Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4723 and SO4724 are separated by 16 nucleotides SO4724 and SO4725 are separated by 81 nucleotides SO4725 and mutM are separated by 24 nucleotides
SO4723: SO4723 - molybdopterin biosynthesis MoeA protein, putative (NCBI ptt file), at 4,927,038 to 4,928,837
SO4723
SO4724: SO4724 - molybdenum cofactor biosynthesis protein A, putative (NCBI ptt file), at 4,928,854 to 4,929,837
SO4724
SO4725: SO4725 - conserved hypothetical protein (NCBI ptt file), at 4,929,919 to 4,930,374
SO4725
SO4726: mutM - formamidopyrimidine-DNA glycosylase (NCBI ptt file), at 4,930,399 to 4,931,214
mutM
Position (kb)
4928
4929
4930 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 4927.987 kb on + strand, within SO4723 at 4927.995 kb on - strand, within SO4723 at 4928.019 kb on - strand, within SO4723 at 4928.031 kb on + strand, within SO4723 at 4928.063 kb on + strand, within SO4723 at 4928.084 kb on - strand, within SO4723 at 4928.084 kb on - strand, within SO4723 at 4928.110 kb on + strand, within SO4723 at 4928.124 kb on - strand, within SO4723 at 4928.133 kb on - strand, within SO4723 at 4928.143 kb on + strand, within SO4723 at 4928.167 kb on - strand, within SO4723 at 4928.228 kb on + strand, within SO4723 at 4928.228 kb on + strand, within SO4723 at 4928.236 kb on + strand, within SO4723 at 4928.236 kb on - strand, within SO4723 at 4928.236 kb on - strand, within SO4723 at 4928.236 kb on - strand, within SO4723 at 4928.244 kb on - strand, within SO4723 at 4928.263 kb on + strand, within SO4723 at 4928.264 kb on - strand, within SO4723 at 4928.310 kb on + strand, within SO4723 at 4928.393 kb on + strand, within SO4723 at 4928.485 kb on - strand, within SO4723 at 4928.662 kb on + strand at 4928.741 kb on + strand at 4928.846 kb on - strand at 4928.919 kb on - strand at 4929.016 kb on + strand, within SO4724 at 4929.051 kb on + strand, within SO4724 at 4929.069 kb on + strand, within SO4724 at 4929.129 kb on - strand, within SO4724 at 4929.149 kb on + strand, within SO4724 at 4929.149 kb on + strand, within SO4724 at 4929.222 kb on + strand, within SO4724 at 4929.230 kb on - strand, within SO4724 at 4929.235 kb on - strand, within SO4724 at 4929.289 kb on + strand, within SO4724 at 4929.389 kb on - strand, within SO4724 at 4929.447 kb on + strand, within SO4724 at 4929.470 kb on - strand, within SO4724 at 4929.501 kb on + strand, within SO4724 at 4929.557 kb on - strand, within SO4724 at 4929.557 kb on - strand, within SO4724 at 4929.559 kb on - strand, within SO4724 at 4929.592 kb on - strand, within SO4724 at 4929.595 kb on + strand, within SO4724 at 4929.621 kb on + strand, within SO4724 at 4929.621 kb on + strand, within SO4724 at 4929.629 kb on - strand, within SO4724 at 4929.629 kb on - strand, within SO4724 at 4929.665 kb on + strand, within SO4724 at 4929.763 kb on - strand at 4929.767 kb on + strand at 4929.838 kb on + strand at 4929.838 kb on + strand at 4929.846 kb on + strand at 4929.854 kb on - strand at 4929.854 kb on - strand at 4929.854 kb on - strand at 4929.929 kb on + strand at 4929.929 kb on + strand at 4929.929 kb on + strand at 4929.929 kb on + strand at 4929.937 kb on - strand at 4929.937 kb on - strand at 4929.937 kb on - strand at 4929.984 kb on + strand, within SO4725 at 4930.079 kb on + strand, within SO4725 at 4930.079 kb on + strand, within SO4725 at 4930.079 kb on + strand, within SO4725 at 4930.079 kb on + strand, within SO4725 at 4930.079 kb on + strand, within SO4725 at 4930.085 kb on - strand, within SO4725 at 4930.087 kb on - strand, within SO4725 at 4930.087 kb on - strand, within SO4725 at 4930.126 kb on + strand, within SO4725 at 4930.126 kb on + strand, within SO4725 at 4930.134 kb on - strand, within SO4725 at 4930.162 kb on + strand, within SO4725 at 4930.163 kb on + strand, within SO4725 at 4930.170 kb on - strand, within SO4725 at 4930.170 kb on - strand, within SO4725 at 4930.171 kb on - strand, within SO4725 at 4930.171 kb on - strand, within SO4725 at 4930.198 kb on - strand, within SO4725 at 4930.200 kb on - strand, within SO4725 at 4930.200 kb on - strand, within SO4725 at 4930.292 kb on - strand, within SO4725 at 4930.315 kb on - strand, within SO4725 at 4930.326 kb on + strand, within SO4725 at 4930.344 kb on + strand at 4930.434 kb on + strand at 4930.505 kb on - strand, within mutM at 4930.628 kb on + strand, within mutM at 4930.636 kb on - strand, within mutM at 4930.636 kb on - strand, within mutM at 4930.642 kb on + strand, within mutM at 4930.665 kb on - strand, within mutM at 4930.806 kb on - strand, within mutM at 4930.820 kb on - strand, within mutM
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,927,987 + SO4723 0.53 -2.8 4,927,995 - SO4723 0.53 -1.5 4,928,019 - SO4723 0.55 -2.3 4,928,031 + SO4723 0.55 -1.1 4,928,063 + SO4723 0.57 +0.8 4,928,084 - SO4723 0.58 -2.4 4,928,084 - SO4723 0.58 -1.0 4,928,110 + SO4723 0.60 +1.4 4,928,124 - SO4723 0.60 -1.0 4,928,133 - SO4723 0.61 +1.4 4,928,143 + SO4723 0.61 +0.5 4,928,167 - SO4723 0.63 -0.3 4,928,228 + SO4723 0.66 -2.5 4,928,228 + SO4723 0.66 -1.9 4,928,236 + SO4723 0.67 -0.3 4,928,236 - SO4723 0.67 -0.8 4,928,236 - SO4723 0.67 +0.1 4,928,236 - SO4723 0.67 -2.9 4,928,244 - SO4723 0.67 -3.0 4,928,263 + SO4723 0.68 -1.3 4,928,264 - SO4723 0.68 -1.1 4,928,310 + SO4723 0.71 -1.4 4,928,393 + SO4723 0.75 -1.9 4,928,485 - SO4723 0.80 -3.1 4,928,662 + +2.4 4,928,741 + -0.6 4,928,846 - -0.0 4,928,919 - -2.6 4,929,016 + SO4724 0.16 +0.3 4,929,051 + SO4724 0.20 -0.9 4,929,069 + SO4724 0.22 +0.0 4,929,129 - SO4724 0.28 +1.0 4,929,149 + SO4724 0.30 -1.0 4,929,149 + SO4724 0.30 -1.6 4,929,222 + SO4724 0.37 -1.7 4,929,230 - SO4724 0.38 +0.3 4,929,235 - SO4724 0.39 +2.6 4,929,289 + SO4724 0.44 -0.3 4,929,389 - SO4724 0.54 -2.2 4,929,447 + SO4724 0.60 -0.6 4,929,470 - SO4724 0.63 -0.5 4,929,501 + SO4724 0.66 +0.2 4,929,557 - SO4724 0.71 +1.3 4,929,557 - SO4724 0.71 -0.7 4,929,559 - SO4724 0.72 +0.1 4,929,592 - SO4724 0.75 -1.2 4,929,595 + SO4724 0.75 +0.1 4,929,621 + SO4724 0.78 +1.3 4,929,621 + SO4724 0.78 -0.7 4,929,629 - SO4724 0.79 -1.7 4,929,629 - SO4724 0.79 +0.3 4,929,665 + SO4724 0.82 -0.1 4,929,763 - -2.9 4,929,767 + -1.2 4,929,838 + -0.6 4,929,838 + -1.7 4,929,846 + +1.1 4,929,854 - +2.8 4,929,854 - -2.2 4,929,854 - +0.4 4,929,929 + +0.3 4,929,929 + +0.6 4,929,929 + +1.6 4,929,929 + +2.3 4,929,937 - -1.1 4,929,937 - +0.8 4,929,937 - -4.3 4,929,984 + SO4725 0.14 +0.9 4,930,079 + SO4725 0.35 +2.3 4,930,079 + SO4725 0.35 -2.2 4,930,079 + SO4725 0.35 -0.5 4,930,079 + SO4725 0.35 +1.8 4,930,079 + SO4725 0.35 +3.5 4,930,085 - SO4725 0.36 +0.6 4,930,087 - SO4725 0.37 -2.7 4,930,087 - SO4725 0.37 +0.8 4,930,126 + SO4725 0.45 +3.0 4,930,126 + SO4725 0.45 +0.6 4,930,134 - SO4725 0.47 -0.6 4,930,162 + SO4725 0.53 +2.4 4,930,163 + SO4725 0.54 +0.4 4,930,170 - SO4725 0.55 +0.1 4,930,170 - SO4725 0.55 -1.1 4,930,171 - SO4725 0.55 +1.2 4,930,171 - SO4725 0.55 +0.3 4,930,198 - SO4725 0.61 -0.2 4,930,200 - SO4725 0.62 +1.2 4,930,200 - SO4725 0.62 -0.9 4,930,292 - SO4725 0.82 -1.6 4,930,315 - SO4725 0.87 +5.5 4,930,326 + SO4725 0.89 -2.2 4,930,344 + +0.4 4,930,434 + -0.6 4,930,505 - mutM SO4726 0.13 +0.2 4,930,628 + mutM SO4726 0.28 +0.7 4,930,636 - mutM SO4726 0.29 -1.1 4,930,636 - mutM SO4726 0.29 +1.3 4,930,642 + mutM SO4726 0.30 +1.0 4,930,665 - mutM SO4726 0.33 -1.7 4,930,806 - mutM SO4726 0.50 +1.0 4,930,820 - mutM SO4726 0.52 +1.3
Or see this region's nucleotide sequence