Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdtA and SO4677 are separated by 15 nucleotides SO4677 and SO4678 are separated by 88 nucleotides SO4678 and SO4679 are separated by 52 nucleotides
SO4676: kdtA - 3-deoxy-D-manno-octulosonic-acid (KDO) transferase (NCBI ptt file), at 4,874,417 to 4,875,691
kdtA
SO4677: SO4677 - conserved hypothetical protein (NCBI ptt file), at 4,875,707 to 4,876,459
SO4677
SO4678: SO4678 - heptosyl transferase, glycosyltransferase family 9 protein (NCBI ptt file), at 4,876,548 to 4,877,591
SO4678
SO4679: SO4679 - glycosyl transferase, group 1 family protein (NCBI ptt file), at 4,877,644 to 4,878,729
SO4679
Position (kb)
4876
4877
4878 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 4875.686 kb on + strand at 4875.686 kb on + strand at 4875.686 kb on + strand at 4875.686 kb on + strand at 4875.686 kb on + strand at 4875.686 kb on + strand at 4875.686 kb on + strand at 4875.686 kb on + strand at 4875.694 kb on - strand at 4875.694 kb on - strand at 4875.694 kb on - strand at 4875.694 kb on - strand at 4875.694 kb on - strand at 4875.713 kb on - strand at 4875.743 kb on - strand at 4876.518 kb on - strand at 4876.539 kb on - strand at 4876.539 kb on - strand at 4876.639 kb on - strand at 4876.639 kb on - strand at 4876.720 kb on + strand, within SO4678 at 4876.790 kb on - strand, within SO4678 at 4876.798 kb on + strand, within SO4678 at 4876.948 kb on + strand, within SO4678 at 4876.959 kb on - strand, within SO4678 at 4877.023 kb on - strand, within SO4678 at 4877.038 kb on - strand, within SO4678 at 4877.060 kb on - strand, within SO4678 at 4877.137 kb on - strand, within SO4678 at 4877.165 kb on + strand, within SO4678 at 4877.173 kb on - strand, within SO4678 at 4877.173 kb on - strand, within SO4678 at 4877.179 kb on + strand, within SO4678 at 4877.187 kb on - strand, within SO4678 at 4877.303 kb on + strand, within SO4678 at 4877.366 kb on + strand, within SO4678 at 4877.396 kb on + strand, within SO4678 at 4877.404 kb on - strand, within SO4678 at 4877.416 kb on + strand, within SO4678 at 4877.424 kb on - strand, within SO4678 at 4877.442 kb on - strand, within SO4678 at 4877.457 kb on - strand, within SO4678 at 4877.460 kb on + strand, within SO4678 at 4877.467 kb on + strand, within SO4678 at 4877.467 kb on + strand, within SO4678 at 4877.544 kb on - strand at 4877.585 kb on + strand at 4877.585 kb on + strand at 4877.587 kb on + strand at 4877.595 kb on + strand at 4877.659 kb on + strand at 4877.667 kb on - strand at 4877.677 kb on - strand at 4877.677 kb on - strand at 4877.702 kb on - strand at 4877.732 kb on + strand at 4877.795 kb on - strand, within SO4679 at 4878.131 kb on - strand, within SO4679 at 4878.216 kb on - strand, within SO4679 at 4878.299 kb on + strand, within SO4679 at 4878.307 kb on - strand, within SO4679 at 4878.331 kb on + strand, within SO4679 at 4878.334 kb on - strand, within SO4679 at 4878.334 kb on - strand, within SO4679 at 4878.349 kb on - strand, within SO4679 at 4878.520 kb on + strand, within SO4679 at 4878.520 kb on + strand, within SO4679 at 4878.525 kb on + strand, within SO4679 at 4878.526 kb on - strand, within SO4679
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,875,686 + -1.3 4,875,686 + +0.8 4,875,686 + +0.1 4,875,686 + -0.7 4,875,686 + +1.1 4,875,686 + +4.1 4,875,686 + +0.4 4,875,686 + -0.8 4,875,694 - -1.2 4,875,694 - -1.7 4,875,694 - -1.6 4,875,694 - +0.9 4,875,694 - +0.5 4,875,713 - +0.8 4,875,743 - +0.9 4,876,518 - +4.6 4,876,539 - +5.9 4,876,539 - +6.3 4,876,639 - +6.1 4,876,639 - +5.9 4,876,720 + SO4678 0.16 +5.6 4,876,790 - SO4678 0.23 +5.7 4,876,798 + SO4678 0.24 +5.6 4,876,948 + SO4678 0.38 +5.8 4,876,959 - SO4678 0.39 +5.6 4,877,023 - SO4678 0.45 +5.7 4,877,038 - SO4678 0.47 +6.7 4,877,060 - SO4678 0.49 +4.2 4,877,137 - SO4678 0.56 +5.5 4,877,165 + SO4678 0.59 +5.3 4,877,173 - SO4678 0.60 +5.9 4,877,173 - SO4678 0.60 +5.3 4,877,179 + SO4678 0.60 +5.9 4,877,187 - SO4678 0.61 +6.4 4,877,303 + SO4678 0.72 +5.4 4,877,366 + SO4678 0.78 +5.9 4,877,396 + SO4678 0.81 +5.3 4,877,404 - SO4678 0.82 +5.7 4,877,416 + SO4678 0.83 +6.0 4,877,424 - SO4678 0.84 +6.1 4,877,442 - SO4678 0.86 +5.9 4,877,457 - SO4678 0.87 +5.3 4,877,460 + SO4678 0.87 +5.3 4,877,467 + SO4678 0.88 +6.1 4,877,467 + SO4678 0.88 +5.7 4,877,544 - +5.4 4,877,585 + -2.7 4,877,585 + +0.6 4,877,587 + -1.9 4,877,595 + -0.2 4,877,659 + +2.6 4,877,667 - +2.5 4,877,677 - +2.3 4,877,677 - +1.9 4,877,702 - +2.8 4,877,732 + +1.3 4,877,795 - SO4679 0.14 +2.0 4,878,131 - SO4679 0.45 +4.0 4,878,216 - SO4679 0.53 +2.4 4,878,299 + SO4679 0.60 -0.4 4,878,307 - SO4679 0.61 +1.3 4,878,331 + SO4679 0.63 +0.3 4,878,334 - SO4679 0.64 +2.2 4,878,334 - SO4679 0.64 -0.7 4,878,349 - SO4679 0.65 +3.3 4,878,520 + SO4679 0.81 +0.3 4,878,520 + SO4679 0.81 -2.9 4,878,525 + SO4679 0.81 +0.6 4,878,526 - SO4679 0.81 +3.4
Or see this region's nucleotide sequence