Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO_A0009 and SOA0010 overlap by 14 nucleotides SOA0010 and SOA0011 are separated by 141 nucleotides SOA0011 and SO_A0174 are separated by 34 nucleotides SO_A0174 and umuC are separated by 129 nucleotides
SO_A0009: SO_A0009 - hypothetical protein (NCBI), at 8,573 to 8,818
_A0009
SOA0010: SOA0010 - conserved hypothetical protein (NCBI ptt file), at 8,805 to 9,323
SOA0010
SOA0011: SOA0011 - conserved hypothetical protein (NCBI ptt file), at 9,465 to 9,707
SOA0011
SO_A0174: SO_A0174 - hypothetical protein (NCBI), at 9,742 to 9,948
_A0174
SOA0012: umuC - umuC protein (NCBI ptt file), at 10,078 to 11,334
umuC
Position (kb)
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 8.742 kb on + strand, within SO_A0009 at 8.742 kb on + strand, within SO_A0009 at 8.826 kb on - strand at 8.877 kb on + strand, within SOA0010 at 8.913 kb on - strand, within SOA0010 at 8.939 kb on + strand, within SOA0010 at 8.978 kb on + strand, within SOA0010 at 9.184 kb on + strand, within SOA0010 at 9.504 kb on + strand, within SOA0011 at 9.578 kb on - strand, within SOA0011 at 9.671 kb on + strand, within SOA0011 at 9.671 kb on + strand, within SOA0011 at 9.671 kb on + strand, within SOA0011 at 9.671 kb on + strand, within SOA0011 at 9.671 kb on + strand, within SOA0011 at 9.679 kb on - strand, within SOA0011 at 9.679 kb on - strand, within SOA0011 at 9.679 kb on - strand, within SOA0011 at 9.679 kb on - strand, within SOA0011 at 9.679 kb on - strand, within SOA0011 at 9.679 kb on - strand, within SOA0011 at 9.679 kb on - strand, within SOA0011 at 9.679 kb on - strand, within SOA0011 at 9.692 kb on - strand at 9.692 kb on - strand at 9.692 kb on - strand at 9.694 kb on - strand at 9.756 kb on + strand at 9.781 kb on + strand, within SO_A0174 at 9.800 kb on + strand, within SO_A0174 at 9.868 kb on + strand, within SO_A0174 at 9.922 kb on - strand, within SO_A0174 at 10.003 kb on + strand at 10.014 kb on + strand at 10.081 kb on + strand at 10.096 kb on - strand at 10.115 kb on - strand at 10.130 kb on + strand at 10.131 kb on + strand at 10.173 kb on - strand at 10.240 kb on + strand, within umuC at 10.258 kb on + strand, within umuC at 10.272 kb on + strand, within umuC at 10.295 kb on - strand, within umuC at 10.324 kb on + strand, within umuC at 10.375 kb on + strand, within umuC at 10.416 kb on - strand, within umuC at 10.427 kb on - strand, within umuC at 10.449 kb on + strand, within umuC at 10.491 kb on - strand, within umuC at 10.526 kb on + strand, within umuC at 10.534 kb on - strand, within umuC at 10.534 kb on - strand, within umuC at 10.693 kb on + strand, within umuC at 10.700 kb on - strand, within umuC at 10.720 kb on - strand, within umuC at 10.785 kb on - strand, within umuC at 10.791 kb on - strand, within umuC at 10.819 kb on + strand, within umuC at 10.827 kb on + strand, within umuC at 10.835 kb on - strand, within umuC at 10.899 kb on + strand, within umuC at 10.899 kb on + strand, within umuC at 10.899 kb on + strand, within umuC at 10.899 kb on + strand, within umuC at 10.899 kb on + strand, within umuC at 10.907 kb on - strand, within umuC at 10.907 kb on - strand, within umuC at 10.907 kb on - strand, within umuC at 10.907 kb on - strand, within umuC at 10.907 kb on - strand, within umuC at 10.907 kb on - strand, within umuC
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 8,742 + SO_A0009 0.69 +1.7 8,742 + SO_A0009 0.69 +0.8 8,826 - +1.9 8,877 + SOA0010 0.14 -1.6 8,913 - SOA0010 0.21 -0.7 8,939 + SOA0010 0.26 +0.5 8,978 + SOA0010 0.33 +0.6 9,184 + SOA0010 0.73 -3.2 9,504 + SOA0011 0.16 -2.8 9,578 - SOA0011 0.47 +3.2 9,671 + SOA0011 0.85 +0.7 9,671 + SOA0011 0.85 +0.8 9,671 + SOA0011 0.85 +1.0 9,671 + SOA0011 0.85 +2.4 9,671 + SOA0011 0.85 -1.3 9,679 - SOA0011 0.88 +2.3 9,679 - SOA0011 0.88 +3.7 9,679 - SOA0011 0.88 +3.7 9,679 - SOA0011 0.88 +5.8 9,679 - SOA0011 0.88 +2.4 9,679 - SOA0011 0.88 +2.6 9,679 - SOA0011 0.88 +2.6 9,679 - SOA0011 0.88 +3.0 9,692 - +3.0 9,692 - +0.5 9,692 - +1.5 9,694 - +3.2 9,756 + -0.3 9,781 + SO_A0174 0.19 +0.7 9,800 + SO_A0174 0.28 +3.2 9,868 + SO_A0174 0.61 +0.2 9,922 - SO_A0174 0.87 +1.1 10,003 + -2.2 10,014 + -1.9 10,081 + +1.6 10,096 - +2.1 10,115 - +0.9 10,130 + +1.2 10,131 + -0.1 10,173 - +2.1 10,240 + umuC SOA0012 0.13 -2.2 10,258 + umuC SOA0012 0.14 +1.7 10,272 + umuC SOA0012 0.15 +0.2 10,295 - umuC SOA0012 0.17 +0.9 10,324 + umuC SOA0012 0.20 -1.6 10,375 + umuC SOA0012 0.24 -0.2 10,416 - umuC SOA0012 0.27 -0.2 10,427 - umuC SOA0012 0.28 +0.6 10,449 + umuC SOA0012 0.30 +0.7 10,491 - umuC SOA0012 0.33 -0.3 10,526 + umuC SOA0012 0.36 -0.7 10,534 - umuC SOA0012 0.36 +0.4 10,534 - umuC SOA0012 0.36 +1.0 10,693 + umuC SOA0012 0.49 +0.2 10,700 - umuC SOA0012 0.49 +1.7 10,720 - umuC SOA0012 0.51 -1.1 10,785 - umuC SOA0012 0.56 +2.6 10,791 - umuC SOA0012 0.57 -1.0 10,819 + umuC SOA0012 0.59 +1.2 10,827 + umuC SOA0012 0.60 -1.6 10,835 - umuC SOA0012 0.60 +1.4 10,899 + umuC SOA0012 0.65 -0.4 10,899 + umuC SOA0012 0.65 -0.1 10,899 + umuC SOA0012 0.65 -0.5 10,899 + umuC SOA0012 0.65 -1.4 10,899 + umuC SOA0012 0.65 -1.1 10,907 - umuC SOA0012 0.66 +1.4 10,907 - umuC SOA0012 0.66 +1.5 10,907 - umuC SOA0012 0.66 +0.0 10,907 - umuC SOA0012 0.66 -0.8 10,907 - umuC SOA0012 0.66 +2.5 10,907 - umuC SOA0012 0.66 +1.1
Or see this region's nucleotide sequence