,076,754 - cobU SO1037 0.65 -2.4 1,076,826 + cobU SO1037 0.77 -0.4 1,076,898 + cobU SO1037 0.89 -0.5 1,076,921 + -0.3 1,076,961 + +1.3 1,077,070 + +0.4 1,077,115 - -3.5 1,077,142 - -3.2 1,077,194 + +1.3 1,077,194 + -0.2 1,077,194 + +0.4 1,077,194 + -1.7 1,077,194 - -3.8 1,077,202 - -3.0 1,077,202 - -2.9 1,077,302 - -0.8 1,077,318 - -3.0 1,077,413 - cobQ SO1038 0.15 -1.7 1,077,438 + cobQ SO1038 0.17 -0.8 1,077,446 - cobQ SO1038 0.17 -2.3 1,077,446 - cobQ SO1038 0.17 -2.3 1,077,446 - cobQ SO1038 0.17 -1.4 1,077,446 - cobQ SO1038 0.17 -1.5 1,077,446 - cobQ SO1038 0.17 -0.9 1,077,510 + cobQ SO1038 0.22 +0.5 1,077,511 - cobQ SO1038 0.22 -2.1 1,077,538 - cobQ SO1038 0.23 -1.7 1,077,538 - cobQ SO1038 0.23 -1.8 1,077,582 - cobQ SO1038 0.26 +0.2 1,077,797 - cobQ SO1038 0.40 -1.8 1,077,912 + cobQ SO1038 0.48 -0.1 1,077,937 + cobQ SO1038 0.50 -0.7
Or see this region's nucleotide sequence