Experiment: Gly-DL-Asp (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdhR and aceE are separated by 81 nucleotides aceE and aceF are separated by 12 nucleotides
SO0423: pdhR - pyruvate dehydrogenase complex repressor (NCBI ptt file), at 437,566 to 438,318
pdhR
SO0424: aceE - pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase (NCBI ptt file), at 438,400 to 441,066
aceE
SO0425: aceF - pyruvate dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase (NCBI ptt file), at 441,079 to 443,112
aceF
Position (kb) 438
439
440
441
442 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 437.430 kb on + strand at 437.438 kb on - strand at 437.645 kb on + strand, within pdhR at 437.645 kb on + strand, within pdhR at 437.645 kb on + strand, within pdhR at 437.653 kb on - strand, within pdhR at 437.653 kb on - strand, within pdhR at 437.726 kb on - strand, within pdhR at 437.924 kb on - strand, within pdhR at 437.964 kb on + strand, within pdhR at 438.034 kb on + strand, within pdhR at 438.095 kb on - strand, within pdhR at 438.156 kb on + strand, within pdhR at 438.226 kb on + strand, within pdhR at 438.268 kb on + strand at 438.493 kb on - strand at 438.505 kb on - strand at 438.576 kb on + strand at 438.718 kb on + strand, within aceE at 438.915 kb on + strand, within aceE at 438.982 kb on + strand, within aceE at 439.112 kb on + strand, within aceE at 439.212 kb on + strand, within aceE at 439.256 kb on + strand, within aceE at 439.504 kb on + strand, within aceE at 439.513 kb on + strand, within aceE at 439.513 kb on + strand, within aceE at 439.513 kb on + strand, within aceE at 439.513 kb on + strand, within aceE at 439.513 kb on + strand, within aceE at 439.513 kb on + strand, within aceE at 439.521 kb on - strand, within aceE at 439.521 kb on - strand, within aceE at 439.773 kb on + strand, within aceE at 439.781 kb on - strand, within aceE at 439.781 kb on - strand, within aceE at 439.906 kb on + strand, within aceE at 439.917 kb on + strand, within aceE at 439.917 kb on + strand, within aceE at 439.976 kb on + strand, within aceE at 440.246 kb on - strand, within aceE at 440.329 kb on + strand, within aceE at 440.346 kb on + strand, within aceE at 440.346 kb on + strand, within aceE at 440.521 kb on - strand, within aceE at 440.660 kb on + strand, within aceE at 440.758 kb on - strand, within aceE at 440.761 kb on + strand, within aceE at 440.854 kb on - strand at 440.869 kb on + strand at 440.869 kb on + strand at 440.877 kb on - strand at 440.877 kb on - strand at 440.879 kb on - strand at 440.903 kb on + strand at 441.048 kb on - strand at 441.151 kb on + strand at 441.298 kb on - strand, within aceF at 441.736 kb on - strand, within aceF at 441.875 kb on + strand, within aceF at 441.875 kb on + strand, within aceF at 441.875 kb on + strand, within aceF at 441.883 kb on - strand, within aceF at 441.962 kb on - strand, within aceF at 441.970 kb on - strand, within aceF
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (C) remove 437,430 + +1.0 437,438 - -1.0 437,645 + pdhR SO0423 0.10 -1.7 437,645 + pdhR SO0423 0.10 -0.9 437,645 + pdhR SO0423 0.10 -1.9 437,653 - pdhR SO0423 0.12 -3.7 437,653 - pdhR SO0423 0.12 -0.7 437,726 - pdhR SO0423 0.21 -1.2 437,924 - pdhR SO0423 0.48 -1.1 437,964 + pdhR SO0423 0.53 +1.3 438,034 + pdhR SO0423 0.62 -0.0 438,095 - pdhR SO0423 0.70 -2.4 438,156 + pdhR SO0423 0.78 -0.1 438,226 + pdhR SO0423 0.88 +0.9 438,268 + +0.1 438,493 - -4.0 438,505 - -2.4 438,576 + -1.1 438,718 + aceE SO0424 0.12 -1.7 438,915 + aceE SO0424 0.19 -3.7 438,982 + aceE SO0424 0.22 -1.7 439,112 + aceE SO0424 0.27 -2.7 439,212 + aceE SO0424 0.30 -1.7 439,256 + aceE SO0424 0.32 -1.7 439,504 + aceE SO0424 0.41 -0.7 439,513 + aceE SO0424 0.42 -1.9 439,513 + aceE SO0424 0.42 -1.7 439,513 + aceE SO0424 0.42 -1.4 439,513 + aceE SO0424 0.42 -2.7 439,513 + aceE SO0424 0.42 -0.7 439,513 + aceE SO0424 0.42 -1.1 439,521 - aceE SO0424 0.42 -2.7 439,521 - aceE SO0424 0.42 -1.1 439,773 + aceE SO0424 0.51 -0.1 439,781 - aceE SO0424 0.52 -1.7 439,781 - aceE SO0424 0.52 -0.7 439,906 + aceE SO0424 0.56 -0.7 439,917 + aceE SO0424 0.57 -1.1 439,917 + aceE SO0424 0.57 -2.4 439,976 + aceE SO0424 0.59 -1.9 440,246 - aceE SO0424 0.69 -0.7 440,329 + aceE SO0424 0.72 -3.4 440,346 + aceE SO0424 0.73 -2.2 440,346 + aceE SO0424 0.73 -1.1 440,521 - aceE SO0424 0.80 -1.5 440,660 + aceE SO0424 0.85 -0.1 440,758 - aceE SO0424 0.88 -1.4 440,761 + aceE SO0424 0.89 -1.4 440,854 - -3.5 440,869 + -3.0 440,869 + -1.7 440,877 - -2.2 440,877 - -2.8 440,879 - -0.7 440,903 + -2.2 441,048 - -3.4 441,151 + -1.9 441,298 - aceF SO0425 0.11 -1.9 441,736 - aceF SO0425 0.32 -2.2 441,875 + aceF SO0425 0.39 +0.9 441,875 + aceF SO0425 0.39 -0.9 441,875 + aceF SO0425 0.39 -3.3 441,883 - aceF SO0425 0.40 -0.9 441,962 - aceF SO0425 0.43 -3.1 441,970 - aceF SO0425 0.44 -2.1
Or see this region's nucleotide sequence